Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism
Flos Chrysanthemi Indici (FCI) is a Chinese herbal medicine used in China for over 2000 years. In this study, its ethanol extract (EFCL) was found to protect against hydroxyl radical (·−OH) induced oxidative damages to mesenchymal stem cells (MSCs) and DNA. To explore the mechanism, EFCL was further...
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Springer
2015-07-01
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| Series: | Journal of Saudi Chemical Society |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S131961031400088X |
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| author | Xican Li Qiuping Hu Shuxia Jiang Fei Li Jian Lin Lu Han Yuling Hong Wenbiao Lu Yaoxiang Gao Dongfeng Chen |
| author_facet | Xican Li Qiuping Hu Shuxia Jiang Fei Li Jian Lin Lu Han Yuling Hong Wenbiao Lu Yaoxiang Gao Dongfeng Chen |
| author_sort | Xican Li |
| collection | DOAJ |
| description | Flos Chrysanthemi Indici (FCI) is a Chinese herbal medicine used in China for over 2000 years. In this study, its ethanol extract (EFCL) was found to protect against hydroxyl radical (·−OH) induced oxidative damages to mesenchymal stem cells (MSCs) and DNA. To explore the mechanism, EFCL was further determined by chemical and antioxidant assays. Folin–Ciocalteu colorimetric assay suggested that content of total phenolics was 93.85 ± 3.56 mg catechin/g and HPLC analysis indicated a content of 10.05 ± 1.44 mg/g as chlorogenic acid in EFCI. Antioxidant assays revealed that EFCI could also scavenge ·−O2− radical (IC50 58.74 ± 1.30 μg/mL), DPPH·− radical (IC50 46.46 ± 1.35 μg/mL) and ABTS+·− radical (IC50 20.59 ± 0.52 μg/mL), bind Fe2+ (IC50 364.27 ± 19.57 μg/mL) and Cu2+ (IC50 734.77 ± 34.69 μg/mL), reduce Fe3+ (IC50 147.11 ± 11.09 μg/mL) and Cu2+ (IC50 35.69 ± 3.14 μg/mL). On the basis of mechanistic analysis, we concluded that FCI can effectively protect against hydroxyl-induced damages to DNA and MSCs, therefore FCI shows promise as a possible therapeutic reagent for many diseases. The effect may be mainly attributed to phenolics especially chlorogenic acid, which exerts the antioxidant action possibly through metal-chelating, and radical-scavenging which is via hydrogen atom transfer (HAT) and/or sequential electron proton transfer (SEPT) mechanisms. |
| format | Article |
| id | doaj-art-da7e7b53cd36409db9e63ff178eb3add |
| institution | Kabale University |
| issn | 1319-6103 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj-art-da7e7b53cd36409db9e63ff178eb3add2025-08-20T03:52:07ZengSpringerJournal of Saudi Chemical Society1319-61032015-07-0119445446010.1016/j.jscs.2014.06.004Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanismXican Li0Qiuping Hu1Shuxia Jiang2Fei Li3Jian Lin4Lu Han5Yuling Hong6Wenbiao Lu7Yaoxiang Gao8Dongfeng Chen9School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaFlos Chrysanthemi Indici (FCI) is a Chinese herbal medicine used in China for over 2000 years. In this study, its ethanol extract (EFCL) was found to protect against hydroxyl radical (·−OH) induced oxidative damages to mesenchymal stem cells (MSCs) and DNA. To explore the mechanism, EFCL was further determined by chemical and antioxidant assays. Folin–Ciocalteu colorimetric assay suggested that content of total phenolics was 93.85 ± 3.56 mg catechin/g and HPLC analysis indicated a content of 10.05 ± 1.44 mg/g as chlorogenic acid in EFCI. Antioxidant assays revealed that EFCI could also scavenge ·−O2− radical (IC50 58.74 ± 1.30 μg/mL), DPPH·− radical (IC50 46.46 ± 1.35 μg/mL) and ABTS+·− radical (IC50 20.59 ± 0.52 μg/mL), bind Fe2+ (IC50 364.27 ± 19.57 μg/mL) and Cu2+ (IC50 734.77 ± 34.69 μg/mL), reduce Fe3+ (IC50 147.11 ± 11.09 μg/mL) and Cu2+ (IC50 35.69 ± 3.14 μg/mL). On the basis of mechanistic analysis, we concluded that FCI can effectively protect against hydroxyl-induced damages to DNA and MSCs, therefore FCI shows promise as a possible therapeutic reagent for many diseases. The effect may be mainly attributed to phenolics especially chlorogenic acid, which exerts the antioxidant action possibly through metal-chelating, and radical-scavenging which is via hydrogen atom transfer (HAT) and/or sequential electron proton transfer (SEPT) mechanisms.http://www.sciencedirect.com/science/article/pii/S131961031400088XFlos Chrysanthemi IndiciHydroxyl-induced damageAntioxidant MechanismHydrogen atom transfer HATSequential electron proton transfer SEPTChlorogenic acidTotal phenolics |
| spellingShingle | Xican Li Qiuping Hu Shuxia Jiang Fei Li Jian Lin Lu Han Yuling Hong Wenbiao Lu Yaoxiang Gao Dongfeng Chen Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism Journal of Saudi Chemical Society Flos Chrysanthemi Indici Hydroxyl-induced damage Antioxidant Mechanism Hydrogen atom transfer HAT Sequential electron proton transfer SEPT Chlorogenic acid Total phenolics |
| title | Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism |
| title_full | Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism |
| title_fullStr | Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism |
| title_full_unstemmed | Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism |
| title_short | Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism |
| title_sort | flos chrysanthemi indici protects against hydroxyl induced damages to dna and mscs via antioxidant mechanism |
| topic | Flos Chrysanthemi Indici Hydroxyl-induced damage Antioxidant Mechanism Hydrogen atom transfer HAT Sequential electron proton transfer SEPT Chlorogenic acid Total phenolics |
| url | http://www.sciencedirect.com/science/article/pii/S131961031400088X |
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