Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood
Human schistosomiasis is a chronic neglected tropical disease caused by blood flukes of the genus Schistosoma, infecting 250 million people worldwide, mostly in sub-Saharan Africa. Recently, thousands of cases have been reported in immigrants to non-endemic countries, including Italy. Serological sc...
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Elsevier
2025-03-01
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| Series: | Travel Medicine and Infectious Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1477893925000134 |
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| author | Margherita Ortalli Bianca Granozzi Michele Bacchiega Concettina Di Lillo Greta Roncarati Silvia Stefania Longoni Cristina Mazzi Elisa Vanino Zeno Bisoffi Stefania Varani |
| author_facet | Margherita Ortalli Bianca Granozzi Michele Bacchiega Concettina Di Lillo Greta Roncarati Silvia Stefania Longoni Cristina Mazzi Elisa Vanino Zeno Bisoffi Stefania Varani |
| author_sort | Margherita Ortalli |
| collection | DOAJ |
| description | Human schistosomiasis is a chronic neglected tropical disease caused by blood flukes of the genus Schistosoma, infecting 250 million people worldwide, mostly in sub-Saharan Africa. Recently, thousands of cases have been reported in immigrants to non-endemic countries, including Italy. Serological screening is recommended but so far, no accurate point-of-care (POC) and lab-free test is available.We carried out a prospective evaluation of the accuracy of a new immunochromatographic test (Black- ICT, IgG-IgM) using finger prick blood for screening of schistosomiasis at the University Hospital of Bologna. Eligible immigrants were recruited regardless the presence of symptoms. The other tests used were microscopy on stools and urine, a serum-ICT (SCHISTOSOMA ICT IgG-IgM, LDBIO Diagnostics), an ELISA (NovaLisa Schistosoma mansoni IgG, Novatec) and a Western Blot (SCHISTO II Western Blot IgG, LDBIO Diagnostics). Statistical analysis was performed using a Bayesian latent class model.We enrolled 198 subjects in the study. Black-ICT had a sensitivity of 86.6 % (95 % credible interval 76.9–94.7) and a specificity of 88.4 % (82.0–94.3). At the estimated prevalence level for the study sample, 32.6 % (25.5–40.0), the positive and negative predictive values were 78.2 % (66.4–89.4) and 93.2 % (87.7–97.6), respectively. Good agreement was found with the other antibody tests, with the highest sensitivity being observed for serum-ICT (91.0 %, 84.7–96.4) and the highest specificity for ELISA (92.6 %, 87.5–96.7).The novel POC test for schistosomiasis showed satisfactory results and could improve the detection of this parasitic infection in non-endemic settings, as the lab-free approach could greatly expand the target group. |
| format | Article |
| id | doaj-art-da294eef021d4bbd9cbe2589df3d17ae |
| institution | Kabale University |
| issn | 1873-0442 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Travel Medicine and Infectious Disease |
| spelling | doaj-art-da294eef021d4bbd9cbe2589df3d17ae2025-01-31T05:10:57ZengElsevierTravel Medicine and Infectious Disease1873-04422025-03-0164102807Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick bloodMargherita Ortalli0Bianca Granozzi1Michele Bacchiega2Concettina Di Lillo3Greta Roncarati4Silvia Stefania Longoni5Cristina Mazzi6Elisa Vanino7Zeno Bisoffi8Stefania Varani9Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, Bologna, Italy; Microbiology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyInfectious Diseases Unit, Department for Integrated Infectious Risk Management, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyDepartment of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, Bologna, ItalyMicrobiology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyMicrobiology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, ItalyIRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, Verona, Italy; Corresponding author.IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, Verona, ItalyInfectious Diseases Unit, Ospedale ''Santa Maria delle Croci'', AUSL Romagna, Ravenna, ItalyIRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, Verona, ItalyDepartment of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, Bologna, ItalyHuman schistosomiasis is a chronic neglected tropical disease caused by blood flukes of the genus Schistosoma, infecting 250 million people worldwide, mostly in sub-Saharan Africa. Recently, thousands of cases have been reported in immigrants to non-endemic countries, including Italy. Serological screening is recommended but so far, no accurate point-of-care (POC) and lab-free test is available.We carried out a prospective evaluation of the accuracy of a new immunochromatographic test (Black- ICT, IgG-IgM) using finger prick blood for screening of schistosomiasis at the University Hospital of Bologna. Eligible immigrants were recruited regardless the presence of symptoms. The other tests used were microscopy on stools and urine, a serum-ICT (SCHISTOSOMA ICT IgG-IgM, LDBIO Diagnostics), an ELISA (NovaLisa Schistosoma mansoni IgG, Novatec) and a Western Blot (SCHISTO II Western Blot IgG, LDBIO Diagnostics). Statistical analysis was performed using a Bayesian latent class model.We enrolled 198 subjects in the study. Black-ICT had a sensitivity of 86.6 % (95 % credible interval 76.9–94.7) and a specificity of 88.4 % (82.0–94.3). At the estimated prevalence level for the study sample, 32.6 % (25.5–40.0), the positive and negative predictive values were 78.2 % (66.4–89.4) and 93.2 % (87.7–97.6), respectively. Good agreement was found with the other antibody tests, with the highest sensitivity being observed for serum-ICT (91.0 %, 84.7–96.4) and the highest specificity for ELISA (92.6 %, 87.5–96.7).The novel POC test for schistosomiasis showed satisfactory results and could improve the detection of this parasitic infection in non-endemic settings, as the lab-free approach could greatly expand the target group.http://www.sciencedirect.com/science/article/pii/S1477893925000134SchistosomiasisImmunochromatographic testFinger-prick bloodScreening |
| spellingShingle | Margherita Ortalli Bianca Granozzi Michele Bacchiega Concettina Di Lillo Greta Roncarati Silvia Stefania Longoni Cristina Mazzi Elisa Vanino Zeno Bisoffi Stefania Varani Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood Travel Medicine and Infectious Disease Schistosomiasis Immunochromatographic test Finger-prick blood Screening |
| title | Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood |
| title_full | Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood |
| title_fullStr | Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood |
| title_full_unstemmed | Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood |
| title_short | Screening for schistosomiasis in a non-endemic setting: Accuracy of a rapid antibody test using finger prick blood |
| title_sort | screening for schistosomiasis in a non endemic setting accuracy of a rapid antibody test using finger prick blood |
| topic | Schistosomiasis Immunochromatographic test Finger-prick blood Screening |
| url | http://www.sciencedirect.com/science/article/pii/S1477893925000134 |
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