Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats
Objective. Blood glucose concentrations of type 1 diabetic rats are vulnerable, especially to stress and trauma. The present study aimed to investigate the fasting endogenous glucose production and skeletal muscle glucose uptake of Streptozotocin induced type 1 diabetic rats using an unstressed vein...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/743798 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849472882314313728 |
|---|---|
| author | Shichun Du Hui Wu Xiao Xu Ying Meng Fangzhen Xia Hualing Zhai Yingli Lu |
| author_facet | Shichun Du Hui Wu Xiao Xu Ying Meng Fangzhen Xia Hualing Zhai Yingli Lu |
| author_sort | Shichun Du |
| collection | DOAJ |
| description | Objective. Blood glucose concentrations of type 1 diabetic rats are vulnerable, especially to stress and trauma. The present study aimed to investigate the fasting endogenous glucose production and skeletal muscle glucose uptake of Streptozotocin induced type 1 diabetic rats using an unstressed vein and artery implantation of catheters at the tails of the rats as a platform. Research Design and Methods. Streptozotocin (65 mg·kg−1) was administered to induce type 1 diabetic state. The unstressed approach of catheters of vein and artery at the tails of the rats was established before the isotope tracer injection. Dynamic measurement of fasting endogenous glucose production was assessed by continuously infusing stable isotope [6, 6-2H2] glucose, while skeletal muscle glucose uptake by bolus injecting radioactively labeled [1-14C]-2-deoxy-glucose. Results. Streptozotocin induced type 1 diabetic rats displayed polydipsia, polyphagia, and polyuria along with overt hyperglycemia and hypoinsulinemia. They also had enhanced fasting endogenous glucose production and reduced glucose uptake in skeletal muscle compared to nondiabetic rats. Conclusions. The dual catheters implantation at the tails of the rats together with isotope tracers injection is a save time, unstressed, and feasible approach to explore the glucose metabolism in animal models in vivo. |
| format | Article |
| id | doaj-art-da1bcae63fd64c02992931b266a878ce |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-da1bcae63fd64c02992931b266a878ce2025-08-20T03:24:22ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/743798743798Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic RatsShichun Du0Hui Wu1Xiao Xu2Ying Meng3Fangzhen Xia4Hualing Zhai5Yingli Lu6Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Endocrinology, Zhejiang Province People’s Hospital, Hangzhou 310014, ChinaInstitute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaInstitute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaInstitute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaInstitute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaInstitute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaObjective. Blood glucose concentrations of type 1 diabetic rats are vulnerable, especially to stress and trauma. The present study aimed to investigate the fasting endogenous glucose production and skeletal muscle glucose uptake of Streptozotocin induced type 1 diabetic rats using an unstressed vein and artery implantation of catheters at the tails of the rats as a platform. Research Design and Methods. Streptozotocin (65 mg·kg−1) was administered to induce type 1 diabetic state. The unstressed approach of catheters of vein and artery at the tails of the rats was established before the isotope tracer injection. Dynamic measurement of fasting endogenous glucose production was assessed by continuously infusing stable isotope [6, 6-2H2] glucose, while skeletal muscle glucose uptake by bolus injecting radioactively labeled [1-14C]-2-deoxy-glucose. Results. Streptozotocin induced type 1 diabetic rats displayed polydipsia, polyphagia, and polyuria along with overt hyperglycemia and hypoinsulinemia. They also had enhanced fasting endogenous glucose production and reduced glucose uptake in skeletal muscle compared to nondiabetic rats. Conclusions. The dual catheters implantation at the tails of the rats together with isotope tracers injection is a save time, unstressed, and feasible approach to explore the glucose metabolism in animal models in vivo.http://dx.doi.org/10.1155/2014/743798 |
| spellingShingle | Shichun Du Hui Wu Xiao Xu Ying Meng Fangzhen Xia Hualing Zhai Yingli Lu Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats Journal of Diabetes Research |
| title | Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats |
| title_full | Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats |
| title_fullStr | Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats |
| title_full_unstemmed | Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats |
| title_short | Tracing Fasting Glucose Fluxes with Unstressed Catheter Approach in Streptozotocin Induced Diabetic Rats |
| title_sort | tracing fasting glucose fluxes with unstressed catheter approach in streptozotocin induced diabetic rats |
| url | http://dx.doi.org/10.1155/2014/743798 |
| work_keys_str_mv | AT shichundu tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT huiwu tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT xiaoxu tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT yingmeng tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT fangzhenxia tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT hualingzhai tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats AT yinglilu tracingfastingglucosefluxeswithunstressedcatheterapproachinstreptozotocininduceddiabeticrats |