The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis
Objective To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of Enterococcus faecalis (E. faecalis).Methods The Man-PTS EIID (mptD) deletion (ΔmptD) and overexpressing (+mptD) strains of E. faecalis were constructed. The contribution of the Man-PTS...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2025.2487944 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849701475009167360 |
|---|---|
| author | Runze Liu Yi Luo Pei Liu Shuo Xiao Wei Fan Bing Fan |
| author_facet | Runze Liu Yi Luo Pei Liu Shuo Xiao Wei Fan Bing Fan |
| author_sort | Runze Liu |
| collection | DOAJ |
| description | Objective To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of Enterococcus faecalis (E. faecalis).Methods The Man-PTS EIID (mptD) deletion (ΔmptD) and overexpressing (+mptD) strains of E. faecalis were constructed. The contribution of the Man-PTS EII to alkaline resistance was investigated by assessing growth and biofilm formation of E. faecalis at pH 7 and 10, as well as changes in intracellular ATP levels, pH, potassium ion concentration, membrane potential and membrane permeability. The effects of mptD on the expression of other Man-PTS EII components and membrane transport-related genes were evaluated by RT-qPCR at pH 10. In addition, six clinical strains were isolated. Three strains of E. faecalis with high to low alkaline resistance were screened, and RNA sequencing was performed to further explore the role of Man-PTS EII in the alkaline resistance.Results mptD enhanced the alkaline resistance of E. faecalis by maintaining pH homeostasis in an alkaline environment. Overexpression of mptD induced membrane hyperpolarization, reduced membrane permeability, decreased intracellular K+ levels, lowered cytoplasmic pH, and elevated ATP production, while the loss of mptD reversed these effects (p < 0.05). mptD coordinated the expression of Man-PTS EII components and upregulated genes encoding membrane transporter components (p < 0.01). All Man-PTS EII components were positively related to the degree of alkaline resistance of E. faecalis (p < 0.05).Conclusion Man-PTS EII is a key factor in the interaction between metabolism and ion membrane transport during the alkaline resistance process of E. faecalis. This study may provide new insights for understanding the alkaline resistance of E. faecalis. |
| format | Article |
| id | doaj-art-dac4d4db63cd4c4bb7fc56781d59d5c9 |
| institution | DOAJ |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-dac4d4db63cd4c4bb7fc56781d59d5c92025-08-20T03:17:55ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2487944The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalisRunze Liu0Yi Luo1Pei Liu2Shuo Xiao3Wei Fan4Bing FanThe State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaObjective To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of Enterococcus faecalis (E. faecalis).Methods The Man-PTS EIID (mptD) deletion (ΔmptD) and overexpressing (+mptD) strains of E. faecalis were constructed. The contribution of the Man-PTS EII to alkaline resistance was investigated by assessing growth and biofilm formation of E. faecalis at pH 7 and 10, as well as changes in intracellular ATP levels, pH, potassium ion concentration, membrane potential and membrane permeability. The effects of mptD on the expression of other Man-PTS EII components and membrane transport-related genes were evaluated by RT-qPCR at pH 10. In addition, six clinical strains were isolated. Three strains of E. faecalis with high to low alkaline resistance were screened, and RNA sequencing was performed to further explore the role of Man-PTS EII in the alkaline resistance.Results mptD enhanced the alkaline resistance of E. faecalis by maintaining pH homeostasis in an alkaline environment. Overexpression of mptD induced membrane hyperpolarization, reduced membrane permeability, decreased intracellular K+ levels, lowered cytoplasmic pH, and elevated ATP production, while the loss of mptD reversed these effects (p < 0.05). mptD coordinated the expression of Man-PTS EII components and upregulated genes encoding membrane transporter components (p < 0.01). All Man-PTS EII components were positively related to the degree of alkaline resistance of E. faecalis (p < 0.05).Conclusion Man-PTS EII is a key factor in the interaction between metabolism and ion membrane transport during the alkaline resistance process of E. faecalis. This study may provide new insights for understanding the alkaline resistance of E. faecalis.https://www.tandfonline.com/doi/10.1080/20002297.2025.2487944Alkaline resistancephosphotransferase systemmannoseEnterococcus faecalis |
| spellingShingle | Runze Liu Yi Luo Pei Liu Shuo Xiao Wei Fan Bing Fan The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis Journal of Oral Microbiology Alkaline resistance phosphotransferase system mannose Enterococcus faecalis |
| title | The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis |
| title_full | The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis |
| title_fullStr | The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis |
| title_full_unstemmed | The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis |
| title_short | The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of Enterococcus faecalis |
| title_sort | potential regulatory role of mannose phosphotransferase system eii in alkaline resistance of enterococcus faecalis |
| topic | Alkaline resistance phosphotransferase system mannose Enterococcus faecalis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2025.2487944 |
| work_keys_str_mv | AT runzeliu thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT yiluo thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT peiliu thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT shuoxiao thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT weifan thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT bingfan thepotentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT runzeliu potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT yiluo potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT peiliu potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT shuoxiao potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT weifan potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis AT bingfan potentialregulatoryroleofmannosephosphotransferasesystemeiiinalkalineresistanceofenterococcusfaecalis |