Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS
Goat milk has gained significant attention due to its unique nutritional properties. In this study, we conducted a comprehensive lipidomics analysis of goat milk fed by a normal diet (C), dietary supplement with Schizochytrium sp. (S), flax seed (F), and their mixture (M) using UPLC Q-TOF-MS/MS. A t...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525004286 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850254099228917760 |
|---|---|
| author | Jie Wang Shiqian Ran Xin Lv Dan Wang Hong Chen Fang Wei |
| author_facet | Jie Wang Shiqian Ran Xin Lv Dan Wang Hong Chen Fang Wei |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | Goat milk has gained significant attention due to its unique nutritional properties. In this study, we conducted a comprehensive lipidomics analysis of goat milk fed by a normal diet (C), dietary supplement with Schizochytrium sp. (S), flax seed (F), and their mixture (M) using UPLC Q-TOF-MS/MS. A total of 638 lipid molecules covering 16 subclasses were identified, with TG constituting over 97 % of the total lipids. Dietary supplementation significantly reduced glycerolipids (GL) content while markedly increasing ω-3 polyunsaturated fatty acid (PUFA) levels, with group M showing the most pronounced effects on enhancing ω-3 PUFA content. The percentages of DHA-TG and DHA-GPL increased by more than 10-fold and 4-fold, respectively. Flax seed specifically increased ALA containing lipids, whereas Schizochytrium sp. predominantly enriched DHA and DPA containing lipids. Multivariate statistical analysis revealed distinct lipid profiles among the groups, highlighting the significant impact of dietary supplementation on lipid composition. In conclusion, this study provides a robust theoretical foundation for the production, nutritional evaluation, identification, and application of ω-3 PUFA-enriched goat milk. |
| format | Article |
| id | doaj-art-da33cab76fd64faeb04e9b49f5e35c5e |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-da33cab76fd64faeb04e9b49f5e35c5e2025-08-20T01:57:12ZengElsevierFood Chemistry: X2590-15752025-05-012810258110.1016/j.fochx.2025.102581Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MSJie Wang0Shiqian Ran1Xin Lv2Dan Wang3Hong Chen4Fang Wei5Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR ChinaKey Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China; Zunyi Rural Development Service Center, Zunyi, Guizhou 563000, PR ChinaKey Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR ChinaKey Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR ChinaKey Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China; Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR ChinaKey Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, PR China; Corresponding author at: Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China.Goat milk has gained significant attention due to its unique nutritional properties. In this study, we conducted a comprehensive lipidomics analysis of goat milk fed by a normal diet (C), dietary supplement with Schizochytrium sp. (S), flax seed (F), and their mixture (M) using UPLC Q-TOF-MS/MS. A total of 638 lipid molecules covering 16 subclasses were identified, with TG constituting over 97 % of the total lipids. Dietary supplementation significantly reduced glycerolipids (GL) content while markedly increasing ω-3 polyunsaturated fatty acid (PUFA) levels, with group M showing the most pronounced effects on enhancing ω-3 PUFA content. The percentages of DHA-TG and DHA-GPL increased by more than 10-fold and 4-fold, respectively. Flax seed specifically increased ALA containing lipids, whereas Schizochytrium sp. predominantly enriched DHA and DPA containing lipids. Multivariate statistical analysis revealed distinct lipid profiles among the groups, highlighting the significant impact of dietary supplementation on lipid composition. In conclusion, this study provides a robust theoretical foundation for the production, nutritional evaluation, identification, and application of ω-3 PUFA-enriched goat milk.http://www.sciencedirect.com/science/article/pii/S2590157525004286LipidomicsGoat milkDietary supplementedΩ-3 polyunsaturated fatty acid |
| spellingShingle | Jie Wang Shiqian Ran Xin Lv Dan Wang Hong Chen Fang Wei Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS Food Chemistry: X Lipidomics Goat milk Dietary supplemented Ω-3 polyunsaturated fatty acid |
| title | Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS |
| title_full | Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS |
| title_fullStr | Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS |
| title_full_unstemmed | Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS |
| title_short | Comprehensive lipidomics analysis reveals the effects in goat milk from different ω-3 PUFA dietary supplementation by UPLC Q-TOF-MS/MS |
| title_sort | comprehensive lipidomics analysis reveals the effects in goat milk from different ω 3 pufa dietary supplementation by uplc q tof ms ms |
| topic | Lipidomics Goat milk Dietary supplemented Ω-3 polyunsaturated fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525004286 |
| work_keys_str_mv | AT jiewang comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms AT shiqianran comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms AT xinlv comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms AT danwang comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms AT hongchen comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms AT fangwei comprehensivelipidomicsanalysisrevealstheeffectsingoatmilkfromdifferentō3pufadietarysupplementationbyuplcqtofmsms |