Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China
Abstract Sorghum (Sorghum bicolor) is an important food and feed crop. Root-lesion nematodes (Pratylenchus spp.) are a group of pathogenic nematodes that cause severe economic losses in various food and cash crops. This study identified diseased sorghum plants with stunted growth and brown, rotting...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12866-025-03759-1 |
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| author | Ling Qin Fan-kang Lin Yun-long Lv Ze-Lin Tai Xu Zhang Hong-lian Li Yu Li Ke Wang |
| author_facet | Ling Qin Fan-kang Lin Yun-long Lv Ze-Lin Tai Xu Zhang Hong-lian Li Yu Li Ke Wang |
| author_sort | Ling Qin |
| collection | DOAJ |
| description | Abstract Sorghum (Sorghum bicolor) is an important food and feed crop. Root-lesion nematodes (Pratylenchus spp.) are a group of pathogenic nematodes that cause severe economic losses in various food and cash crops. This study identified diseased sorghum plants with stunted growth and brown, rotting roots in sorghum fields in Shanxi Province, China. A species of root-lesion nematode was isolated by modified Baermann funnel method and named the GL-1 population. Afterward, the GL-1 population of root-lesion nematodes was identified as P. coffeae through a combination of morphological, rDNA-ITS and rDNA-28 S D2-D3 region techniques for molecular biological identification. We also conducted greenhouse experiments to assess the parasitism and pathogenicity of GL-1 and four other P. coffeae populations on sorghum through pot inoculation. At 60 days after inoculation, the results indicated that all five populations of P. coffeae were capable of infecting and causing damage to the sorghum plants. Sorghum is a suitable host for P. coffeae (with a reproduction factor > 1). Moreover, compared with those in the control group, the aboveground fresh weights and root fresh weights of sorghum in the five inoculation groups were significantly lower, and brown spots or even necrotic rot appeared on the roots. All five populations were highly pathogenic to sorghum, but there were significant differences in pathogenicity among the populations. This study provides a scientific basis for identifying and detecting root-lesion nematodes in sorghum. |
| format | Article |
| id | doaj-art-da58b82204be4bf79f77e359471ec04f |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-da58b82204be4bf79f77e359471ec04f2025-01-26T12:17:59ZengBMCBMC Microbiology1471-21802025-01-0125111110.1186/s12866-025-03759-1Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in ChinaLing Qin0Fan-kang Lin1Yun-long Lv2Ze-Lin Tai3Xu Zhang4Hong-lian Li5Yu Li6Ke Wang7College of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityCollege of Plant Protection, Henan Agricultural UniversityAbstract Sorghum (Sorghum bicolor) is an important food and feed crop. Root-lesion nematodes (Pratylenchus spp.) are a group of pathogenic nematodes that cause severe economic losses in various food and cash crops. This study identified diseased sorghum plants with stunted growth and brown, rotting roots in sorghum fields in Shanxi Province, China. A species of root-lesion nematode was isolated by modified Baermann funnel method and named the GL-1 population. Afterward, the GL-1 population of root-lesion nematodes was identified as P. coffeae through a combination of morphological, rDNA-ITS and rDNA-28 S D2-D3 region techniques for molecular biological identification. We also conducted greenhouse experiments to assess the parasitism and pathogenicity of GL-1 and four other P. coffeae populations on sorghum through pot inoculation. At 60 days after inoculation, the results indicated that all five populations of P. coffeae were capable of infecting and causing damage to the sorghum plants. Sorghum is a suitable host for P. coffeae (with a reproduction factor > 1). Moreover, compared with those in the control group, the aboveground fresh weights and root fresh weights of sorghum in the five inoculation groups were significantly lower, and brown spots or even necrotic rot appeared on the roots. All five populations were highly pathogenic to sorghum, but there were significant differences in pathogenicity among the populations. This study provides a scientific basis for identifying and detecting root-lesion nematodes in sorghum.https://doi.org/10.1186/s12866-025-03759-1Sorghum bicolorRoot-lesion nematodeIdentificationParasitismPathogenicity |
| spellingShingle | Ling Qin Fan-kang Lin Yun-long Lv Ze-Lin Tai Xu Zhang Hong-lian Li Yu Li Ke Wang Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China BMC Microbiology Sorghum bicolor Root-lesion nematode Identification Parasitism Pathogenicity |
| title | Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China |
| title_full | Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China |
| title_fullStr | Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China |
| title_full_unstemmed | Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China |
| title_short | Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China |
| title_sort | identification of pratylenchus coffeae as a causal agent of root rot disease in sorghum bicolor in china |
| topic | Sorghum bicolor Root-lesion nematode Identification Parasitism Pathogenicity |
| url | https://doi.org/10.1186/s12866-025-03759-1 |
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