Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.

Background and objective: Programmed death ligand-1 protein expression has been linked to the severity of Urothelial cancer. The objectives of this study were to evaluate the expression of programmed death-ligand 1 and determine the association with tumour grade, stage, type (Papillary and non papi...

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Main Authors: Zuzan Shawkat Mustafa, Gina James Keorges, Alaa Hani Raziq
Format: Article
Language:English
Published: Kurdistan Higher Council Of Medical Specialties 2024-06-01
Series:Advanced Medical Journal
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Online Access:https://amj.khcms.edu.krd/index.php/main/article/view/416
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author Zuzan Shawkat Mustafa
Gina James Keorges
Alaa Hani Raziq
author_facet Zuzan Shawkat Mustafa
Gina James Keorges
Alaa Hani Raziq
author_sort Zuzan Shawkat Mustafa
collection DOAJ
description Background and objective: Programmed death ligand-1 protein expression has been linked to the severity of Urothelial cancer. The objectives of this study were to evaluate the expression of programmed death-ligand 1 and determine the association with tumour grade, stage, type (Papillary and non papillary), muscle invasion, age, and gender. Methods: This retrospective study was carried out from 2015 to 2022 on fifty-eight blocks belonging to patients with urothelial carcinoma, which were obtained from Duhok central lab and private laboratory. From each block, two sections were made; one of which was stained with Hematoxylin and Eosin for histological re-assessment and the other one for immunostaining for programmed death-ligand-1, which  was calculated in tumour cells and inflammatory cells regardless of staining intensity and then the combined positive score was determined and considered positive when is more than 10. Result: This study included 58 cases of Urothelial carcinoma, Females represent 8 (13.79%)  cases and males 50 (86.21%) cases, with a female-to-male ratio of 1/6.25. No statistically significant association existed between the histological subtypes and gender. Combined positive score was positive  in (32.76%) of cases and negative (67.24%) cases. The association was significant with the stage of the tumor (p=0.03), muscle invasion (p=0.02) and highly significant with grade (p<0.001). Conclusion: patients with urothelial carcinoma who have higher tumour grade, advanced stage and those who with muscle invasion can benefit from immune check point inhibitors.
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spelling doaj-art-da636099eb5349bcbb0d2fe43e4bb1a12025-08-20T02:17:33ZengKurdistan Higher Council Of Medical SpecialtiesAdvanced Medical Journal2958-89792957-35802024-06-019210.56056/amj.2024.257Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.Zuzan Shawkat Mustafa 0Gina James Keorges1Alaa Hani Raziq2Department of Histopathology, Central Lab, Duhok. FICMS. Lecturer - Department of Pathology, College of Medicine, University of DuhokFICMS. Professor - Department of Pathology, College of Medicine, University of Duhok Background and objective: Programmed death ligand-1 protein expression has been linked to the severity of Urothelial cancer. The objectives of this study were to evaluate the expression of programmed death-ligand 1 and determine the association with tumour grade, stage, type (Papillary and non papillary), muscle invasion, age, and gender. Methods: This retrospective study was carried out from 2015 to 2022 on fifty-eight blocks belonging to patients with urothelial carcinoma, which were obtained from Duhok central lab and private laboratory. From each block, two sections were made; one of which was stained with Hematoxylin and Eosin for histological re-assessment and the other one for immunostaining for programmed death-ligand-1, which  was calculated in tumour cells and inflammatory cells regardless of staining intensity and then the combined positive score was determined and considered positive when is more than 10. Result: This study included 58 cases of Urothelial carcinoma, Females represent 8 (13.79%)  cases and males 50 (86.21%) cases, with a female-to-male ratio of 1/6.25. No statistically significant association existed between the histological subtypes and gender. Combined positive score was positive  in (32.76%) of cases and negative (67.24%) cases. The association was significant with the stage of the tumor (p=0.03), muscle invasion (p=0.02) and highly significant with grade (p<0.001). Conclusion: patients with urothelial carcinoma who have higher tumour grade, advanced stage and those who with muscle invasion can benefit from immune check point inhibitors. https://amj.khcms.edu.krd/index.php/main/article/view/416Combined positive scorePDL-1Urothelial cancer
spellingShingle Zuzan Shawkat Mustafa
Gina James Keorges
Alaa Hani Raziq
Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
Advanced Medical Journal
Combined positive score
PDL-1
Urothelial cancer
title Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
title_full Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
title_fullStr Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
title_full_unstemmed Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
title_short Programmed Death Ligand 1 expression in Urothelial Carcinoma.A retrospective study in Duhok City-Iraq.
title_sort programmed death ligand 1 expression in urothelial carcinoma a retrospective study in duhok city iraq
topic Combined positive score
PDL-1
Urothelial cancer
url https://amj.khcms.edu.krd/index.php/main/article/view/416
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