Evolutionary origins and functional diversification of Auxin Response Factors
Abstract The Auxin Response Factors (ARFs) family of transcription factors are the central mediators of auxin-triggered transcriptional regulation. Functionally different classes of extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part of the evolutionary trajec...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55278-8 |
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| author | Jorge Hernández-García Vanessa Polet Carrillo-Carrasco Juriaan Rienstra Keita Tanaka Martijn de Roij Melissa Dipp-Álvarez Alejandra Freire-Ríos Isidro Crespo Roeland Boer Willy A. M. van den Berg Simon Lindhoud Dolf Weijers |
| author_facet | Jorge Hernández-García Vanessa Polet Carrillo-Carrasco Juriaan Rienstra Keita Tanaka Martijn de Roij Melissa Dipp-Álvarez Alejandra Freire-Ríos Isidro Crespo Roeland Boer Willy A. M. van den Berg Simon Lindhoud Dolf Weijers |
| author_sort | Jorge Hernández-García |
| collection | DOAJ |
| description | Abstract The Auxin Response Factors (ARFs) family of transcription factors are the central mediators of auxin-triggered transcriptional regulation. Functionally different classes of extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part of the evolutionary trajectory to the present auxin response functions has been reconstructed, it is unclear how ARFs emerged, and how early diversification led to functionally different proteins. Here, we use in silico and in vivo analyses to revisit the molecular events that led to the origin and subsequent evolution of the ARFs. We reveal the shared origin of ARFs from preexisting domains, uncovering a protein fold homologous to the ARF DNA-binding fold in a conserved eukaryotic chromatin regulator. Building on this, we reconstruct the complete evolutionary history of ARFs, including the divergence events leading to the appearance of the ARF classes and defining the main molecular targets for their functional diversification. We derive a complete evolutionary trajectory that led to the emergence of the nuclear auxin signalling pathway. |
| format | Article |
| id | doaj-art-da834b32010f4448a1a95a232cc9317d |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-da834b32010f4448a1a95a232cc9317d2025-08-20T03:43:11ZengNature PortfolioNature Communications2041-17232024-12-0115111610.1038/s41467-024-55278-8Evolutionary origins and functional diversification of Auxin Response FactorsJorge Hernández-García0Vanessa Polet Carrillo-Carrasco1Juriaan Rienstra2Keita Tanaka3Martijn de Roij4Melissa Dipp-Álvarez5Alejandra Freire-Ríos6Isidro Crespo7Roeland Boer8Willy A. M. van den Berg9Simon Lindhoud10Dolf Weijers11Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Experiments Division, ALBA Synchrotron Light Source, Carrer de la Llum 2–26Experiments Division, ALBA Synchrotron Light Source, Carrer de la Llum 2–26Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Laboratory of Biochemistry, Wageningen University, Stippeneng 4Abstract The Auxin Response Factors (ARFs) family of transcription factors are the central mediators of auxin-triggered transcriptional regulation. Functionally different classes of extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part of the evolutionary trajectory to the present auxin response functions has been reconstructed, it is unclear how ARFs emerged, and how early diversification led to functionally different proteins. Here, we use in silico and in vivo analyses to revisit the molecular events that led to the origin and subsequent evolution of the ARFs. We reveal the shared origin of ARFs from preexisting domains, uncovering a protein fold homologous to the ARF DNA-binding fold in a conserved eukaryotic chromatin regulator. Building on this, we reconstruct the complete evolutionary history of ARFs, including the divergence events leading to the appearance of the ARF classes and defining the main molecular targets for their functional diversification. We derive a complete evolutionary trajectory that led to the emergence of the nuclear auxin signalling pathway.https://doi.org/10.1038/s41467-024-55278-8 |
| spellingShingle | Jorge Hernández-García Vanessa Polet Carrillo-Carrasco Juriaan Rienstra Keita Tanaka Martijn de Roij Melissa Dipp-Álvarez Alejandra Freire-Ríos Isidro Crespo Roeland Boer Willy A. M. van den Berg Simon Lindhoud Dolf Weijers Evolutionary origins and functional diversification of Auxin Response Factors Nature Communications |
| title | Evolutionary origins and functional diversification of Auxin Response Factors |
| title_full | Evolutionary origins and functional diversification of Auxin Response Factors |
| title_fullStr | Evolutionary origins and functional diversification of Auxin Response Factors |
| title_full_unstemmed | Evolutionary origins and functional diversification of Auxin Response Factors |
| title_short | Evolutionary origins and functional diversification of Auxin Response Factors |
| title_sort | evolutionary origins and functional diversification of auxin response factors |
| url | https://doi.org/10.1038/s41467-024-55278-8 |
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