Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations
Roseburia intestinalis is one of the most abundant and important butyrate-producing human gut anaerobic bacteria that plays an important role in maintaining health and is a potential next-generation probiotic. We investigated the pangenome of 16 distinct strains, isolated over several decades, ident...
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| Format: | Article |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2025.2473516 |
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| author | Indrani Mukhopadhya Jennifer C. Martin Sophie Shaw Martin Gutierrez-Torrejon Nikoleta Boteva Aileen J. McKinley Silvia W. Gratz Karen P. Scott |
| author_facet | Indrani Mukhopadhya Jennifer C. Martin Sophie Shaw Martin Gutierrez-Torrejon Nikoleta Boteva Aileen J. McKinley Silvia W. Gratz Karen P. Scott |
| author_sort | Indrani Mukhopadhya |
| collection | DOAJ |
| description | Roseburia intestinalis is one of the most abundant and important butyrate-producing human gut anaerobic bacteria that plays an important role in maintaining health and is a potential next-generation probiotic. We investigated the pangenome of 16 distinct strains, isolated over several decades, identifying local and time-specific adaptations. More than 50% of the genes in each individual strain were assigned to the core genome, and 77% of the cloud genes were unique to individual strains, revealing the high level of genome conservation. Co-carriage of the same enzymes involved in carbohydrate binding and degradation in all strains highlighted major pathways in carbohydrate utilization and reveal the importance of xylan, starch and mannose as key growth substrates. A single strain had adapted to use rhamnose as a sole growth substrate, the first time this has been reported. The ubiquitous presence of motility and sporulation gene clusters demonstrates the importance of these phenotypes for gut survival and acquisition of this bacterium. More than half the strains contained functional, potentially transferable, tetracycline resistance genes. This study advances our understanding of the importance of R. intestinalis within the gut ecosystem by elucidating conserved metabolic characteristics among different strains, isolated from different locations. This information will help to devise dietary strategies to increase the abundance of this species providing health benefits. |
| format | Article |
| id | doaj-art-da4245c7bd4a402fa67e110ef1e3b18e |
| institution | DOAJ |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-da4245c7bd4a402fa67e110ef1e3b18e2025-08-20T03:12:47ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2473516Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locationsIndrani Mukhopadhya0Jennifer C. Martin1Sophie Shaw2Martin Gutierrez-Torrejon3Nikoleta Boteva4Aileen J. McKinley5Silvia W. Gratz6Karen P. Scott7Gut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKGut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKCentre for Genome Enabled Biology and Medicine, University of Aberdeen, Aberdeen, UKGut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKGut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKDepartment of Surgery, Aberdeen Royal Infirmary Foresterhill, Aberdeen, UKGut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKGut Microbiology Group, Rowett Institute, University of Aberdeen, Aberdeen, UKRoseburia intestinalis is one of the most abundant and important butyrate-producing human gut anaerobic bacteria that plays an important role in maintaining health and is a potential next-generation probiotic. We investigated the pangenome of 16 distinct strains, isolated over several decades, identifying local and time-specific adaptations. More than 50% of the genes in each individual strain were assigned to the core genome, and 77% of the cloud genes were unique to individual strains, revealing the high level of genome conservation. Co-carriage of the same enzymes involved in carbohydrate binding and degradation in all strains highlighted major pathways in carbohydrate utilization and reveal the importance of xylan, starch and mannose as key growth substrates. A single strain had adapted to use rhamnose as a sole growth substrate, the first time this has been reported. The ubiquitous presence of motility and sporulation gene clusters demonstrates the importance of these phenotypes for gut survival and acquisition of this bacterium. More than half the strains contained functional, potentially transferable, tetracycline resistance genes. This study advances our understanding of the importance of R. intestinalis within the gut ecosystem by elucidating conserved metabolic characteristics among different strains, isolated from different locations. This information will help to devise dietary strategies to increase the abundance of this species providing health benefits.https://www.tandfonline.com/doi/10.1080/19490976.2025.2473516Roseburia intestinalispangenomebutyratecarbohydrate utilizationCAZymeantimicrobial resistance |
| spellingShingle | Indrani Mukhopadhya Jennifer C. Martin Sophie Shaw Martin Gutierrez-Torrejon Nikoleta Boteva Aileen J. McKinley Silvia W. Gratz Karen P. Scott Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations Gut Microbes Roseburia intestinalis pangenome butyrate carbohydrate utilization CAZyme antimicrobial resistance |
| title | Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations |
| title_full | Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations |
| title_fullStr | Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations |
| title_full_unstemmed | Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations |
| title_short | Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in Roseburia intestinalis isolates across diverse geographical locations |
| title_sort | novel insights into carbohydrate utilisation antimicrobial resistance and sporulation potential in roseburia intestinalis isolates across diverse geographical locations |
| topic | Roseburia intestinalis pangenome butyrate carbohydrate utilization CAZyme antimicrobial resistance |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2025.2473516 |
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