Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration
This study explores the impact of initial substrate concentrations on biomass growth, hydrogen production, and acid generation during acidogenic fermentation of a synthetic fruit juice wastewater. Four substrate concentrations, within the range 0.30–2.12 C mol·L<sup>−1</sup>, were tested...
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MDPI AG
2024-11-01
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| author | Encarnación Díaz Domínguez María Eugenia Ibañez López José Luis García Morales Ester López-Fernández Francisco Jesús Fernández-Morales |
| author_facet | Encarnación Díaz Domínguez María Eugenia Ibañez López José Luis García Morales Ester López-Fernández Francisco Jesús Fernández-Morales |
| author_sort | Encarnación Díaz Domínguez |
| collection | DOAJ |
| description | This study explores the impact of initial substrate concentrations on biomass growth, hydrogen production, and acid generation during acidogenic fermentation of a synthetic fruit juice wastewater. Four substrate concentrations, within the range 0.30–2.12 C mol·L<sup>−1</sup>, were tested using a mixed acidogenic bacterial culture. Batch reactors were employed to conduct the study, and the results were analyzed to determine inhibition effects. The highest hydrogen production (6.1 L H<sub>2</sub>·substrate C mol<sup>−1</sup>) and hydrogen percentage in the gas phase (57%) were achieved at a substrate concentration of 0.30 C mol·L<sup>−1</sup>. Higher substrate concentrations reduced the hydrogen production due to substrate and product inhibition events. The maximum H<sub>2</sub> potential production was 4.15 Nm<sup>3</sup>/m<sup>3</sup> reactor at a substrate concentration of 0.91 C mol·L<sup>−1</sup>. Biomass growth and VFA production followed exponential trends at low substrate concentrations, 0.30 and 0.091 C mol·L<sup>−1</sup>, while high concentrations resulted in linear trends due to inhibition effects caused by the substrate. The main acids produced were lactic at low concentrations, and acetic when dealing with high concentrations. The highest final acid concentrations were obtained with the highest initial substrate concentration, but their yields were significantly lower due to the substrate and product inhibitions experienced by the biomass. |
| format | Article |
| id | doaj-art-dac424c50fda455fb651592b1c0880ee |
| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-dac424c50fda455fb651592b1c0880ee2025-08-20T01:53:49ZengMDPI AGApplied Sciences2076-34172024-11-0114221051910.3390/app142210519Dark Fermentation of Fruit Juice Effluents: Effects of Substrate ConcentrationEncarnación Díaz Domínguez0María Eugenia Ibañez López1José Luis García Morales2Ester López-Fernández3Francisco Jesús Fernández-Morales4Department of Environmental Technologies, Faculty of Marine and Environmental Sciences, IVAGRO-Wine and Agrifood Research Institute, University of Cadiz, 11510 Cadiz, SpainDepartment of Environmental Technologies, Faculty of Marine and Environmental Sciences, IVAGRO-Wine and Agrifood Research Institute, University of Cadiz, 11510 Cadiz, SpainDepartment of Environmental Technologies, Faculty of Marine and Environmental Sciences, IVAGRO-Wine and Agrifood Research Institute, University of Cadiz, 11510 Cadiz, SpainDepartment of Chemical Engineering, University of Castilla-La Mancha, Avda. Camilo José Cela S/N, 13071 Ciudad Real, SpainDepartment of Chemical Engineering, University of Castilla-La Mancha, Avda. Camilo José Cela S/N, 13071 Ciudad Real, SpainThis study explores the impact of initial substrate concentrations on biomass growth, hydrogen production, and acid generation during acidogenic fermentation of a synthetic fruit juice wastewater. Four substrate concentrations, within the range 0.30–2.12 C mol·L<sup>−1</sup>, were tested using a mixed acidogenic bacterial culture. Batch reactors were employed to conduct the study, and the results were analyzed to determine inhibition effects. The highest hydrogen production (6.1 L H<sub>2</sub>·substrate C mol<sup>−1</sup>) and hydrogen percentage in the gas phase (57%) were achieved at a substrate concentration of 0.30 C mol·L<sup>−1</sup>. Higher substrate concentrations reduced the hydrogen production due to substrate and product inhibition events. The maximum H<sub>2</sub> potential production was 4.15 Nm<sup>3</sup>/m<sup>3</sup> reactor at a substrate concentration of 0.91 C mol·L<sup>−1</sup>. Biomass growth and VFA production followed exponential trends at low substrate concentrations, 0.30 and 0.091 C mol·L<sup>−1</sup>, while high concentrations resulted in linear trends due to inhibition effects caused by the substrate. The main acids produced were lactic at low concentrations, and acetic when dealing with high concentrations. The highest final acid concentrations were obtained with the highest initial substrate concentration, but their yields were significantly lower due to the substrate and product inhibitions experienced by the biomass.https://www.mdpi.com/2076-3417/14/22/10519acidogenic fermentationhydrogen productionvolatile fatty acidsinhibition |
| spellingShingle | Encarnación Díaz Domínguez María Eugenia Ibañez López José Luis García Morales Ester López-Fernández Francisco Jesús Fernández-Morales Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration Applied Sciences acidogenic fermentation hydrogen production volatile fatty acids inhibition |
| title | Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration |
| title_full | Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration |
| title_fullStr | Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration |
| title_full_unstemmed | Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration |
| title_short | Dark Fermentation of Fruit Juice Effluents: Effects of Substrate Concentration |
| title_sort | dark fermentation of fruit juice effluents effects of substrate concentration |
| topic | acidogenic fermentation hydrogen production volatile fatty acids inhibition |
| url | https://www.mdpi.com/2076-3417/14/22/10519 |
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