Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers
This study evaluated the antioxidant activity of ethanol extracts of Osmanthus fragrans (Thunb.) Lour. flowers (EOF) and identified phenolic compounds in EOF using liquid chromatography-mass spectrometry. Nine compounds, 3-O-caffeoylquinic acid, caffeic acid 4-O-glucoside, salidroside, 5-O-coumaroyl...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2017-12-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2017.67.issue-4/pjfns-2017-0008/pjfns-2017-0008.xml?format=INT |
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| author | Li Hong-Ling Chai Zhi Shen Garry X. Li Chun-Yang |
| author_facet | Li Hong-Ling Chai Zhi Shen Garry X. Li Chun-Yang |
| author_sort | Li Hong-Ling |
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| description | This study evaluated the antioxidant activity of ethanol extracts of Osmanthus fragrans (Thunb.) Lour. flowers (EOF) and identified phenolic compounds in EOF using liquid chromatography-mass spectrometry. Nine compounds, 3-O-caffeoylquinic acid, caffeic acid 4-O-glucoside, salidroside, 5-O-coumaroylquinic acid, 4-O-coumaroylquinic acid, acteoside, ligustroside, fucosterol and arjunolic acid were identified. To our knowledge, caffeic acid 4-O-glucoside, 5-O-coumaroylquinic acid and 4-O-coumaroylquinic acid have not been detected in EOF. In vitro antioxidant activity analysis demonstrated that EOF possessed strong DPPH and ABTS radicals scavenging activity with EC50 values at 0.26±0.06 mg/mL and 0.36±0.01 mg/mL, respectively, and reducing power with Ab0.5 value at 13.04±0.16 μg/mL. The value of oxygen radical absorbance capacity (ORAC) was 333.23±13.39 μmol Trolox/g. Antioxidant activity assay in human umbilical vein endothelial cells (HUVEC) showed that the activity of superoxide dismutase (SOD) was significantly improved and the reactive oxygen species (ROS) was removed effectively from cells when treated with EOF of 300~3000 μg/L. |
| format | Article |
| id | doaj-art-da3077fb5b50409d858c4ce1b0652218 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-da3077fb5b50409d858c4ce1b06522182025-02-02T13:44:01ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072017-12-0167431732610.1515/pjfns-2017-0008pjfns-2017-0008Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. FlowersLi Hong-Ling0Chai Zhi1Shen Garry X.2Li Chun-Yang3College of Food Science and Technology, Nanjing Agricultural University, Nanjing, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, ChinaDepartments of Internal Medicine and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, R3E 3P4, CanadaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, ChinaThis study evaluated the antioxidant activity of ethanol extracts of Osmanthus fragrans (Thunb.) Lour. flowers (EOF) and identified phenolic compounds in EOF using liquid chromatography-mass spectrometry. Nine compounds, 3-O-caffeoylquinic acid, caffeic acid 4-O-glucoside, salidroside, 5-O-coumaroylquinic acid, 4-O-coumaroylquinic acid, acteoside, ligustroside, fucosterol and arjunolic acid were identified. To our knowledge, caffeic acid 4-O-glucoside, 5-O-coumaroylquinic acid and 4-O-coumaroylquinic acid have not been detected in EOF. In vitro antioxidant activity analysis demonstrated that EOF possessed strong DPPH and ABTS radicals scavenging activity with EC50 values at 0.26±0.06 mg/mL and 0.36±0.01 mg/mL, respectively, and reducing power with Ab0.5 value at 13.04±0.16 μg/mL. The value of oxygen radical absorbance capacity (ORAC) was 333.23±13.39 μmol Trolox/g. Antioxidant activity assay in human umbilical vein endothelial cells (HUVEC) showed that the activity of superoxide dismutase (SOD) was significantly improved and the reactive oxygen species (ROS) was removed effectively from cells when treated with EOF of 300~3000 μg/L.http://www.degruyter.com/view/j/pjfns.2017.67.issue-4/pjfns-2017-0008/pjfns-2017-0008.xml?format=INTethanol extracts of Osmanthus fragrans flowersantioxidantpolyphenol profilescultured endothelial cells |
| spellingShingle | Li Hong-Ling Chai Zhi Shen Garry X. Li Chun-Yang Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers Polish Journal of Food and Nutrition Sciences ethanol extracts of Osmanthus fragrans flowers antioxidant polyphenol profiles cultured endothelial cells |
| title | Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers |
| title_full | Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers |
| title_fullStr | Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers |
| title_full_unstemmed | Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers |
| title_short | Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers |
| title_sort | polyphenol profiles and antioxidant properties of ethanol extracts from osmanthus fragrans thunb lour flowers |
| topic | ethanol extracts of Osmanthus fragrans flowers antioxidant polyphenol profiles cultured endothelial cells |
| url | http://www.degruyter.com/view/j/pjfns.2017.67.issue-4/pjfns-2017-0008/pjfns-2017-0008.xml?format=INT |
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