Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®”
Background: “Nepic®” (Japan Tissue Engineering Co., Ltd., Gamagori, Japan) is an autologous cultivated limbal epithelial sheet, approved in 2020 in Japan for the reconstruction of the corneal surface in cases of limbal stem cell deficiency (LSCD). Because the surgical procedure known as cultivated l...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fujita Medical Society
2025-08-01
|
| Series: | Fujita Medical Journal |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/fmj/11/3/11_2024-031/_pdf/-char/en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850070548680278016 |
|---|---|
| author | Daisuke Kato Koji Hirano Atsuhiro Tanikawa Yasuki Ito |
| author_facet | Daisuke Kato Koji Hirano Atsuhiro Tanikawa Yasuki Ito |
| author_sort | Daisuke Kato |
| collection | DOAJ |
| description | Background: “Nepic®” (Japan Tissue Engineering Co., Ltd., Gamagori, Japan) is an autologous cultivated limbal epithelial sheet, approved in 2020 in Japan for the reconstruction of the corneal surface in cases of limbal stem cell deficiency (LSCD). Because the surgical procedure known as cultivated limbal epithelial cell sheet transplantation (CLET) has only recently been introduced and the number of cases remains limited, accumulating clinical experience is essential to ensure the safety and success of this procedure. Herein, we report the clinical course of a patient with unilateral LSCD who underwent CLET for a corneal surface reconstruction using Nepic®.
Case presentation: A 50-year-old man sustained bilateral eye injuries from mortar during construction work. The patient’s right cornea exhibited LSCD. Initial treatment involved a 360° limbal transplantation with an allo-corneal graft at a university hospital. However, graft rejection occurred, and the corneal surface was subsequently covered by conjunctival tissue within 2 months. Because the patient’s left cornea and conjunctiva appeared healthy, we performed CLET with Nepic® 3 years after the limbal transplantation.
Conclusions: When using Nepic®, it is essential to become accustomed to handling the cultivated epithelial sheet on the ring-shaped holder; however, cultivated corneal epithelium transplantation can also be performed without the carrier material, but rather as a sheet. Though the corneal surface appeared smooth and shiny at 7 months postoperatively, we seek to determine whether the epithelial cells on the patient’s cornea are of corneal or conjunctival origin without resorting to invasive procedures, such as biopsy. |
| format | Article |
| id | doaj-art-dab6442faae3452d91f59845649ca711 |
| institution | DOAJ |
| issn | 2189-7247 2189-7255 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Fujita Medical Society |
| record_format | Article |
| series | Fujita Medical Journal |
| spelling | doaj-art-dab6442faae3452d91f59845649ca7112025-08-20T02:47:31ZengFujita Medical SocietyFujita Medical Journal2189-72472189-72552025-08-01113142145https://doi.org/10.20407/fmj.2024-031Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®”Daisuke Kato0Koji Hirano1Atsuhiro Tanikawa2Yasuki Ito3Department of Ophthalmology, Fujita Health University, School of MedicineDepartment of Ophthalmology, Fujita Health University, School of Medicine; Department of Ophthalmology, TOYOTA Memorial HospitalDepartment of Ophthalmology, Fujita Health University Bantane HospitalDepartment of Ophthalmology, Fujita Health University, School of MedicineBackground: “Nepic®” (Japan Tissue Engineering Co., Ltd., Gamagori, Japan) is an autologous cultivated limbal epithelial sheet, approved in 2020 in Japan for the reconstruction of the corneal surface in cases of limbal stem cell deficiency (LSCD). Because the surgical procedure known as cultivated limbal epithelial cell sheet transplantation (CLET) has only recently been introduced and the number of cases remains limited, accumulating clinical experience is essential to ensure the safety and success of this procedure. Herein, we report the clinical course of a patient with unilateral LSCD who underwent CLET for a corneal surface reconstruction using Nepic®. Case presentation: A 50-year-old man sustained bilateral eye injuries from mortar during construction work. The patient’s right cornea exhibited LSCD. Initial treatment involved a 360° limbal transplantation with an allo-corneal graft at a university hospital. However, graft rejection occurred, and the corneal surface was subsequently covered by conjunctival tissue within 2 months. Because the patient’s left cornea and conjunctiva appeared healthy, we performed CLET with Nepic® 3 years after the limbal transplantation. Conclusions: When using Nepic®, it is essential to become accustomed to handling the cultivated epithelial sheet on the ring-shaped holder; however, cultivated corneal epithelium transplantation can also be performed without the carrier material, but rather as a sheet. Though the corneal surface appeared smooth and shiny at 7 months postoperatively, we seek to determine whether the epithelial cells on the patient’s cornea are of corneal or conjunctival origin without resorting to invasive procedures, such as biopsy.https://www.jstage.jst.go.jp/article/fmj/11/3/11_2024-031/_pdf/-char/enchemical injurycultivated limbal epithelial cell sheet transplantation (clet)nepic®unilateral limbal stem cell deficiency |
| spellingShingle | Daisuke Kato Koji Hirano Atsuhiro Tanikawa Yasuki Ito Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” Fujita Medical Journal chemical injury cultivated limbal epithelial cell sheet transplantation (clet) nepic® unilateral limbal stem cell deficiency |
| title | Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” |
| title_full | Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” |
| title_fullStr | Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” |
| title_full_unstemmed | Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” |
| title_short | Corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet “Nepic®” |
| title_sort | corneal surface reconstruction for the chemical injured eye by transplanting autologous cultivated limbal epithelial sheet nepic r |
| topic | chemical injury cultivated limbal epithelial cell sheet transplantation (clet) nepic® unilateral limbal stem cell deficiency |
| url | https://www.jstage.jst.go.jp/article/fmj/11/3/11_2024-031/_pdf/-char/en |
| work_keys_str_mv | AT daisukekato cornealsurfacereconstructionforthechemicalinjuredeyebytransplantingautologouscultivatedlimbalepithelialsheetnepic AT kojihirano cornealsurfacereconstructionforthechemicalinjuredeyebytransplantingautologouscultivatedlimbalepithelialsheetnepic AT atsuhirotanikawa cornealsurfacereconstructionforthechemicalinjuredeyebytransplantingautologouscultivatedlimbalepithelialsheetnepic AT yasukiito cornealsurfacereconstructionforthechemicalinjuredeyebytransplantingautologouscultivatedlimbalepithelialsheetnepic |