Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus
OBJECTIVE: This study investigated vimentin expression in placentas of patients with preeclampsia and gestational diabetes mellitus (GDM). STUDY DESIGN: Placentas of preeclamptic women (n=25), women with GDM (n=25), and control cases (n=25) were enrolled in this study. Placental samples were fixe...
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Medical Network
2024-04-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/1469 |
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| author | Fırat Asir Suleyman Cemil Oglak Tugcan Korak Fatih Tas Mehmet Yilmaz Fikri Erdemci Firat Sahin Hayat Ayaz Emine Zeynep Yilmaz Gokhan Bolluk |
| author_facet | Fırat Asir Suleyman Cemil Oglak Tugcan Korak Fatih Tas Mehmet Yilmaz Fikri Erdemci Firat Sahin Hayat Ayaz Emine Zeynep Yilmaz Gokhan Bolluk |
| author_sort | Fırat Asir |
| collection | DOAJ |
| description |
OBJECTIVE: This study investigated vimentin expression in placentas of patients with preeclampsia and gestational diabetes mellitus (GDM).
STUDY DESIGN: Placentas of preeclamptic women (n=25), women with GDM (n=25), and control cases (n=25) were enrolled in this study. Placental samples were fixed in zinc-formalin and further processed for paraffin wax tissue embedding. Demographic and laboratory parameters of patients were recorded. Vimentin immune activity was analyzed in the placental sections with immunohistochemistry. Sections were imaged and analyzed under a light microscope. A semiquantitative measurement was done between groups by comparing the Vimentin signal and significance was calculated. Network construction and pathway enrichment analysis were conducted using Cytoscape (v3.10.1) and ShinyGO, respectively.
RESULTS: Vimentin expression was high in the placental sections of the control group. The preeclampsia group showed positive Vimentin expression in cytotrophoblast and syncytiotrophoblast cells and connective tissue of placental villi in the preeclampsia group. Vimentin expression was generally recorded as negative in placental villi, fibrinoid substances, and connective tissue cells in the GDM group. Bioinformatic analysis showed that the AGE-RAGE signaling pathway and cancer-related pathways were mainly observed in Vimentin-associated pathways, which finally activate inflammatory pathways in both preeclampsia and GDM.
CONCLUSION: Vimentin expression patterns in placental tissue sections reveal nuanced regulatory mechanisms, emphasizing the need for further exploration into the functional roles of vimentin in placental physiology and pathology.
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| format | Article |
| id | doaj-art-da90ee4038c44265bc64aa793c5a37ec |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Medical Network |
| record_format | Article |
| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-da90ee4038c44265bc64aa793c5a37ec2025-02-11T21:18:14ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182024-04-0130110.21613/GORM.2023.1469Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus Fırat Asir0Suleyman Cemil Oglak1Tugcan Korak2Fatih Tas3Mehmet Yilmaz4Fikri Erdemci5Firat Sahin6Hayat Ayaz7Emine Zeynep Yilmaz8Gokhan Bolluk9Department of Histology and Embryology, Dicle University Faculty of Medicine, Diyarbakır, TurkeyUniversity of Health Sciences, Diyarbakır Gazi Yaşargil Training and Research Hospital, Department of Obstetrics and GynecologyDepartment of Medical Biology and Genetics, Koceali University Faculty of Medicine, Kocaeli, TurkeyDepartment of Histology and Embryology, Siirt University Faculty of Medicine, Siirt, TurkeyDepartment of Obstetrics and Gynecology, Mardin Artuklu University Faculty of Medicine, Mardin, TurkeyDepartment of Histology and Embryology, Dicle University Faculty of Medicine, Diyarbakır, TurkeyDepartment of Histology and Embryology, Dicle University Faculty of Medicine, Diyarbakır, TurkeyDepartment of Histology and Embryology, Dicle University Faculty of Medicine, Diyarbakır, TurkeyDepartment of Obstetrics and Gynecology, Bahçelievler Memorial Hospital, Istanbul, TurkeyDepartment of Perinatology, Health Sciences University, Başakşehir Çam and Sakura City Hospital, Istanbul, Turkey OBJECTIVE: This study investigated vimentin expression in placentas of patients with preeclampsia and gestational diabetes mellitus (GDM). STUDY DESIGN: Placentas of preeclamptic women (n=25), women with GDM (n=25), and control cases (n=25) were enrolled in this study. Placental samples were fixed in zinc-formalin and further processed for paraffin wax tissue embedding. Demographic and laboratory parameters of patients were recorded. Vimentin immune activity was analyzed in the placental sections with immunohistochemistry. Sections were imaged and analyzed under a light microscope. A semiquantitative measurement was done between groups by comparing the Vimentin signal and significance was calculated. Network construction and pathway enrichment analysis were conducted using Cytoscape (v3.10.1) and ShinyGO, respectively. RESULTS: Vimentin expression was high in the placental sections of the control group. The preeclampsia group showed positive Vimentin expression in cytotrophoblast and syncytiotrophoblast cells and connective tissue of placental villi in the preeclampsia group. Vimentin expression was generally recorded as negative in placental villi, fibrinoid substances, and connective tissue cells in the GDM group. Bioinformatic analysis showed that the AGE-RAGE signaling pathway and cancer-related pathways were mainly observed in Vimentin-associated pathways, which finally activate inflammatory pathways in both preeclampsia and GDM. CONCLUSION: Vimentin expression patterns in placental tissue sections reveal nuanced regulatory mechanisms, emphasizing the need for further exploration into the functional roles of vimentin in placental physiology and pathology. https://gorm.com.tr/index.php/GORM/article/view/1469Gestational diabetes mellitus Placenta Preeclampsiavimentin |
| spellingShingle | Fırat Asir Suleyman Cemil Oglak Tugcan Korak Fatih Tas Mehmet Yilmaz Fikri Erdemci Firat Sahin Hayat Ayaz Emine Zeynep Yilmaz Gokhan Bolluk Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus Gynecology Obstetrics & Reproductive Medicine Gestational diabetes mellitus Placenta Preeclampsia vimentin |
| title | Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus |
| title_full | Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus |
| title_fullStr | Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus |
| title_full_unstemmed | Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus |
| title_short | Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus |
| title_sort | placental vimentin expression in preeclampsia and gestational diabetes mellitus |
| topic | Gestational diabetes mellitus Placenta Preeclampsia vimentin |
| url | https://gorm.com.tr/index.php/GORM/article/view/1469 |
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