The potential value of microRNA-409-5p-mediated negative regulation of USP7 in the diagnosis and treatment of acute myocardial infarction
Abstract Purpose This study aimed to explore the effects of ubiquitin-specific peptidase 7 (USP7) on acute myocardial infarction (AMI) and the negative regulation of USP7 by microRNA-409-5p (miR-409-5p). Methods Clinical data were collected from patients admitted to the Cardiology Department of Yan’...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-025-04590-2 |
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| Summary: | Abstract Purpose This study aimed to explore the effects of ubiquitin-specific peptidase 7 (USP7) on acute myocardial infarction (AMI) and the negative regulation of USP7 by microRNA-409-5p (miR-409-5p). Methods Clinical data were collected from patients admitted to the Cardiology Department of Yan’an Hospital of Kunming City between July 2020 and July 2021. The participants included patients with AMI (AMI; n = 30), stable angina pectoris (SAP; n = 30), and chest pain syndrome (CPS; n = 30) and healthy controls (n = 30). The expression levels of miR-409-5p and USP7 were analysed using Quantitative real-time polymerase chain reaction (qRT‒PCR) and Western blotting (WB). Finally, a dual-luciferase assay was performed to verify the interaction between miR-409-5p and USP7. Results The expression level of miR-409-5p was significantly lower (all p < 0.05), whereas the expression level of USP7 was elevated in patients with AMI compared with those in the other three groups (all p < 0.05). A dual-luciferase assay demonstrated that miR-409-5p binds to USP7 3’UTP to inhibit luciferase expression. Compared with cells transfected with mutation fragments and a luciferase reporter vector with microRNA-409-5p mimics and USP7 3'UTR binding and mutation sites, the luminescence level of cells with miR-409-5p was approximately 40% lower. Additionally, miRNA-409-5p was inversely correlated with cTnI (p = 0.004). Conclusion USP7 plays a significant role in AMI via negative regulation by miR-409-5p. Both miR-409-5p and USP7 hold key potential as early diagnostic biomarkers and therapeutic targets for AMI in the future. |
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| ISSN: | 1471-2261 |