Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em>
Hyaluronic acid (HA) is a well-known functional marine polysaccharide. The utilization and derivative development of HA are of great interest. Hyaluronan lyase has wide application prospects in the production of HA oligosaccharides and lower molecular weight HA. In this study, a strain of <i>E...
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2024-08-01
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| author | Linjing Zhang Jiayu Jiang Wei Liu Lianlong Wang Zhiyuan Yao Heng Li Jinsong Gong Chuanli Kang Lei Liu Zhenghong Xu Jinsong Shi |
| author_facet | Linjing Zhang Jiayu Jiang Wei Liu Lianlong Wang Zhiyuan Yao Heng Li Jinsong Gong Chuanli Kang Lei Liu Zhenghong Xu Jinsong Shi |
| author_sort | Linjing Zhang |
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| description | Hyaluronic acid (HA) is a well-known functional marine polysaccharide. The utilization and derivative development of HA are of great interest. Hyaluronan lyase has wide application prospects in the production of HA oligosaccharides and lower molecular weight HA. In this study, a strain of <i>Enterobacter asburiae</i> CGJ001 with high hyaluronan lyase activity was screened from industrial wastewater. This strain exhibited an impressive enzyme activity of 40,576 U/mL after being incubated for 14 h. Whole genome sequencing analysis revealed that <i>E. asburiae</i> CGJ001 contained a cluster of genes involved in HA degradation, transport, and metabolism. A newly identified enzyme responsible for glycosaminoglycan degradation was designated as HylEP0006. A strain of <i>E. coli</i> BL21(DE3)/<i>p</i>ET-22b(+)<i>-hylEP0006</i> was successfully constructed. HylEP0006 exhibited optimal degradation at 40 °C and pH 7.0, showing a high activity of 950,168.3 U/mg. HylEP0006 showed specific activity against HA. The minimum degradation fragment of HylEP0006 was hyaluronan tetrasaccharides, and HylEP0006 could efficiently degrade HA into unsaturated disaccharides (HA2), with HA2 as the final product. These characteristics indicate that HylEP0006 has a potential application prospect for the extraction and utilization of hyaluronic acid. |
| format | Article |
| id | doaj-art-da205c2b1c8145f7bbfde60da10ef050 |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj-art-da205c2b1c8145f7bbfde60da10ef0502025-08-20T01:55:38ZengMDPI AGMarine Drugs1660-33972024-08-0122939910.3390/md22090399Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em>Linjing Zhang0Jiayu Jiang1Wei Liu2Lianlong Wang3Zhiyuan Yao4Heng Li5Jinsong Gong6Chuanli Kang7Lei Liu8Zhenghong Xu9Jinsong Shi10Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaShandong Engineering Laboratory of Sodium Hyaluronate and Its Derivatives, Shandong Focusfreda Biotech Co., Ltd., Qufu 273165, ChinaShandong Engineering Laboratory of Sodium Hyaluronate and Its Derivatives, Shandong Focusfreda Biotech Co., Ltd., Qufu 273165, ChinaInnovation Center for Advanced Brewing Science and Technology, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaHyaluronic acid (HA) is a well-known functional marine polysaccharide. The utilization and derivative development of HA are of great interest. Hyaluronan lyase has wide application prospects in the production of HA oligosaccharides and lower molecular weight HA. In this study, a strain of <i>Enterobacter asburiae</i> CGJ001 with high hyaluronan lyase activity was screened from industrial wastewater. This strain exhibited an impressive enzyme activity of 40,576 U/mL after being incubated for 14 h. Whole genome sequencing analysis revealed that <i>E. asburiae</i> CGJ001 contained a cluster of genes involved in HA degradation, transport, and metabolism. A newly identified enzyme responsible for glycosaminoglycan degradation was designated as HylEP0006. A strain of <i>E. coli</i> BL21(DE3)/<i>p</i>ET-22b(+)<i>-hylEP0006</i> was successfully constructed. HylEP0006 exhibited optimal degradation at 40 °C and pH 7.0, showing a high activity of 950,168.3 U/mg. HylEP0006 showed specific activity against HA. The minimum degradation fragment of HylEP0006 was hyaluronan tetrasaccharides, and HylEP0006 could efficiently degrade HA into unsaturated disaccharides (HA2), with HA2 as the final product. These characteristics indicate that HylEP0006 has a potential application prospect for the extraction and utilization of hyaluronic acid.https://www.mdpi.com/1660-3397/22/9/399hyaluronan lyasehyaluronic acidhyaluronic acid oligosaccharides<i>Enterobacter asburiae</i> |
| spellingShingle | Linjing Zhang Jiayu Jiang Wei Liu Lianlong Wang Zhiyuan Yao Heng Li Jinsong Gong Chuanli Kang Lei Liu Zhenghong Xu Jinsong Shi Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> Marine Drugs hyaluronan lyase hyaluronic acid hyaluronic acid oligosaccharides <i>Enterobacter asburiae</i> |
| title | Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> |
| title_full | Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> |
| title_fullStr | Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> |
| title_full_unstemmed | Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> |
| title_short | Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em> |
| title_sort | identification and characterization of a highly active hyaluronan lyase from em enterobacter asburiae em |
| topic | hyaluronan lyase hyaluronic acid hyaluronic acid oligosaccharides <i>Enterobacter asburiae</i> |
| url | https://www.mdpi.com/1660-3397/22/9/399 |
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