In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts
Abstract Background Bacterial and helminthic evolution has resulted in a shocking development of antibiotic and anthelminthic resistance. Medicinal plants provide an encouraging source to develop new antibacterial and anthelmintic agents. Rubus ellipticus has been employed traditionally for medicina...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-06-01
|
| Series: | Discover Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s44371-025-00227-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849432982213885952 |
|---|---|
| author | Deepak Kumar Adarsh Kumar Ankit Kumar Singh Harshwardhan Singh Pradeep Kumar |
| author_facet | Deepak Kumar Adarsh Kumar Ankit Kumar Singh Harshwardhan Singh Pradeep Kumar |
| author_sort | Deepak Kumar |
| collection | DOAJ |
| description | Abstract Background Bacterial and helminthic evolution has resulted in a shocking development of antibiotic and anthelminthic resistance. Medicinal plants provide an encouraging source to develop new antibacterial and anthelmintic agents. Rubus ellipticus has been employed traditionally for medicinal purposes, yet its ability against microbial resistance remains to be unraveled. Methods The present work assessed the antioxidant, antibacterial, and anthelmintic activities of leaf extracts of R. ellipticus. Antioxidant activity was measured using DPPH, antibacterial activity was done with the serial dilution technique, and anthelmintic activity was performed by using Indian earthworms. Molecular docking experiments were also carried out by utilizing Maestro 12.7 (Schrodinger) in order to screen the interaction between phytoconstituents with bacterial as well as helminthic targets. Results The methanol extract exhibited the highest antioxidant activity (%RSA = 90.95 ± 0.47%) and was the most potent antibacterial, with a minimum inhibitory concentration (MIC) value of 12.5 µg/mL against Bacillus subtilis and Escherichia coli. Paralysis and death time of earthworms were significantly reduced by methanol and acetone extracts at 20 mg/mL (paralysis: 12 ± 1.00 min, death: 15.33 ± 1.20 min). The molecular docking outcomes showed that kaempferol, ellagic acid, gallic acid, rutin, anthocyanin, and quercetin had good binding affinities towards the target proteins. Conclusion The results indicated that R. ellipticus is a potential natural source of bioactive compounds with antibacterial and anthelmintic activity. Additional research, including in vivo and mechanistic studies, is suggested to confirm its therapeutic uses. |
| format | Article |
| id | doaj-art-dab4fe8efb6b47a5a7482efa57b76734 |
| institution | Kabale University |
| issn | 3005-1193 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Chemistry |
| spelling | doaj-art-dab4fe8efb6b47a5a7482efa57b767342025-08-20T03:27:13ZengSpringerDiscover Chemistry3005-11932025-06-012111410.1007/s44371-025-00227-7In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extractsDeepak Kumar0Adarsh Kumar1Ankit Kumar Singh2Harshwardhan Singh3Pradeep Kumar4Department of Pharmaceutical Sciences and Natural Products, Central University of PunjabDepartment of Pharmaceutical Sciences and Natural Products, Central University of PunjabDepartment of Pharmaceutical Sciences and Natural Products, Central University of PunjabDepartment of Pharmaceutical Sciences and Natural Products, Central University of PunjabDepartment of Pharmaceutical Sciences and Natural Products, Central University of PunjabAbstract Background Bacterial and helminthic evolution has resulted in a shocking development of antibiotic and anthelminthic resistance. Medicinal plants provide an encouraging source to develop new antibacterial and anthelmintic agents. Rubus ellipticus has been employed traditionally for medicinal purposes, yet its ability against microbial resistance remains to be unraveled. Methods The present work assessed the antioxidant, antibacterial, and anthelmintic activities of leaf extracts of R. ellipticus. Antioxidant activity was measured using DPPH, antibacterial activity was done with the serial dilution technique, and anthelmintic activity was performed by using Indian earthworms. Molecular docking experiments were also carried out by utilizing Maestro 12.7 (Schrodinger) in order to screen the interaction between phytoconstituents with bacterial as well as helminthic targets. Results The methanol extract exhibited the highest antioxidant activity (%RSA = 90.95 ± 0.47%) and was the most potent antibacterial, with a minimum inhibitory concentration (MIC) value of 12.5 µg/mL against Bacillus subtilis and Escherichia coli. Paralysis and death time of earthworms were significantly reduced by methanol and acetone extracts at 20 mg/mL (paralysis: 12 ± 1.00 min, death: 15.33 ± 1.20 min). The molecular docking outcomes showed that kaempferol, ellagic acid, gallic acid, rutin, anthocyanin, and quercetin had good binding affinities towards the target proteins. Conclusion The results indicated that R. ellipticus is a potential natural source of bioactive compounds with antibacterial and anthelmintic activity. Additional research, including in vivo and mechanistic studies, is suggested to confirm its therapeutic uses.https://doi.org/10.1007/s44371-025-00227-7Rubus ellipticusAntibacterialAnthelminticMolecular dockingAntioxidant |
| spellingShingle | Deepak Kumar Adarsh Kumar Ankit Kumar Singh Harshwardhan Singh Pradeep Kumar In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts Discover Chemistry Rubus ellipticus Antibacterial Anthelmintic Molecular docking Antioxidant |
| title | In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts |
| title_full | In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts |
| title_fullStr | In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts |
| title_full_unstemmed | In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts |
| title_short | In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts |
| title_sort | in vitro and in silico anthelmintic and antibacterial activity of rubus ellipticus leaf extracts |
| topic | Rubus ellipticus Antibacterial Anthelmintic Molecular docking Antioxidant |
| url | https://doi.org/10.1007/s44371-025-00227-7 |
| work_keys_str_mv | AT deepakkumar invitroandinsilicoanthelminticandantibacterialactivityofrubusellipticusleafextracts AT adarshkumar invitroandinsilicoanthelminticandantibacterialactivityofrubusellipticusleafextracts AT ankitkumarsingh invitroandinsilicoanthelminticandantibacterialactivityofrubusellipticusleafextracts AT harshwardhansingh invitroandinsilicoanthelminticandantibacterialactivityofrubusellipticusleafextracts AT pradeepkumar invitroandinsilicoanthelminticandantibacterialactivityofrubusellipticusleafextracts |