Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi.
Agarwood is a precious resinous heartwood highly valued for its cultural, religious, and medicinal significance. With the increasing market demand, natural agarwood resources are rapidly depleting, making the development of effective artificial induction methods for agarwood highly significant. This...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0316178 |
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| author | Zhikai Wang Guoying Zhou Jungang Chen Xinyu Miao Yandong Xia Zhuang Du Junang Liu |
| author_facet | Zhikai Wang Guoying Zhou Jungang Chen Xinyu Miao Yandong Xia Zhuang Du Junang Liu |
| author_sort | Zhikai Wang |
| collection | DOAJ |
| description | Agarwood is a precious resinous heartwood highly valued for its cultural, religious, and medicinal significance. With the increasing market demand, natural agarwood resources are rapidly depleting, making the development of effective artificial induction methods for agarwood highly significant. This study aims to explore the feasibility of using callus tissue to assess the ability of fungi to induce agarwood formation. We selected two fungi isolated from Aquilaria sinensis, W-1 (Podospora setosa) and W-15 (Alternaria alstroemeriae), and used the known agarwood-inducing fungi YMY (Pestalotiopsis sp.) as a positive control, by treating A. sinensis callus with their fermented filtrates. The experimental results showed that W-1 and W-15 treatments significantly enhanced the activity of Superoxide dismutase (SOD) and Peroxidase (POD) in the callus tissue and upregulated the expression of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), and sesquiterpene synthase (ASS-1). GC-MS analysis further confirmed that the contents of sesquiterpenes and aromatic compounds in A. sinensis treated with W-1 and W-15 were significantly elevated, suggesting that these fungi possess the capacity to induce the formation of agarwood. This study demonstrates that using callus tissue to screen fungi capable of inducing agarwood is feasible and effective, providing new insights for screening fungi resources that efficiently induce agarwood formation in the future. |
| format | Article |
| id | doaj-art-da7edfbaf45646648beea3e2e42cf08b |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-da7edfbaf45646648beea3e2e42cf08b2025-01-08T05:32:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031617810.1371/journal.pone.0316178Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi.Zhikai WangGuoying ZhouJungang ChenXinyu MiaoYandong XiaZhuang DuJunang LiuAgarwood is a precious resinous heartwood highly valued for its cultural, religious, and medicinal significance. With the increasing market demand, natural agarwood resources are rapidly depleting, making the development of effective artificial induction methods for agarwood highly significant. This study aims to explore the feasibility of using callus tissue to assess the ability of fungi to induce agarwood formation. We selected two fungi isolated from Aquilaria sinensis, W-1 (Podospora setosa) and W-15 (Alternaria alstroemeriae), and used the known agarwood-inducing fungi YMY (Pestalotiopsis sp.) as a positive control, by treating A. sinensis callus with their fermented filtrates. The experimental results showed that W-1 and W-15 treatments significantly enhanced the activity of Superoxide dismutase (SOD) and Peroxidase (POD) in the callus tissue and upregulated the expression of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), and sesquiterpene synthase (ASS-1). GC-MS analysis further confirmed that the contents of sesquiterpenes and aromatic compounds in A. sinensis treated with W-1 and W-15 were significantly elevated, suggesting that these fungi possess the capacity to induce the formation of agarwood. This study demonstrates that using callus tissue to screen fungi capable of inducing agarwood is feasible and effective, providing new insights for screening fungi resources that efficiently induce agarwood formation in the future.https://doi.org/10.1371/journal.pone.0316178 |
| spellingShingle | Zhikai Wang Guoying Zhou Jungang Chen Xinyu Miao Yandong Xia Zhuang Du Junang Liu Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. PLoS ONE |
| title | Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. |
| title_full | Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. |
| title_fullStr | Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. |
| title_full_unstemmed | Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. |
| title_short | Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi. |
| title_sort | research on using aquilaria sinensis callus to evaluate the agarwood inducing potential of fungi |
| url | https://doi.org/10.1371/journal.pone.0316178 |
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