A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit
The cell cycle is the process by which a cell duplicates its DNA during S-phase and divides its chromosomes during M-phase, creating two genetically identical daughter cells. Cell cycle events are ordered by synthesis and degradation of key cell regulators and by phosphorylation and dephosphorylatio...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shared Science Publishers OG
2018-07-01
|
| Series: | Microbial Cell |
| Subjects: | |
| Online Access: | http://microbialcell.com/researcharticles/a-global-view-of-substrate-phosphorylation-and-dephosphorylation-during-budding-yeast-mitotic-exit/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850050850852962304 |
|---|---|
| author | Sandra A. Touati Frank Uhlmann |
| author_facet | Sandra A. Touati Frank Uhlmann |
| author_sort | Sandra A. Touati |
| collection | DOAJ |
| description | The cell cycle is the process by which a cell duplicates its DNA during S-phase and divides its chromosomes during M-phase, creating two genetically identical daughter cells. Cell cycle events are ordered by synthesis and degradation of key cell regulators and by phosphorylation and dephosphorylation of numerous substrates. Phosphorylation can alter the activity, interactions or subcellular localization of a protein. A substrate’s phosphorylation status is the readout of competing activities of kinases and phosphatases that target each of its phosphorylation sites. In our recent study (EMBO J. 37, e98745), we performed time-resolved global phosphoproteome analysis of a period during the cell cycle known as mitotic exit. During this time, numerous cell biological events happen in fast succession but in strict order. First, at the metaphase to anaphase transition, the mitotic spindle elongates to pull maximally condensed chromosomes to opposite cell halves. Shortly after that, spindles disassemble and chromosomes decondense, before finally cell division is completed by cytokinesis. Our time-resolved phosphoproteome analysis of this period in budding yeast provided a survey of the principles of phosphoregulation used to order these events. |
| format | Article |
| id | doaj-art-da2fd750992349c583321fa2ac5bd8a9 |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2018-07-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-da2fd750992349c583321fa2ac5bd8a92025-08-20T02:53:20ZengShared Science Publishers OGMicrobial Cell2311-26382018-07-015838939210.15698/mic2018.08.644A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exitSandra A. Touati0Frank Uhlmann1Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.The cell cycle is the process by which a cell duplicates its DNA during S-phase and divides its chromosomes during M-phase, creating two genetically identical daughter cells. Cell cycle events are ordered by synthesis and degradation of key cell regulators and by phosphorylation and dephosphorylation of numerous substrates. Phosphorylation can alter the activity, interactions or subcellular localization of a protein. A substrate’s phosphorylation status is the readout of competing activities of kinases and phosphatases that target each of its phosphorylation sites. In our recent study (EMBO J. 37, e98745), we performed time-resolved global phosphoproteome analysis of a period during the cell cycle known as mitotic exit. During this time, numerous cell biological events happen in fast succession but in strict order. First, at the metaphase to anaphase transition, the mitotic spindle elongates to pull maximally condensed chromosomes to opposite cell halves. Shortly after that, spindles disassemble and chromosomes decondense, before finally cell division is completed by cytokinesis. Our time-resolved phosphoproteome analysis of this period in budding yeast provided a survey of the principles of phosphoregulation used to order these events.http://microbialcell.com/researcharticles/a-global-view-of-substrate-phosphorylation-and-dephosphorylation-during-budding-yeast-mitotic-exit/Cell cyclekinasesmitosismitotic exitphosphatasesphosphoproteomics |
| spellingShingle | Sandra A. Touati Frank Uhlmann A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit Microbial Cell Cell cycle kinases mitosis mitotic exit phosphatases phosphoproteomics |
| title | A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| title_full | A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| title_fullStr | A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| title_full_unstemmed | A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| title_short | A global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| title_sort | global view of substrate phosphorylation and dephosphorylation during budding yeast mitotic exit |
| topic | Cell cycle kinases mitosis mitotic exit phosphatases phosphoproteomics |
| url | http://microbialcell.com/researcharticles/a-global-view-of-substrate-phosphorylation-and-dephosphorylation-during-budding-yeast-mitotic-exit/ |
| work_keys_str_mv | AT sandraatouati aglobalviewofsubstratephosphorylationanddephosphorylationduringbuddingyeastmitoticexit AT frankuhlmann aglobalviewofsubstratephosphorylationanddephosphorylationduringbuddingyeastmitoticexit AT sandraatouati globalviewofsubstratephosphorylationanddephosphorylationduringbuddingyeastmitoticexit AT frankuhlmann globalviewofsubstratephosphorylationanddephosphorylationduringbuddingyeastmitoticexit |