DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq
Introduction: There are different ways for Tobacco consumption among smokers. The main three ways for smoking are Cigarettes, Cigar and Narghile (the waterpipie). Narghile smoking has become a very popular habit among youth in different countries around the world. Results: There is higher frequency...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Oral Oncology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906022000024 |
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| author | Hishyar Azo Najeeb Karzan Khowaraham Karim Galawezh Obaid Othman Ayad Ahmad Mohammed Ahmed Mohammed Salih Soleen Sardar Zuhdi Dian Jamel Salih |
| author_facet | Hishyar Azo Najeeb Karzan Khowaraham Karim Galawezh Obaid Othman Ayad Ahmad Mohammed Ahmed Mohammed Salih Soleen Sardar Zuhdi Dian Jamel Salih |
| author_sort | Hishyar Azo Najeeb |
| collection | DOAJ |
| description | Introduction: There are different ways for Tobacco consumption among smokers. The main three ways for smoking are Cigarettes, Cigar and Narghile (the waterpipie). Narghile smoking has become a very popular habit among youth in different countries around the world. Results: There is higher frequency of micronuclei formation in cigarettes and Narghile smokers compared to control group (7.1 ± 0.9, 11.2 ± 0.4, and 2.2 ± 0.7 respectively). The mean frequency of the DNA fading (Karyolysis) in Narghile smokers was 29-fold higher than non-smokers. The mean frequencies of Karyorhexis buccal cells in Narghile smokers were 1.9-fold higher than non-smokers. The mean of Pyknosis formation was 19-fold greater than non-smokers. More migrated DNA tails were found Narghile smokers compared to controls. The mean of DNA damage measured by ACA was significantly greater (p < 0.0001) than in non-smokers (53.32 ± 3.435 N = 26 vs. 22.61 ± 1.909 N = 21, respectively). Narghile smoking increased frequencies of micronucleus formation in buccal cells of Narghile smokers (p < 0.005) compared to cigarettes smokers and non-smokers. The mean of DNA damage measured in peripheral blood cells of Narghile smokers was significantly greater (p < 0.0001) than in non-smokers. Conclusion: In our study we have found direct and accumulative genomic damage effect of smoking in cigarette and Narghile smokers. Narghile smoking causes more DNA damage in buccal cells and in peripheral blood cells. This could be an evidence indicating that there is accumulative effect of Narghile smoking on genomic stability in peripheral blood cells and as well as in buccal epithelial cells. |
| format | Article |
| id | doaj-art-daa9ded19f85491380d93e2c1317e6e8 |
| institution | OA Journals |
| issn | 2772-9060 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-daa9ded19f85491380d93e2c1317e6e82025-08-20T02:02:55ZengElsevierOral Oncology Reports2772-90602022-03-01110000210.1016/j.oor.2022.100002DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-IraqHishyar Azo Najeeb0Karzan Khowaraham Karim1Galawezh Obaid Othman2Ayad Ahmad Mohammed3Ahmed Mohammed Salih4Soleen Sardar Zuhdi5Dian Jamel Salih6Department of Medical Chemistry, College of Medicine, University of Duhok, Kurdistan Region, IraqEducation and Higher Education Ministries, Kurdistan Region, IraqDepartment of Biology, College of Education, Salahaddin University, Erbil, Kurdistan Region, IraqDepartment of Surgery, College of Medicine, University of Duhok, Kurdistan Region, Iraq; Corresponding author. Department of Surgery, College of Medicine, University of Duhok, Nakhoshkhana Road, 8 AM-1014, Duhok, Kurdistan Region, Iraq.Duhok Medical Research Center, College of Medicine, University of Duhok, Kurdistan Region, IraqDuhok Medical Research Center, College of Medicine, University of Duhok, Kurdistan Region, IraqDepartment of Biology, College of Medicine, University of Duhok, Kurdistan Region, IraqIntroduction: There are different ways for Tobacco consumption among smokers. The main three ways for smoking are Cigarettes, Cigar and Narghile (the waterpipie). Narghile smoking has become a very popular habit among youth in different countries around the world. Results: There is higher frequency of micronuclei formation in cigarettes and Narghile smokers compared to control group (7.1 ± 0.9, 11.2 ± 0.4, and 2.2 ± 0.7 respectively). The mean frequency of the DNA fading (Karyolysis) in Narghile smokers was 29-fold higher than non-smokers. The mean frequencies of Karyorhexis buccal cells in Narghile smokers were 1.9-fold higher than non-smokers. The mean of Pyknosis formation was 19-fold greater than non-smokers. More migrated DNA tails were found Narghile smokers compared to controls. The mean of DNA damage measured by ACA was significantly greater (p < 0.0001) than in non-smokers (53.32 ± 3.435 N = 26 vs. 22.61 ± 1.909 N = 21, respectively). Narghile smoking increased frequencies of micronucleus formation in buccal cells of Narghile smokers (p < 0.005) compared to cigarettes smokers and non-smokers. The mean of DNA damage measured in peripheral blood cells of Narghile smokers was significantly greater (p < 0.0001) than in non-smokers. Conclusion: In our study we have found direct and accumulative genomic damage effect of smoking in cigarette and Narghile smokers. Narghile smoking causes more DNA damage in buccal cells and in peripheral blood cells. This could be an evidence indicating that there is accumulative effect of Narghile smoking on genomic stability in peripheral blood cells and as well as in buccal epithelial cells.http://www.sciencedirect.com/science/article/pii/S2772906022000024GeneticsCytotoxicityDNA repair mechanism mutationsNargileSmokingDNA damage |
| spellingShingle | Hishyar Azo Najeeb Karzan Khowaraham Karim Galawezh Obaid Othman Ayad Ahmad Mohammed Ahmed Mohammed Salih Soleen Sardar Zuhdi Dian Jamel Salih DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq Oral Oncology Reports Genetics Cytotoxicity DNA repair mechanism mutations Nargile Smoking DNA damage |
| title | DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq |
| title_full | DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq |
| title_fullStr | DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq |
| title_full_unstemmed | DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq |
| title_short | DNA damage level in lymphocytes and buccal epithelial cells in Narghile smokers and non-smokers in Duhok city-KRG-Iraq |
| title_sort | dna damage level in lymphocytes and buccal epithelial cells in narghile smokers and non smokers in duhok city krg iraq |
| topic | Genetics Cytotoxicity DNA repair mechanism mutations Nargile Smoking DNA damage |
| url | http://www.sciencedirect.com/science/article/pii/S2772906022000024 |
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