Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification
The development of efficient methods for sensing αlpha-glucosidase (α-Glu) and screening its inhibitors has attracted significant attention due to their pivotal role in discovering therapeutic medicines for Type 2 diabetes. Herein, a low-cost and sensitive fluorometric strategy based on red carbon d...
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Elsevier
2025-08-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425005885 |
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| author | Huihui Sun Chuanyuan Gao Yumin Yang Changqing Liu Han Qin Mengyuan Tan Jin Li Xiaoxia Li Kunze Du Yanxu Chang |
| author_facet | Huihui Sun Chuanyuan Gao Yumin Yang Changqing Liu Han Qin Mengyuan Tan Jin Li Xiaoxia Li Kunze Du Yanxu Chang |
| author_sort | Huihui Sun |
| collection | DOAJ |
| description | The development of efficient methods for sensing αlpha-glucosidase (α-Glu) and screening its inhibitors has attracted significant attention due to their pivotal role in discovering therapeutic medicines for Type 2 diabetes. Herein, a low-cost and sensitive fluorometric strategy based on red carbon dots (R-CDs) and cobalt oxyhydroxide nanosheets (CoOOH NSs) had been established to detect α-Glu and screen its inhibitory compounds in natural products. As a switched fluorescence source, the fluorescence of R-CDs at 625 nm could be quenched by CoOOH NSs via Förster resonance energy transfer (FRET), assembled into nonfluorescent R-CDs@CoOOH nanocompositecomposites (R-CDs@CoOOH NCs). α-Glu hydrolyzed L-ascorbic acid-2-O-α-D-glucopyranose to produce ascorbic acid, which could reduce CoOOH NSs to Co2+, destroying R-CDs@CoOOH NCs and restoring the emission of red fluorescence. The proposed method exhibited a linear α-Glu range from 0.01 to 15 U mL−1 and a low limit of detection (LOD) of 0.0037 U mL−1. Meanwhile, high-performance liquid chromatography-DAD-fraction collector (HPLC-DAD-FC) had been employed and combined with ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry to isolate, enrich, and characterize compounds from Polygonum cuspidatum (PC). This strategy was further extended by integrating the fluorometric platform with the HPLC-DAD-FC system to explore the inhibitory effects of PC extracts and anti-diabetic ingredients. Finally, 85 constituents were identified, with seven compounds exhibiting high α-Glu inhibitory activity. Consequently, the established strategy could accurately determine α-Glu in vitro and screen its inhibitors from natural products. |
| format | Article |
| id | doaj-art-da6096760bc246cda55c7e4ee14d82f1 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-da6096760bc246cda55c7e4ee14d82f12025-08-20T03:27:02ZengElsevierMaterials Today Bio2590-00642025-08-013310201810.1016/j.mtbio.2025.102018Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identificationHuihui Sun0Chuanyuan Gao1Yumin Yang2Changqing Liu3Han Qin4Mengyuan Tan5Jin Li6Xiaoxia Li7Kunze Du8Yanxu Chang9State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaSchool of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Corresponding author. State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Corresponding author. State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.The development of efficient methods for sensing αlpha-glucosidase (α-Glu) and screening its inhibitors has attracted significant attention due to their pivotal role in discovering therapeutic medicines for Type 2 diabetes. Herein, a low-cost and sensitive fluorometric strategy based on red carbon dots (R-CDs) and cobalt oxyhydroxide nanosheets (CoOOH NSs) had been established to detect α-Glu and screen its inhibitory compounds in natural products. As a switched fluorescence source, the fluorescence of R-CDs at 625 nm could be quenched by CoOOH NSs via Förster resonance energy transfer (FRET), assembled into nonfluorescent R-CDs@CoOOH nanocompositecomposites (R-CDs@CoOOH NCs). α-Glu hydrolyzed L-ascorbic acid-2-O-α-D-glucopyranose to produce ascorbic acid, which could reduce CoOOH NSs to Co2+, destroying R-CDs@CoOOH NCs and restoring the emission of red fluorescence. The proposed method exhibited a linear α-Glu range from 0.01 to 15 U mL−1 and a low limit of detection (LOD) of 0.0037 U mL−1. Meanwhile, high-performance liquid chromatography-DAD-fraction collector (HPLC-DAD-FC) had been employed and combined with ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry to isolate, enrich, and characterize compounds from Polygonum cuspidatum (PC). This strategy was further extended by integrating the fluorometric platform with the HPLC-DAD-FC system to explore the inhibitory effects of PC extracts and anti-diabetic ingredients. Finally, 85 constituents were identified, with seven compounds exhibiting high α-Glu inhibitory activity. Consequently, the established strategy could accurately determine α-Glu in vitro and screen its inhibitors from natural products.http://www.sciencedirect.com/science/article/pii/S2590006425005885α-GlucosidaseFörster resonance energy transferInhibitorsPolygonum cuspidatumR-CDs@CoOOH NCsType 2 diabetes |
| spellingShingle | Huihui Sun Chuanyuan Gao Yumin Yang Changqing Liu Han Qin Mengyuan Tan Jin Li Xiaoxia Li Kunze Du Yanxu Chang Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification Materials Today Bio α-Glucosidase Förster resonance energy transfer Inhibitors Polygonum cuspidatum R-CDs@CoOOH NCs Type 2 diabetes |
| title | Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification |
| title_full | Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification |
| title_fullStr | Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification |
| title_full_unstemmed | Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification |
| title_short | Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification |
| title_sort | red emissive carbon dot cobalt oxyhydroxide nanosystem a turn on sensor for α glucosidase activity and inhibitor identification |
| topic | α-Glucosidase Förster resonance energy transfer Inhibitors Polygonum cuspidatum R-CDs@CoOOH NCs Type 2 diabetes |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425005885 |
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