Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia
Abstract About 5% of the population suffers from fibromyalgia (FM), a chronic multi-symptom pain illness whose pathophysiology is still unknown. We aimed to be the first to investigate the possible association of sera levels of miR-217 and miR-532 in patients with fibromyalgia and correlate their ex...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02330-y |
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| author | Shymaa E. Ayoub Azza M. Ahmed Mostafa Y. Abdelwahed Abeer A. Khalefa Aeshah A. Awaji Samer S. Zekry Enas G. Ibrahim Hana M. Eid Shimaa M. Elasmer Reham Fares |
| author_facet | Shymaa E. Ayoub Azza M. Ahmed Mostafa Y. Abdelwahed Abeer A. Khalefa Aeshah A. Awaji Samer S. Zekry Enas G. Ibrahim Hana M. Eid Shimaa M. Elasmer Reham Fares |
| author_sort | Shymaa E. Ayoub |
| collection | DOAJ |
| description | Abstract About 5% of the population suffers from fibromyalgia (FM), a chronic multi-symptom pain illness whose pathophysiology is still unknown. We aimed to be the first to investigate the possible association of sera levels of miR-217 and miR-532 in patients with fibromyalgia and correlate their expression levels to different clinical and biochemical disease criteria. This study included 80 participants who splitted into two groups: 40 fibromyalgia sufferers (12 male and 28 female), and 40 healthy volunteers (10 male and 30 female) who served as the control group. Venous blood samples were collected from all subjects. The miR-217 and miR-532 serum expressions were detected using quantitative real-time PCR (qRT-PCR). According to our data, the fold changes of miR-217 and miR-532 in fibromyalgia patients were significantly lower than in controls, for miR-217 (median = 0.1359, IQR: 0.038–0.287, P < 0.001) and miR-532 [median = 0.2199, IQR: (0.114–0.421), P < 0.001]. In addition, there was a significant negative relationship between Aspartate transaminase (AST) and both miR-217 and miR-532 (r = − 0.480, P = P < 0.001 r = − 0.462, P = P < 0.001), respectively. Serum miR-217 and miR-532 could serve as potential diagnostic biomarkers for fibromyalgia. |
| format | Article |
| id | doaj-art-dabe914df5f141faa60c734dfabb496e |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-dabe914df5f141faa60c734dfabb496e2025-02-09T12:26:32ZengBMCEuropean Journal of Medical Research2047-783X2025-02-013011610.1186/s40001-025-02330-yBiochemical analysis of miR-217 and miR-532 in patients with fibromyalgiaShymaa E. Ayoub0Azza M. Ahmed1Mostafa Y. Abdelwahed2Abeer A. Khalefa3Aeshah A. Awaji4Samer S. Zekry5Enas G. Ibrahim6Hana M. Eid7Shimaa M. Elasmer8Reham Fares9Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Fayoum UniversityRheumatology Department, Faculty of Medicine, Fayoum UniversityDepartment of Physiology, Faculty of Medicine, Fayoum UniversityDepartment of Physiology, Faculty of Medicine, Zagazig UniversityDepartment of Biology, Faculty of Science, University College of Taymaa, University of TabukDepartment of Internal Medicine, Faculty of Medicine, Fayoum UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum UniversityDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Fayoum UniversityMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Fayoum UniversityAbstract About 5% of the population suffers from fibromyalgia (FM), a chronic multi-symptom pain illness whose pathophysiology is still unknown. We aimed to be the first to investigate the possible association of sera levels of miR-217 and miR-532 in patients with fibromyalgia and correlate their expression levels to different clinical and biochemical disease criteria. This study included 80 participants who splitted into two groups: 40 fibromyalgia sufferers (12 male and 28 female), and 40 healthy volunteers (10 male and 30 female) who served as the control group. Venous blood samples were collected from all subjects. The miR-217 and miR-532 serum expressions were detected using quantitative real-time PCR (qRT-PCR). According to our data, the fold changes of miR-217 and miR-532 in fibromyalgia patients were significantly lower than in controls, for miR-217 (median = 0.1359, IQR: 0.038–0.287, P < 0.001) and miR-532 [median = 0.2199, IQR: (0.114–0.421), P < 0.001]. In addition, there was a significant negative relationship between Aspartate transaminase (AST) and both miR-217 and miR-532 (r = − 0.480, P = P < 0.001 r = − 0.462, P = P < 0.001), respectively. Serum miR-217 and miR-532 could serve as potential diagnostic biomarkers for fibromyalgia.https://doi.org/10.1186/s40001-025-02330-yFibromyalgiaMiR-217MiR-532 |
| spellingShingle | Shymaa E. Ayoub Azza M. Ahmed Mostafa Y. Abdelwahed Abeer A. Khalefa Aeshah A. Awaji Samer S. Zekry Enas G. Ibrahim Hana M. Eid Shimaa M. Elasmer Reham Fares Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia European Journal of Medical Research Fibromyalgia MiR-217 MiR-532 |
| title | Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia |
| title_full | Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia |
| title_fullStr | Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia |
| title_full_unstemmed | Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia |
| title_short | Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia |
| title_sort | biochemical analysis of mir 217 and mir 532 in patients with fibromyalgia |
| topic | Fibromyalgia MiR-217 MiR-532 |
| url | https://doi.org/10.1186/s40001-025-02330-y |
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