Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?

The integration of forestry and agriculture has promoted edible fungi cultivation in forest understory spaces. However, the impact of spent mushroom substrates on forest soils remains unclear. This study explored the use of seafood mushroom spent substrates (SMS) and grass substrates to cultivate &l...

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Main Authors: Jing Li, Fengju Jiang, Xiaoyue Di, Qi Lai, Dongwei Feng, Yi Zeng, Yufang Lei, Yijia Yin, Biaosheng Lin, Xiuling He, Penghu Liu, Zhanxi Lin, Xiongjie Lin, Dongmei Lin
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Agriculture
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Online Access:https://www.mdpi.com/2077-0472/15/14/1533
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author Jing Li
Fengju Jiang
Xiaoyue Di
Qi Lai
Dongwei Feng
Yi Zeng
Yufang Lei
Yijia Yin
Biaosheng Lin
Xiuling He
Penghu Liu
Zhanxi Lin
Xiongjie Lin
Dongmei Lin
author_facet Jing Li
Fengju Jiang
Xiaoyue Di
Qi Lai
Dongwei Feng
Yi Zeng
Yufang Lei
Yijia Yin
Biaosheng Lin
Xiuling He
Penghu Liu
Zhanxi Lin
Xiongjie Lin
Dongmei Lin
author_sort Jing Li
collection DOAJ
description The integration of forestry and agriculture has promoted edible fungi cultivation in forest understory spaces. However, the impact of spent mushroom substrates on forest soils remains unclear. This study explored the use of seafood mushroom spent substrates (SMS) and grass substrates to cultivate <i>Dictyophora indusiata</i>. After cultivation, soil pH stabilized, organic carbon increased by 34.02–62.24%, total nitrogen rose 1.1–1.9-fold, while soil catalase activity increased by 43.78–100.41% and laccase activity surged 3.3–11.2-fold. The 49% <i>Cenchrus fungigraminus</i> and 49% SMS treatment yielded the highest 4-coumaric acid levels in the soil, while all treatments reduced maslinic and pantothenic acid content. SMS as padding material with <i>C. fungigraminus</i> enhanced soil bacterial diversity in the first and following years. Environmental factors and organic acids influenced the recruitment of genus of Latescibacterota, <i>Acidothermus</i>, <i>Rokubacteriales</i>, <i>Candidatus solibacter</i>, and <i>Bacillus</i>, altering organic acid composition. In conclusion, cultivating <i>D. indusiata</i> understory enhanced environmental characteristics, microbial dynamics, and organic acid profiles in forests’ soil in short time.
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publishDate 2025-07-01
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spelling doaj-art-da3e59d9d46d4356a8aaa32d0a19da852025-08-20T03:36:15ZengMDPI AGAgriculture2077-04722025-07-011514153310.3390/agriculture15141533Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?Jing Li0Fengju Jiang1Xiaoyue Di2Qi Lai3Dongwei Feng4Yi Zeng5Yufang Lei6Yijia Yin7Biaosheng Lin8Xiuling He9Penghu Liu10Zhanxi Lin11Xiongjie Lin12Dongmei Lin13National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Science, Longyan University, Longyan 364012, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaNational Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe integration of forestry and agriculture has promoted edible fungi cultivation in forest understory spaces. However, the impact of spent mushroom substrates on forest soils remains unclear. This study explored the use of seafood mushroom spent substrates (SMS) and grass substrates to cultivate <i>Dictyophora indusiata</i>. After cultivation, soil pH stabilized, organic carbon increased by 34.02–62.24%, total nitrogen rose 1.1–1.9-fold, while soil catalase activity increased by 43.78–100.41% and laccase activity surged 3.3–11.2-fold. The 49% <i>Cenchrus fungigraminus</i> and 49% SMS treatment yielded the highest 4-coumaric acid levels in the soil, while all treatments reduced maslinic and pantothenic acid content. SMS as padding material with <i>C. fungigraminus</i> enhanced soil bacterial diversity in the first and following years. Environmental factors and organic acids influenced the recruitment of genus of Latescibacterota, <i>Acidothermus</i>, <i>Rokubacteriales</i>, <i>Candidatus solibacter</i>, and <i>Bacillus</i>, altering organic acid composition. In conclusion, cultivating <i>D. indusiata</i> understory enhanced environmental characteristics, microbial dynamics, and organic acid profiles in forests’ soil in short time.https://www.mdpi.com/2077-0472/15/14/1533bamboo mushroom<i>Cenchrus fungigraminus</i>soil organic acidsspent mushroom substratesunderstory environment
spellingShingle Jing Li
Fengju Jiang
Xiaoyue Di
Qi Lai
Dongwei Feng
Yi Zeng
Yufang Lei
Yijia Yin
Biaosheng Lin
Xiuling He
Penghu Liu
Zhanxi Lin
Xiongjie Lin
Dongmei Lin
Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
Agriculture
bamboo mushroom
<i>Cenchrus fungigraminus</i>
soil organic acids
spent mushroom substrates
understory environment
title Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
title_full Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
title_fullStr Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
title_full_unstemmed Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
title_short Is the Cultivation of <i>Dictyophora indusiata</i> with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
title_sort is the cultivation of i dictyophora indusiata i with grass based substrates an efficacious and sustainable approach for enhancing the understory soil environment
topic bamboo mushroom
<i>Cenchrus fungigraminus</i>
soil organic acids
spent mushroom substrates
understory environment
url https://www.mdpi.com/2077-0472/15/14/1533
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