Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens
Avian pathogenic Escherichia coli (APEC) infections result in significant economic losses and reduced animal welfare. Historically, antibiotics and vaccinations currently control APEC infections in poultry, however, antibiotic-resistant strains and heterologous serotypes limit their effectiveness. M...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912401215X |
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| author | Zhuoran Li Xueying Wang Dianzhong Zheng Fuzhen Han Yue Li Han Zhou Jiaxuan Li Wen Cui Yanping Jiang Xiaona Wang Weichun Xie Lijie Tang |
| author_facet | Zhuoran Li Xueying Wang Dianzhong Zheng Fuzhen Han Yue Li Han Zhou Jiaxuan Li Wen Cui Yanping Jiang Xiaona Wang Weichun Xie Lijie Tang |
| author_sort | Zhuoran Li |
| collection | DOAJ |
| description | Avian pathogenic Escherichia coli (APEC) infections result in significant economic losses and reduced animal welfare. Historically, antibiotics and vaccinations currently control APEC infections in poultry, however, antibiotic-resistant strains and heterologous serotypes limit their effectiveness. Meanwhile, antibiotic-resistant strains can be transmitted to humans via contact with animals, food or their environment. Probiotics and antimicrobial peptides (AMPs) are potential alternatives to antibiotics and represent promising strategies to combat APEC. Bovine lactoferricin and lactoferrampin possess anti-bacterial, anti-inflammatory, and anti-oxidant properties. Lactococcus lactis (L. lactis) is an excellent vector for delivering recombinant proteins. In this research, we generated a recombinant L. lactis strain MG1363 expressing lactoferrin peptides, which was labeled with a fluorescent marker mCherry and lacked an antibiotic resistance gene (LL-EFLmC). Our investigation focused on the impact of LL-EFLmC strain on the gut microbiota composition and avian pathogenic E. coli O78 challenge. Our findings indicate that LL-EFLmC exhibits inhibitory effects against APEC-O78 and Staphylococcus aureus CVCC26003 (S. aureus CVCC26003) in vitro. Furthermore, the inclusion of LL-EFLmC in chicken feed significantly improved the average daily intake and gain to feed ratio. Additionally, LL-EFLmC treatment resulted in a significant increase in serum IgG and intestinal mucus SIgA levels. Administration of LL-EFLmC was found to effectively suppress APEC-O78 infection and mitigate the expression of pro-inflammatory cytokines, including IL-1β, IL-12, IFN-γ, and TNF-α. Additionally, 16S rDNA sequencing data revealed that LL-EFLmC was able to restore the intestinal flora that had been disrupted by APEC-O78. These findings suggest that LL-EFLmC may serve as a promising feed additive and antibiotic alternative in chicken production, due to its potential to enhance immune regulation, promote growth, and confer resistance against APEC-O78 infection. |
| format | Article |
| id | doaj-art-da0845e2fff44310951ab12f7041fa39 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-da0845e2fff44310951ab12f7041fa392025-01-22T05:40:47ZengElsevierPoultry Science0032-57912025-01-011041104637Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickensZhuoran Li0Xueying Wang1Dianzhong Zheng2Fuzhen Han3Yue Li4Han Zhou5Jiaxuan Li6Wen Cui7Yanping Jiang8Xiaona Wang9Weichun Xie10Lijie Tang11College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCorresponding author.; College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin 150030, ChinaAvian pathogenic Escherichia coli (APEC) infections result in significant economic losses and reduced animal welfare. Historically, antibiotics and vaccinations currently control APEC infections in poultry, however, antibiotic-resistant strains and heterologous serotypes limit their effectiveness. Meanwhile, antibiotic-resistant strains can be transmitted to humans via contact with animals, food or their environment. Probiotics and antimicrobial peptides (AMPs) are potential alternatives to antibiotics and represent promising strategies to combat APEC. Bovine lactoferricin and lactoferrampin possess anti-bacterial, anti-inflammatory, and anti-oxidant properties. Lactococcus lactis (L. lactis) is an excellent vector for delivering recombinant proteins. In this research, we generated a recombinant L. lactis strain MG1363 expressing lactoferrin peptides, which was labeled with a fluorescent marker mCherry and lacked an antibiotic resistance gene (LL-EFLmC). Our investigation focused on the impact of LL-EFLmC strain on the gut microbiota composition and avian pathogenic E. coli O78 challenge. Our findings indicate that LL-EFLmC exhibits inhibitory effects against APEC-O78 and Staphylococcus aureus CVCC26003 (S. aureus CVCC26003) in vitro. Furthermore, the inclusion of LL-EFLmC in chicken feed significantly improved the average daily intake and gain to feed ratio. Additionally, LL-EFLmC treatment resulted in a significant increase in serum IgG and intestinal mucus SIgA levels. Administration of LL-EFLmC was found to effectively suppress APEC-O78 infection and mitigate the expression of pro-inflammatory cytokines, including IL-1β, IL-12, IFN-γ, and TNF-α. Additionally, 16S rDNA sequencing data revealed that LL-EFLmC was able to restore the intestinal flora that had been disrupted by APEC-O78. These findings suggest that LL-EFLmC may serve as a promising feed additive and antibiotic alternative in chicken production, due to its potential to enhance immune regulation, promote growth, and confer resistance against APEC-O78 infection.http://www.sciencedirect.com/science/article/pii/S003257912401215XAvian pathogenic Escherichia coliLactoferricin-lactoferrampinLactococcus lactisChickenAntibiotic |
| spellingShingle | Zhuoran Li Xueying Wang Dianzhong Zheng Fuzhen Han Yue Li Han Zhou Jiaxuan Li Wen Cui Yanping Jiang Xiaona Wang Weichun Xie Lijie Tang Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens Poultry Science Avian pathogenic Escherichia coli Lactoferricin-lactoferrampin Lactococcus lactis Chicken Antibiotic |
| title | Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens |
| title_full | Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens |
| title_fullStr | Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens |
| title_full_unstemmed | Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens |
| title_short | Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens |
| title_sort | oral delivery lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic escherichia coli in chickens |
| topic | Avian pathogenic Escherichia coli Lactoferricin-lactoferrampin Lactococcus lactis Chicken Antibiotic |
| url | http://www.sciencedirect.com/science/article/pii/S003257912401215X |
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