ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK
Subject of research. Venous blood of patients with gastric cancer hospitalized with gastrointestinal bleeding. The study group included 14 men (45 %), 17 women (55 %), average age 63.2 ± 3.5 (M ± m). The comparison group included 58 patients undergoing medical examination prior to surgical treatment...
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| Format: | Article |
| Language: | Russian |
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The Publishing House Medicine and Enlightenment
2020-03-01
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| Series: | Медицина в Кузбассе |
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| Online Access: | http://mednauki.ru/index.php/MK/article/view/410 |
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| author | Анна Александровна Архипова Владимир Владимирович Анищенко |
| author_facet | Анна Александровна Архипова Владимир Владимирович Анищенко |
| author_sort | Анна Александровна Архипова |
| collection | DOAJ |
| description | Subject of research. Venous blood of patients with gastric cancer hospitalized with gastrointestinal bleeding. The study group included 14 men (45 %), 17 women (55 %), average age 63.2 ± 3.5 (M ± m). The comparison group included 58 patients undergoing medical examination prior to surgical treatment (replacement arthroplasty). Gender distribution of the patients in the comparison group – 19 men (33 %), 39 women (67 %), average age 62.4 ± 1.8 (M ± m)
Objective – to determine the risk of development of gastric cancer based on the analysis of polymorphism of tumor necrosis factor alpha.
Research methods. DNA isolation was performed using phenol-chloroform extraction. Genotyping of single nucleotide substitutions in the TNF-α gene at -308 (G; A) was performed using real-time PCR with competitive TaqMan probes complementary to polymorphic DNA regions. The accuracy of the genotyping is confirmed by sequencing.
Key findings. The distribution of TNF-α genotypes at -308 (G; A): GG 58 %, GA 42 % and AA 0 % in patients with gastric cancer and GG 79 %, GA 21 % and AA 0 % in the comparison group. In the group of patients with cancer the heterozygous GA genotype of the TNF-a gene was more frequent than in the comparison group: 42 % versus 21 % (χ2 = 4.514; р = 0,034). Calculation of odds ratio has shown that the GA genotype is associated with risk of gastric cancer development (OR = 2.769; lower limit 95% CI = 1.065, upper limit 95% CI = 7.197).
Scope of application. Formation of gastric cancer development risk groups.
Conclusions. The results of our study demonstrate the association between the heterozygous GA TNF-a-308 genotype and the risk of development of gastric cancer. |
| format | Article |
| id | doaj-art-da9ea744cb5348a980a51c9c9db3f8be |
| institution | Kabale University |
| issn | 1819-0901 2588-0411 |
| language | Russian |
| publishDate | 2020-03-01 |
| publisher | The Publishing House Medicine and Enlightenment |
| record_format | Article |
| series | Медицина в Кузбассе |
| spelling | doaj-art-da9ea744cb5348a980a51c9c9db3f8be2025-08-20T03:58:50ZrusThe Publishing House Medicine and EnlightenmentМедицина в Кузбассе1819-09012588-04112020-03-011914245388ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISKАнна Александровна Архипова0Владимир Владимирович Анищенко1ГБУЗ НСО ГКБ № 2, г. НовосибирскФГБОУ ВО НГМУ Минздрава России, г. НовосибирскSubject of research. Venous blood of patients with gastric cancer hospitalized with gastrointestinal bleeding. The study group included 14 men (45 %), 17 women (55 %), average age 63.2 ± 3.5 (M ± m). The comparison group included 58 patients undergoing medical examination prior to surgical treatment (replacement arthroplasty). Gender distribution of the patients in the comparison group – 19 men (33 %), 39 women (67 %), average age 62.4 ± 1.8 (M ± m) Objective – to determine the risk of development of gastric cancer based on the analysis of polymorphism of tumor necrosis factor alpha. Research methods. DNA isolation was performed using phenol-chloroform extraction. Genotyping of single nucleotide substitutions in the TNF-α gene at -308 (G; A) was performed using real-time PCR with competitive TaqMan probes complementary to polymorphic DNA regions. The accuracy of the genotyping is confirmed by sequencing. Key findings. The distribution of TNF-α genotypes at -308 (G; A): GG 58 %, GA 42 % and AA 0 % in patients with gastric cancer and GG 79 %, GA 21 % and AA 0 % in the comparison group. In the group of patients with cancer the heterozygous GA genotype of the TNF-a gene was more frequent than in the comparison group: 42 % versus 21 % (χ2 = 4.514; р = 0,034). Calculation of odds ratio has shown that the GA genotype is associated with risk of gastric cancer development (OR = 2.769; lower limit 95% CI = 1.065, upper limit 95% CI = 7.197). Scope of application. Formation of gastric cancer development risk groups. Conclusions. The results of our study demonstrate the association between the heterozygous GA TNF-a-308 genotype and the risk of development of gastric cancer.http://mednauki.ru/index.php/MK/article/view/410рак желудкакровотечениеполиморфизмрискфактор некроза опухоли |
| spellingShingle | Анна Александровна Архипова Владимир Владимирович Анищенко ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK Медицина в Кузбассе рак желудка кровотечение полиморфизм риск фактор некроза опухоли |
| title | ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK |
| title_full | ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK |
| title_fullStr | ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK |
| title_full_unstemmed | ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK |
| title_short | ASSOCIATION BETWEEN TNF-Α-308 G/A GENE POLYMORPHISM AND GASTRIC CANCER RISK |
| title_sort | association between tnf α 308 g a gene polymorphism and gastric cancer risk |
| topic | рак желудка кровотечение полиморфизм риск фактор некроза опухоли |
| url | http://mednauki.ru/index.php/MK/article/view/410 |
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