Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes
Background. Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation. Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling. In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/823279 |
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| author | Esther López M. Paz Zafra Beatriz Sastre Cristina Gámez Carlos Lahoz Victoria del Pozo |
| author_facet | Esther López M. Paz Zafra Beatriz Sastre Cristina Gámez Carlos Lahoz Victoria del Pozo |
| author_sort | Esther López |
| collection | DOAJ |
| description | Background. Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation. Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling. In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the balance between Th1 and Th2 cells. In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation. Methods. Using a microarray approach, this study endeavored to evaluate the changes produced by therapeutic Gal-3 delivered by gene therapy in a well-characterized mouse model of chronic airway inflammation. Results were confirmed by real-time RT-PCR, Western blot and immunohistochemical analysis. Results. We identify a set of genes involved in different pathways whose expression is coordinately decreased/increased in mice treated with Gal-3 gene therapy. We report a correlation between Gal-3 treatment and inhibition of SOCS1 and SOCS3 expression in lungs. Conclusion. These results suggest that negative regulation of SOCS1 and 3 following Gal-3 treatment could be a valuable therapeutic approach in allergic disease. |
| format | Article |
| id | doaj-art-da7a0a41213647908efd8b9da1de39bb |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-da7a0a41213647908efd8b9da1de39bb2025-02-03T05:48:11ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/823279823279Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory GenesEsther López0M. Paz Zafra1Beatriz Sastre2Cristina Gámez3Carlos Lahoz4Victoria del Pozo5Immunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainImmunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainImmunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainImmunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainImmunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainImmunology Department IIS-Fundación Jiménez Díaz, CIBER de Enfermedades Respiratorias (CIBERES), 28040 Madrid, SpainBackground. Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation. Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling. In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the balance between Th1 and Th2 cells. In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation. Methods. Using a microarray approach, this study endeavored to evaluate the changes produced by therapeutic Gal-3 delivered by gene therapy in a well-characterized mouse model of chronic airway inflammation. Results were confirmed by real-time RT-PCR, Western blot and immunohistochemical analysis. Results. We identify a set of genes involved in different pathways whose expression is coordinately decreased/increased in mice treated with Gal-3 gene therapy. We report a correlation between Gal-3 treatment and inhibition of SOCS1 and SOCS3 expression in lungs. Conclusion. These results suggest that negative regulation of SOCS1 and 3 following Gal-3 treatment could be a valuable therapeutic approach in allergic disease.http://dx.doi.org/10.1155/2011/823279 |
| spellingShingle | Esther López M. Paz Zafra Beatriz Sastre Cristina Gámez Carlos Lahoz Victoria del Pozo Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes Mediators of Inflammation |
| title | Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes |
| title_full | Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes |
| title_fullStr | Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes |
| title_full_unstemmed | Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes |
| title_short | Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes |
| title_sort | gene expression profiling in lungs of chronic asthmatic mice treated with galectin 3 downregulation of inflammatory and regulatory genes |
| url | http://dx.doi.org/10.1155/2011/823279 |
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