Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis
In this study, we present a promising approach for the rapid development of porous polydimethylsiloxane (PDMS) scaffold prototypes, with outer geometry defined from the design stage, according to the form of conventional implants or adapted to patients’ biostructures. The manufacture method is based...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2014/612976 |
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| author | Andrés Díaz Lantada Hernán Alarcón Iniesta Beatriz Pareja Sánchez Josefa Predestinación García-Ruíz |
| author_facet | Andrés Díaz Lantada Hernán Alarcón Iniesta Beatriz Pareja Sánchez Josefa Predestinación García-Ruíz |
| author_sort | Andrés Díaz Lantada |
| collection | DOAJ |
| description | In this study, we present a promising approach for the rapid development of porous polydimethylsiloxane (PDMS) scaffold prototypes, with outer geometry defined from the design stage, according to the form of conventional implants or adapted to patients’ biostructures. The manufacture method is based on phase separation processes using materials obtained by casting within additive rapid prototyped molds. We include a comparative study of PDMS sponges obtained by different simple processes. Final in vitro assessment is carried out using hMSCs (bone marrow-derived human mesenchymal stem cells), cultured onto porous PDMS scaffolds functionalized with aminopropyltriethoxysilane (APTS) and equilibrated with a trophic factors medium produced by the cells. Results show that porous PDMS scaffold prototypes are excellent 3D platforms for hMSCs adhesion. Furthermore, this PDMS-3D niche, seeded with hMSCs and chondrogenic incubation medium during three weeks, showed a successful chondrogenesis determined by collagen type II expression. Thus, results show a versatile method to produce a 3D niche to address questions about cartilage and endochondral bone formation or skeleton tissues clinical approaches. |
| format | Article |
| id | doaj-art-da6f46ab7bb043bcb6ab27c8f578024e |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-da6f46ab7bb043bcb6ab27c8f578024e2025-08-20T03:24:25ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422014-01-01201410.1155/2014/612976612976Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote ChondrogenesisAndrés Díaz Lantada0Hernán Alarcón Iniesta1Beatriz Pareja Sánchez2Josefa Predestinación García-Ruíz3Product Development Laboratory, Mechanical Engineering & Manufacturing Department, Universidad Politécnica de Madrid (UPM), c/José Gutiérrez Abascal 2, 28006 Madrid, SpainDepartamento de Biología Molecular, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, SpainProduct Development Laboratory, Mechanical Engineering & Manufacturing Department, Universidad Politécnica de Madrid (UPM), c/José Gutiérrez Abascal 2, 28006 Madrid, SpainDepartamento de Biología Molecular, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, SpainIn this study, we present a promising approach for the rapid development of porous polydimethylsiloxane (PDMS) scaffold prototypes, with outer geometry defined from the design stage, according to the form of conventional implants or adapted to patients’ biostructures. The manufacture method is based on phase separation processes using materials obtained by casting within additive rapid prototyped molds. We include a comparative study of PDMS sponges obtained by different simple processes. Final in vitro assessment is carried out using hMSCs (bone marrow-derived human mesenchymal stem cells), cultured onto porous PDMS scaffolds functionalized with aminopropyltriethoxysilane (APTS) and equilibrated with a trophic factors medium produced by the cells. Results show that porous PDMS scaffold prototypes are excellent 3D platforms for hMSCs adhesion. Furthermore, this PDMS-3D niche, seeded with hMSCs and chondrogenic incubation medium during three weeks, showed a successful chondrogenesis determined by collagen type II expression. Thus, results show a versatile method to produce a 3D niche to address questions about cartilage and endochondral bone formation or skeleton tissues clinical approaches.http://dx.doi.org/10.1155/2014/612976 |
| spellingShingle | Andrés Díaz Lantada Hernán Alarcón Iniesta Beatriz Pareja Sánchez Josefa Predestinación García-Ruíz Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis Advances in Materials Science and Engineering |
| title | Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis |
| title_full | Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis |
| title_fullStr | Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis |
| title_full_unstemmed | Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis |
| title_short | Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis |
| title_sort | free form rapid prototyped porous pdms scaffolds incorporating growth factors promote chondrogenesis |
| url | http://dx.doi.org/10.1155/2014/612976 |
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