Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women
Higher in vivo fatty acid (FA) oxidation rates have been reported in obese individuals compared to lean counterparts; however whether this reflects a shift in substrate-specific oxidative capacity at the level of the skeletal muscle mitochondria has not been examined. The purpose of this study was t...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2017/7832057 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849472716144377856 |
|---|---|
| author | Jonathan L. Warren Barbara A. Gower Gary R. Hunter Samuel T. Windham Douglas R. Moellering Gordon Fisher |
| author_facet | Jonathan L. Warren Barbara A. Gower Gary R. Hunter Samuel T. Windham Douglas R. Moellering Gordon Fisher |
| author_sort | Jonathan L. Warren |
| collection | DOAJ |
| description | Higher in vivo fatty acid (FA) oxidation rates have been reported in obese individuals compared to lean counterparts; however whether this reflects a shift in substrate-specific oxidative capacity at the level of the skeletal muscle mitochondria has not been examined. The purpose of this study was to test the hypothesis that in situ measures of skeletal muscle mitochondria FA oxidation would be positively associated with total body fat. Participants were 38 premenopausal women (BMI=26.5±4.3 kg/m2). Total and regional fat were assessed by dual-energy X-ray absorptiometry (DXA). Mitochondrial FA oxidation was assessed in permeabilized myofibers using high-resolution respirometry and a palmitoyl carnitine substrate. We found positive associations of total fat mass with State 3 (ADP-stimulated respiration) (r=0.379, p<0.05) and the respiratory control ratio (RCR, measure of mitochondrial coupling) (r=0.348, p<0.05). When participants were dichotomized by high or low body fat percent, participants with high total body fat displayed a higher RCR compared to those with low body fat (p<0.05). There were no associations between any measure of regional fat and mitochondrial FA oxidation independent of total fat mass. In conclusion, greater FA oxidation in obesity may reflect molecular processes that enhance FA oxidation capacity at the mitochondrial level. |
| format | Article |
| id | doaj-art-daa977dcee7b41cf8a443fffcd42ff10 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-daa977dcee7b41cf8a443fffcd42ff102025-08-20T03:24:26ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322017-01-01201710.1155/2017/78320577832057Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal WomenJonathan L. Warren0Barbara A. Gower1Gary R. Hunter2Samuel T. Windham3Douglas R. Moellering4Gordon Fisher5Department of Nutrition Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USADepartment of Nutrition Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USADepartment of Human Studies, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USADepartment of Surgery, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USADepartment of Nutrition Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USADepartment of Human Studies, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USAHigher in vivo fatty acid (FA) oxidation rates have been reported in obese individuals compared to lean counterparts; however whether this reflects a shift in substrate-specific oxidative capacity at the level of the skeletal muscle mitochondria has not been examined. The purpose of this study was to test the hypothesis that in situ measures of skeletal muscle mitochondria FA oxidation would be positively associated with total body fat. Participants were 38 premenopausal women (BMI=26.5±4.3 kg/m2). Total and regional fat were assessed by dual-energy X-ray absorptiometry (DXA). Mitochondrial FA oxidation was assessed in permeabilized myofibers using high-resolution respirometry and a palmitoyl carnitine substrate. We found positive associations of total fat mass with State 3 (ADP-stimulated respiration) (r=0.379, p<0.05) and the respiratory control ratio (RCR, measure of mitochondrial coupling) (r=0.348, p<0.05). When participants were dichotomized by high or low body fat percent, participants with high total body fat displayed a higher RCR compared to those with low body fat (p<0.05). There were no associations between any measure of regional fat and mitochondrial FA oxidation independent of total fat mass. In conclusion, greater FA oxidation in obesity may reflect molecular processes that enhance FA oxidation capacity at the mitochondrial level.http://dx.doi.org/10.1155/2017/7832057 |
| spellingShingle | Jonathan L. Warren Barbara A. Gower Gary R. Hunter Samuel T. Windham Douglas R. Moellering Gordon Fisher Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women Journal of Nutrition and Metabolism |
| title | Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women |
| title_full | Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women |
| title_fullStr | Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women |
| title_full_unstemmed | Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women |
| title_short | Associations of Mitochondrial Fatty Acid Oxidation with Body Fat in Premenopausal Women |
| title_sort | associations of mitochondrial fatty acid oxidation with body fat in premenopausal women |
| url | http://dx.doi.org/10.1155/2017/7832057 |
| work_keys_str_mv | AT jonathanlwarren associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen AT barbaraagower associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen AT garyrhunter associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen AT samueltwindham associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen AT douglasrmoellering associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen AT gordonfisher associationsofmitochondrialfattyacidoxidationwithbodyfatinpremenopausalwomen |