Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation
Enucleation-induced adrenal regeneration is a highly controlled process; however, only some elements involved in this process have been recognized. Therefore, we performed studies on regenerating rat adrenals. Microarray RNA analysis and QPCR revealed that enucleation resulted in a rapid elevation o...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/130359 |
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| author | Marianna Tyczewska Marcin Rucinski Agnieszka Ziolkowska Marta Szyszka Marcin Trejter Anna Hochol-Molenda Krzysztof W. Nowak Ludwik K. Malendowicz |
| author_facet | Marianna Tyczewska Marcin Rucinski Agnieszka Ziolkowska Marta Szyszka Marcin Trejter Anna Hochol-Molenda Krzysztof W. Nowak Ludwik K. Malendowicz |
| author_sort | Marianna Tyczewska |
| collection | DOAJ |
| description | Enucleation-induced adrenal regeneration is a highly controlled process; however, only some elements involved in this process have been recognized. Therefore, we performed studies on regenerating rat adrenals. Microarray RNA analysis and QPCR revealed that enucleation resulted in a rapid elevation of expression of genes involved in response to wounding, defense response, and in immunological processes. Factors encoded by these genes obscure possible priming effects of various cytokines on initiation of regeneration. In regenerating adrenals we identified over 100 up- or downregulated genes involved in adrenocortical cell proliferation. The changes were most significant at days 2-3 after enucleation and their number decreased during regeneration. For example, expression analysis revealed a notable upregulation of the growth arrest gene, Gadd45, only 24 hours after surgery while expression of cyclin B1 and Cdk1 genes was notably elevated between days 1–8 of regeneration. These changes were accompanied by changes in expression levels of numerous growth factors and immediate-early transcription factors genes. Despite notable differences in mechanisms of adrenal and liver regeneration, in regenerating adrenals we identified genes, the expression of which is well recognized in regenerating liver. Thus, it seems legitimate to suggest that, in the rat, the general model of liver and adrenal regeneration demonstrate some degree of similarity. |
| format | Article |
| id | doaj-art-da3662038bce4d7baddf3234306d1b21 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-da3662038bce4d7baddf3234306d1b212025-08-20T03:24:25ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/130359130359Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell ProliferationMarianna Tyczewska0Marcin Rucinski1Agnieszka Ziolkowska2Marta Szyszka3Marcin Trejter4Anna Hochol-Molenda5Krzysztof W. Nowak6Ludwik K. Malendowicz7Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandEnucleation-induced adrenal regeneration is a highly controlled process; however, only some elements involved in this process have been recognized. Therefore, we performed studies on regenerating rat adrenals. Microarray RNA analysis and QPCR revealed that enucleation resulted in a rapid elevation of expression of genes involved in response to wounding, defense response, and in immunological processes. Factors encoded by these genes obscure possible priming effects of various cytokines on initiation of regeneration. In regenerating adrenals we identified over 100 up- or downregulated genes involved in adrenocortical cell proliferation. The changes were most significant at days 2-3 after enucleation and their number decreased during regeneration. For example, expression analysis revealed a notable upregulation of the growth arrest gene, Gadd45, only 24 hours after surgery while expression of cyclin B1 and Cdk1 genes was notably elevated between days 1–8 of regeneration. These changes were accompanied by changes in expression levels of numerous growth factors and immediate-early transcription factors genes. Despite notable differences in mechanisms of adrenal and liver regeneration, in regenerating adrenals we identified genes, the expression of which is well recognized in regenerating liver. Thus, it seems legitimate to suggest that, in the rat, the general model of liver and adrenal regeneration demonstrate some degree of similarity.http://dx.doi.org/10.1155/2014/130359 |
| spellingShingle | Marianna Tyczewska Marcin Rucinski Agnieszka Ziolkowska Marta Szyszka Marcin Trejter Anna Hochol-Molenda Krzysztof W. Nowak Ludwik K. Malendowicz Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation International Journal of Endocrinology |
| title | Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation |
| title_full | Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation |
| title_fullStr | Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation |
| title_full_unstemmed | Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation |
| title_short | Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation |
| title_sort | enucleation induced rat adrenal gland regeneration expression profile of selected genes involved in control of adrenocortical cell proliferation |
| url | http://dx.doi.org/10.1155/2014/130359 |
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