Cytotoxicity of substances leached from a conventional and a self-etching adhesive system on human pulp fibroblasts
The use of adhesives for direct dental pulp capping is not advisable, due to its harmful effects to the tissue. However, new adhesive systems are often released, and self-etching systems seem to be less toxic than conventional ones. The purpose of this study was to compare the in vitro cytotoxicity...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual Paulista
2011-09-01
|
| Series: | Brazilian Dental Science |
| Subjects: | |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/699 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The use of adhesives for direct dental pulp capping is not advisable, due to its harmful effects to the tissue. However, new adhesive systems are often released, and self-etching systems seem to be less toxic than conventional ones. The purpose of this study was to compare the in vitro cytotoxicity of substances leached from calcium hydroxide and two adhesive systems on human dental pulp fibroblasts. Cell culture media conditioned by Calcium Hydroxide (CH), Single Bond (SB), Clearfill Protect Bond primer (CP) or Clearfill Protect Bond resin (CB) were applied to human pulp fibroblasts. Fresh cell culture medium was used in the Control group. The number of viable cells was obtained through the MTT reduction assay. Data were compared by ANOVA and Tukey's test (p≤0.05). The mean number of viable cells was 3.9x103(±0.75) for the control group, which was similar to those found in the CH group (4.31x103±0.87). Statistical differences were found among the groups (p<0.001),with the cell viability decreasing significantly with SB (0.09x103±0.06) and CP (0.28x103±0.08) when compared to CH and control groups. CB (2.37x103±0.72) was significantly less cytotoxic than CP and SB, but more cytotoxic than CH. It was concluded that Single Bond and Clearfill Protect Bond primer release substances that decrease cell viability of human dental pulp cells in culture. The use of bonding systems for direct pulp capping is not recommended, since they are cytotoxic. |
|---|---|
| ISSN: | 2178-6011 |