The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice
Mengwei Song,1,2,* Mi Han,1,2,* Haoyu Zhang,1 Yifan Yang,1,2 Yange Tian,1,2 Jiansheng Li,2,3 Jie Zhao1,2 1Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, People’s Republic of China; 2Henan Key Laboratory of Chinese Medicine for Respirator...
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Dove Medical Press
2025-01-01
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| author | Song M Han M Zhang H Yang Y Tian Y Li J Zhao J |
| author_facet | Song M Han M Zhang H Yang Y Tian Y Li J Zhao J |
| author_sort | Song M |
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| description | Mengwei Song,1,2,* Mi Han,1,2,* Haoyu Zhang,1 Yifan Yang,1,2 Yange Tian,1,2 Jiansheng Li,2,3 Jie Zhao1,2 1Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, People’s Republic of China; 2Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed by Henan Province and Education Ministry of P.R. China, Henan University of Chinese Medicine, Zhengzhou, 450046, People’s Republic of China; 3Department of Respiratory Diseases, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jie Zhao, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengdong, Zhengzhou, Henan, 450046, People’s Republic of China, Tel +86-15100165967, Email Jiezhao1702@163.comPurpose: The effective compound combination of Bufei Yishen formula III (ECC-BYF III) has shown protective effects against chronic obstructive pulmonary disease (COPD). However, its effect on mitochondrial dysfunction remains unclear. The current study aimed to investigate the effect of ECC-BYF III on mitochondrial dysfunction in COPD mice and elucidate its potential mechanisms.Methods: Twenty-eight BALB/c mice were randomized into four groups: control, model, ECC-BYF III, and NAC (N-acetylcysteine) groups. A COPD model was established using cigarette smoke and Klebsiella pneumoniae for 8 weeks. The mice in the ECC-BYF III group were treated with ECC-BYF III (7.7 mg/kg/d), and the NAC group was treated with NAC (78 mg/kg/d) for eight weeks. Mice in the control and model groups were administered with 0.5% sodium carboxymethyl cellulose (25 mL/kg/d) for eight weeks. Then pulmonary function, histopathology, inflammatory factor levels, mitochondrial ultrastructure and function, and immunoblotting analyses were evaluated.Results: Compared with the model, ECC-BYF III significantly improved the decline in pulmonary function and histopathological changes. Furthermore, ECC-BYF III ameliorated mitochondrial dysfunction by restoring the mitochondrial membrane potential, increasing mitochondrial complex I activity, and decreasing tumor necrosis factor-α (TNF-α) level and protein expressions of SH3BP5 (Sab), Phospho-JNK (P-JNK), and cleaved CASP3.Conclusion: The results suggest that the potential therapeutic benefit of ECC-BYF III against mitochondrial dysfunction in COPD is due to the inhibition of the JNK/Sab pathway, which will help to further understand the potential mechanisms of ECC-BYF III in the treatment of COPD. Keywords: chronic obstructive pulmonary disease, Chinese medicine, mitochondrion, apoptosis, JNK/Sab pathway |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-da15f904b1914c298e2827cf2dd229972025-01-27T18:05:33ZengDove Medical PressDrug Design, Development and Therapy1177-88812025-01-01Volume 1952553899562The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD MiceSong MHan MZhang HYang YTian YLi JZhao JMengwei Song,1,2,* Mi Han,1,2,* Haoyu Zhang,1 Yifan Yang,1,2 Yange Tian,1,2 Jiansheng Li,2,3 Jie Zhao1,2 1Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, People’s Republic of China; 2Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed by Henan Province and Education Ministry of P.R. China, Henan University of Chinese Medicine, Zhengzhou, 450046, People’s Republic of China; 3Department of Respiratory Diseases, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jie Zhao, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengdong, Zhengzhou, Henan, 450046, People’s Republic of China, Tel +86-15100165967, Email Jiezhao1702@163.comPurpose: The effective compound combination of Bufei Yishen formula III (ECC-BYF III) has shown protective effects against chronic obstructive pulmonary disease (COPD). However, its effect on mitochondrial dysfunction remains unclear. The current study aimed to investigate the effect of ECC-BYF III on mitochondrial dysfunction in COPD mice and elucidate its potential mechanisms.Methods: Twenty-eight BALB/c mice were randomized into four groups: control, model, ECC-BYF III, and NAC (N-acetylcysteine) groups. A COPD model was established using cigarette smoke and Klebsiella pneumoniae for 8 weeks. The mice in the ECC-BYF III group were treated with ECC-BYF III (7.7 mg/kg/d), and the NAC group was treated with NAC (78 mg/kg/d) for eight weeks. Mice in the control and model groups were administered with 0.5% sodium carboxymethyl cellulose (25 mL/kg/d) for eight weeks. Then pulmonary function, histopathology, inflammatory factor levels, mitochondrial ultrastructure and function, and immunoblotting analyses were evaluated.Results: Compared with the model, ECC-BYF III significantly improved the decline in pulmonary function and histopathological changes. Furthermore, ECC-BYF III ameliorated mitochondrial dysfunction by restoring the mitochondrial membrane potential, increasing mitochondrial complex I activity, and decreasing tumor necrosis factor-α (TNF-α) level and protein expressions of SH3BP5 (Sab), Phospho-JNK (P-JNK), and cleaved CASP3.Conclusion: The results suggest that the potential therapeutic benefit of ECC-BYF III against mitochondrial dysfunction in COPD is due to the inhibition of the JNK/Sab pathway, which will help to further understand the potential mechanisms of ECC-BYF III in the treatment of COPD. Keywords: chronic obstructive pulmonary disease, Chinese medicine, mitochondrion, apoptosis, JNK/Sab pathwayhttps://www.dovepress.com/the-effective-compound-combination-of-bufei-yishen-formula-iii-improve-peer-reviewed-fulltext-article-DDDTchronic obstructive pulmonary diseasechinese medicinemitochondrionapoptosisjnk/sab pathway. |
| spellingShingle | Song M Han M Zhang H Yang Y Tian Y Li J Zhao J The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice Drug Design, Development and Therapy chronic obstructive pulmonary disease chinese medicine mitochondrion apoptosis jnk/sab pathway. |
| title | The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice |
| title_full | The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice |
| title_fullStr | The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice |
| title_full_unstemmed | The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice |
| title_short | The Effective Compound Combination of Bufei Yishen Formula III Improves the Mitochondrial Dysfunction via Inhibiting JNK/Sab Pathway in COPD Mice |
| title_sort | effective compound combination of bufei yishen formula iii improves the mitochondrial dysfunction via inhibiting jnk sab pathway in copd mice |
| topic | chronic obstructive pulmonary disease chinese medicine mitochondrion apoptosis jnk/sab pathway. |
| url | https://www.dovepress.com/the-effective-compound-combination-of-bufei-yishen-formula-iii-improve-peer-reviewed-fulltext-article-DDDT |
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