Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots
Abstract Plant roots perceive heat stress (HS) and adapt their architecture accordingly, which in turn influence the yield in crops. Investigating their heterogeneity and cell type-specific response to HS is essential for improving crop resilience. Here, we generate single-cell transcriptional lands...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55485-3 |
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| author | Ting Wang Fanhua Wang Shuhan Deng Kailai Wang Dan Feng Fan Xu Weijun Guo Jia Yu Yue Wu Hada Wuriyanghan Shang-Tong Li Xiaofeng Gu Liang Le Li Pu |
| author_facet | Ting Wang Fanhua Wang Shuhan Deng Kailai Wang Dan Feng Fan Xu Weijun Guo Jia Yu Yue Wu Hada Wuriyanghan Shang-Tong Li Xiaofeng Gu Liang Le Li Pu |
| author_sort | Ting Wang |
| collection | DOAJ |
| description | Abstract Plant roots perceive heat stress (HS) and adapt their architecture accordingly, which in turn influence the yield in crops. Investigating their heterogeneity and cell type-specific response to HS is essential for improving crop resilience. Here, we generate single-cell transcriptional landscape of maize (Zea mays) roots in response to HS. We characterize 15 cell clusters corresponding to 9 major cell types and identify cortex as the main root cell type responsive to HS with the most differentially expressed genes and its trajectory being preferentially affected upon HS. We find that cortex size strongly correlated with heat tolerance that is experimentally validated by using inbred lines and genetic mutation analysis of one candidate gene in maize, providing potential HS tolerance indicator and targets for crop improvement. Moreover, interspecies comparison reveals conserved root cell types and core markers in response to HS in plants, which are experimentally validated. These results provide a universal atlas for unraveling the transcriptional programs that specify and maintain the cell identity of maize roots in response to HS at a cell type-specific level. |
| format | Article |
| id | doaj-art-da13d6689b1f4bf3a6ff680d91328896 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-da13d6689b1f4bf3a6ff680d913288962025-01-05T12:39:08ZengNature PortfolioNature Communications2041-17232025-01-0116111710.1038/s41467-024-55485-3Single-cell transcriptomes reveal spatiotemporal heat stress response in maize rootsTing Wang0Fanhua Wang1Shuhan Deng2Kailai Wang3Dan Feng4Fan Xu5Weijun Guo6Jia Yu7Yue Wu8Hada Wuriyanghan9Shang-Tong Li10Xiaofeng Gu11Liang Le12Li Pu13Biotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesGlbizzia Biosciences Co., LtdGlbizzia Biosciences Co., LtdBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesSchool of Life Science, Inner Mongolia UniversityGlbizzia Biosciences Co., LtdBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesAbstract Plant roots perceive heat stress (HS) and adapt their architecture accordingly, which in turn influence the yield in crops. Investigating their heterogeneity and cell type-specific response to HS is essential for improving crop resilience. Here, we generate single-cell transcriptional landscape of maize (Zea mays) roots in response to HS. We characterize 15 cell clusters corresponding to 9 major cell types and identify cortex as the main root cell type responsive to HS with the most differentially expressed genes and its trajectory being preferentially affected upon HS. We find that cortex size strongly correlated with heat tolerance that is experimentally validated by using inbred lines and genetic mutation analysis of one candidate gene in maize, providing potential HS tolerance indicator and targets for crop improvement. Moreover, interspecies comparison reveals conserved root cell types and core markers in response to HS in plants, which are experimentally validated. These results provide a universal atlas for unraveling the transcriptional programs that specify and maintain the cell identity of maize roots in response to HS at a cell type-specific level.https://doi.org/10.1038/s41467-024-55485-3 |
| spellingShingle | Ting Wang Fanhua Wang Shuhan Deng Kailai Wang Dan Feng Fan Xu Weijun Guo Jia Yu Yue Wu Hada Wuriyanghan Shang-Tong Li Xiaofeng Gu Liang Le Li Pu Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots Nature Communications |
| title | Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| title_full | Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| title_fullStr | Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| title_full_unstemmed | Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| title_short | Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| title_sort | single cell transcriptomes reveal spatiotemporal heat stress response in maize roots |
| url | https://doi.org/10.1038/s41467-024-55485-3 |
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