Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance
Abstract Bacillus pumilus plays an essential role in agricultural applications as a beneficial microbe and for sustainable agriculture production. However, the underlying mechanisms of B. pumilus strains remain unclear as to how they are beneficial for plants as stress tolerant and growth promoters....
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-78227-3 |
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| author | Shifa Shaffique Anis Ali Shah Peter Odongkara Hosam O. Elansary Abdul Latif Khan Arjun Adhikari Sang-Mo Kang In-Jung Lee |
| author_facet | Shifa Shaffique Anis Ali Shah Peter Odongkara Hosam O. Elansary Abdul Latif Khan Arjun Adhikari Sang-Mo Kang In-Jung Lee |
| author_sort | Shifa Shaffique |
| collection | DOAJ |
| description | Abstract Bacillus pumilus plays an essential role in agricultural applications as a beneficial microbe and for sustainable agriculture production. However, the underlying mechanisms of B. pumilus strains remain unclear as to how they are beneficial for plants as stress tolerant and growth promoters. Bacillus pumilus was isolated from the rhizosphere soil of Artemisia vulgaris. NGS (next-generation sequencing) was performed for the strain to gain new insights into the molecular mechanisms underlying plant-microbial interactions. NGS revealed 3,910 genes, 3294 genes with protein-coding, and 11 functional genomic regions related to diverse agronomic traits including stress tolerance. We identified the two possible phytohormone biosynthesis approaches from metabolic regions1(terpense→diterpense→betacarotene→xanthoxin→ABA)2(terpense→diterpense→geranyl diphosphate →C20 →GA). Several gene clusters related to the biosynthesis of phytohormones, stress tolerance, and agricultural diversification were predicted. The genome provides insights into the possible mechanisms of this bacterium for stress tolerance and its future applications. The genomic organization of B. pumilus revealed several hallmarks of its plant growth promotion and pathogen suppression activities. Our results provide detailed genomic information for the strain and reveal its potential stress tolerance mechanisms, laying the foundation for developing effective stress tolerance strategies against abiotic stress. |
| format | Article |
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| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-da6924ae014e405eb37c3703ec3e4ddf2025-08-20T02:31:51ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-78227-3Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress toleranceShifa Shaffique0Anis Ali Shah1Peter Odongkara2Hosam O. Elansary3Abdul Latif Khan4Arjun Adhikari5Sang-Mo Kang6In-Jung Lee7School of Applied Biosciences, Kyungpook National UniversityDepartment of Botany, Division of Science and Technology, University of EducationSchool of Applied Biosciences, Kyungpook National UniversityPrince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud UniversityDepartment of Biology and Biochemistry, University of HoustonSchool of Applied Biosciences, Kyungpook National UniversitySchool of Applied Biosciences, Kyungpook National UniversitySchool of Applied Biosciences, Kyungpook National UniversityAbstract Bacillus pumilus plays an essential role in agricultural applications as a beneficial microbe and for sustainable agriculture production. However, the underlying mechanisms of B. pumilus strains remain unclear as to how they are beneficial for plants as stress tolerant and growth promoters. Bacillus pumilus was isolated from the rhizosphere soil of Artemisia vulgaris. NGS (next-generation sequencing) was performed for the strain to gain new insights into the molecular mechanisms underlying plant-microbial interactions. NGS revealed 3,910 genes, 3294 genes with protein-coding, and 11 functional genomic regions related to diverse agronomic traits including stress tolerance. We identified the two possible phytohormone biosynthesis approaches from metabolic regions1(terpense→diterpense→betacarotene→xanthoxin→ABA)2(terpense→diterpense→geranyl diphosphate →C20 →GA). Several gene clusters related to the biosynthesis of phytohormones, stress tolerance, and agricultural diversification were predicted. The genome provides insights into the possible mechanisms of this bacterium for stress tolerance and its future applications. The genomic organization of B. pumilus revealed several hallmarks of its plant growth promotion and pathogen suppression activities. Our results provide detailed genomic information for the strain and reveal its potential stress tolerance mechanisms, laying the foundation for developing effective stress tolerance strategies against abiotic stress.https://doi.org/10.1038/s41598-024-78227-3Bacillus pumilusNGSFood securitySecondary metabolites |
| spellingShingle | Shifa Shaffique Anis Ali Shah Peter Odongkara Hosam O. Elansary Abdul Latif Khan Arjun Adhikari Sang-Mo Kang In-Jung Lee Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance Scientific Reports Bacillus pumilus NGS Food security Secondary metabolites |
| title | Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance |
| title_full | Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance |
| title_fullStr | Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance |
| title_full_unstemmed | Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance |
| title_short | Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance |
| title_sort | deciphering the aba and ga biosynthesis approach of bacillus pumilus mechanistic approach explaining the role of metabolic region as an aid in improving the stress tolerance |
| topic | Bacillus pumilus NGS Food security Secondary metabolites |
| url | https://doi.org/10.1038/s41598-024-78227-3 |
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