Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule
This study aims to investigate Capsa, an ingestible capsule designed to collect the contents of the small intestine as it passes through the gastrointestinal tract. Eight Swiss Large White pigs weighing between 52.5 and 71.3 kg were administered two capsules each and monitored for three days before...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2024.2427758 |
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| author | Inés García Viñado Federico Correa Paolo Trevisi Giuseppe Bee Catherine Ollagnier |
| author_facet | Inés García Viñado Federico Correa Paolo Trevisi Giuseppe Bee Catherine Ollagnier |
| author_sort | Inés García Viñado |
| collection | DOAJ |
| description | This study aims to investigate Capsa, an ingestible capsule designed to collect the contents of the small intestine as it passes through the gastrointestinal tract. Eight Swiss Large White pigs weighing between 52.5 and 71.3 kg were administered two capsules each and monitored for three days before euthanasia for post-mortem sampling. Samples were collected from six equally divided segments of the small intestine, along with separate sampling of the solid and liquid contents of each segment when feasible. Samples were also obtained from the large intestine and faeces to determine CapSa’s sampling location. Fifteen capsules were retrieved from faecal samples (93.75%), with 87.5% recovered on the first day post-administration. Only one capsule was not recovered. Comparative analysis of the bacterial composition of the capsules and post-mortem samples was conducted using a Permutational Multivariate Analysis of Variance (PERMANOVA) model (Adonis), with sample type as a factor. The results revealed significant differences in bacterial composition between capsules and samples from the large intestine and faeces (p < 0.01). However, no significant difference was observed between capsule content and the liquid and solid parts of the fourth segment of the small intestine (p > 0.05). This study provides evidence that CapSa can effectively sample the intestinal microbiota of the middle part of the small intestine in growing pigs. |
| format | Article |
| id | doaj-art-daa58b7f319145d2bec7aec069cf2d49 |
| institution | DOAJ |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-daa58b7f319145d2bec7aec069cf2d492025-08-20T02:42:53ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2024-12-012311764177310.1080/1828051X.2024.24277582427758Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsuleInés García Viñado0Federico Correa1Paolo Trevisi2Giuseppe Bee3Catherine Ollagnier4Pig Research Unit, AgroscopeDepartment of Agricultural and Food Sciences (DISTAL), University of BolognaDepartment of Agricultural and Food Sciences (DISTAL), University of BolognaPig Research Unit, AgroscopePig Research Unit, AgroscopeThis study aims to investigate Capsa, an ingestible capsule designed to collect the contents of the small intestine as it passes through the gastrointestinal tract. Eight Swiss Large White pigs weighing between 52.5 and 71.3 kg were administered two capsules each and monitored for three days before euthanasia for post-mortem sampling. Samples were collected from six equally divided segments of the small intestine, along with separate sampling of the solid and liquid contents of each segment when feasible. Samples were also obtained from the large intestine and faeces to determine CapSa’s sampling location. Fifteen capsules were retrieved from faecal samples (93.75%), with 87.5% recovered on the first day post-administration. Only one capsule was not recovered. Comparative analysis of the bacterial composition of the capsules and post-mortem samples was conducted using a Permutational Multivariate Analysis of Variance (PERMANOVA) model (Adonis), with sample type as a factor. The results revealed significant differences in bacterial composition between capsules and samples from the large intestine and faeces (p < 0.01). However, no significant difference was observed between capsule content and the liquid and solid parts of the fourth segment of the small intestine (p > 0.05). This study provides evidence that CapSa can effectively sample the intestinal microbiota of the middle part of the small intestine in growing pigs.http://dx.doi.org/10.1080/1828051X.2024.2427758intestinal microbiotasmall intestine samplingingestible capsule |
| spellingShingle | Inés García Viñado Federico Correa Paolo Trevisi Giuseppe Bee Catherine Ollagnier Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule Italian Journal of Animal Science intestinal microbiota small intestine sampling ingestible capsule |
| title | Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule |
| title_full | Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule |
| title_fullStr | Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule |
| title_full_unstemmed | Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule |
| title_short | Sampling intestinal microbiota in growing pigs: evaluation of CapSa, an ingestible capsule |
| title_sort | sampling intestinal microbiota in growing pigs evaluation of capsa an ingestible capsule |
| topic | intestinal microbiota small intestine sampling ingestible capsule |
| url | http://dx.doi.org/10.1080/1828051X.2024.2427758 |
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