An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery.
Meeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production. Host-induced gene silencing has shown great potential for controlling pest and diseases in crop plants. However, while delivery...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-10-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005901&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850078208781713408 |
|---|---|
| author | Aline Koch Dagmar Biedenkopf Alexandra Furch Lennart Weber Oliver Rossbach Eltayb Abdellatef Lukas Linicus Jan Johannsmeier Lukas Jelonek Alexander Goesmann Vinitha Cardoza John McMillan Tobias Mentzel Karl-Heinz Kogel |
| author_facet | Aline Koch Dagmar Biedenkopf Alexandra Furch Lennart Weber Oliver Rossbach Eltayb Abdellatef Lukas Linicus Jan Johannsmeier Lukas Jelonek Alexander Goesmann Vinitha Cardoza John McMillan Tobias Mentzel Karl-Heinz Kogel |
| author_sort | Aline Koch |
| collection | DOAJ |
| description | Meeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production. Host-induced gene silencing has shown great potential for controlling pest and diseases in crop plants. However, while delivery of inhibitory noncoding double-stranded (ds)RNA by transgenic expression is a promising concept, it requires the generation of transgenic crop plants which may cause substantial delay for application strategies depending on the transformability and genetic stability of the crop plant species. Using the agronomically important barley-Fusarium graminearum pathosystem, we alternatively demonstrate that a spray application of a long noncoding dsRNA (791 nt CYP3-dsRNA), which targets the three fungal cytochrome P450 lanosterol C-14α-demethylases, required for biosynthesis of fungal ergosterol, inhibits fungal growth in the directly sprayed (local) as well as the non-sprayed (distal) parts of detached leaves. Unexpectedly, efficient spray-induced control of fungal infections in the distal tissue involved passage of CYP3-dsRNA via the plant vascular system and processing into small interfering (si)RNAs by fungal DICER-LIKE 1 (FgDCL-1) after uptake by the pathogen. We discuss important consequences of this new finding on future RNA-based disease control strategies. Given the ease of design, high specificity, and applicability to diverse pathogens, the use of target-specific dsRNA as an anti-fungal agent offers unprecedented potential as a new plant protection strategy. |
| format | Article |
| id | doaj-art-da390cb4c83c4c00ae1df4303612934e |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-da390cb4c83c4c00ae1df4303612934e2025-08-20T02:45:37ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-10-011210e100590110.1371/journal.ppat.1005901An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery.Aline KochDagmar BiedenkopfAlexandra FurchLennart WeberOliver RossbachEltayb AbdellatefLukas LinicusJan JohannsmeierLukas JelonekAlexander GoesmannVinitha CardozaJohn McMillanTobias MentzelKarl-Heinz KogelMeeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production. Host-induced gene silencing has shown great potential for controlling pest and diseases in crop plants. However, while delivery of inhibitory noncoding double-stranded (ds)RNA by transgenic expression is a promising concept, it requires the generation of transgenic crop plants which may cause substantial delay for application strategies depending on the transformability and genetic stability of the crop plant species. Using the agronomically important barley-Fusarium graminearum pathosystem, we alternatively demonstrate that a spray application of a long noncoding dsRNA (791 nt CYP3-dsRNA), which targets the three fungal cytochrome P450 lanosterol C-14α-demethylases, required for biosynthesis of fungal ergosterol, inhibits fungal growth in the directly sprayed (local) as well as the non-sprayed (distal) parts of detached leaves. Unexpectedly, efficient spray-induced control of fungal infections in the distal tissue involved passage of CYP3-dsRNA via the plant vascular system and processing into small interfering (si)RNAs by fungal DICER-LIKE 1 (FgDCL-1) after uptake by the pathogen. We discuss important consequences of this new finding on future RNA-based disease control strategies. Given the ease of design, high specificity, and applicability to diverse pathogens, the use of target-specific dsRNA as an anti-fungal agent offers unprecedented potential as a new plant protection strategy.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005901&type=printable |
| spellingShingle | Aline Koch Dagmar Biedenkopf Alexandra Furch Lennart Weber Oliver Rossbach Eltayb Abdellatef Lukas Linicus Jan Johannsmeier Lukas Jelonek Alexander Goesmann Vinitha Cardoza John McMillan Tobias Mentzel Karl-Heinz Kogel An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. PLoS Pathogens |
| title | An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. |
| title_full | An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. |
| title_fullStr | An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. |
| title_full_unstemmed | An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. |
| title_short | An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery. |
| title_sort | rnai based control of fusarium graminearum infections through spraying of long dsrnas involves a plant passage and is controlled by the fungal silencing machinery |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005901&type=printable |
| work_keys_str_mv | AT alinekoch anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT dagmarbiedenkopf anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT alexandrafurch anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lennartweber anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT oliverrossbach anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT eltaybabdellatef anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lukaslinicus anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT janjohannsmeier anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lukasjelonek anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT alexandergoesmann anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT vinithacardoza anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT johnmcmillan anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT tobiasmentzel anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT karlheinzkogel anrnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT alinekoch rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT dagmarbiedenkopf rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT alexandrafurch rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lennartweber rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT oliverrossbach rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT eltaybabdellatef rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lukaslinicus rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT janjohannsmeier rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT lukasjelonek rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT alexandergoesmann rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT vinithacardoza rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT johnmcmillan rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT tobiasmentzel rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery AT karlheinzkogel rnaibasedcontroloffusariumgraminearuminfectionsthroughsprayingoflongdsrnasinvolvesaplantpassageandiscontrolledbythefungalsilencingmachinery |