Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles
Microplastic (MP) represent a pervasive and escalating threat to aquatic ecosystems, impacting organisms from cellular to population levels. To investigate the immediate molecular impacts of MP exposure, we exposed Daphnia magna, a keystone species in freshwater ecosystems, to polystyrene microplast...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016579 |
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| author | Young Sang Kwon Chang-Beom Park Seung-Min Lee Jin-Woo Park Yeong-Jin Kim Jong-Hwan Kim Jong-Su Seo |
| author_facet | Young Sang Kwon Chang-Beom Park Seung-Min Lee Jin-Woo Park Yeong-Jin Kim Jong-Hwan Kim Jong-Su Seo |
| author_sort | Young Sang Kwon |
| collection | DOAJ |
| description | Microplastic (MP) represent a pervasive and escalating threat to aquatic ecosystems, impacting organisms from cellular to population levels. To investigate the immediate molecular impacts of MP exposure, we exposed Daphnia magna, a keystone species in freshwater ecosystems, to polystyrene microplastic particles (5 μm, 5 μg/L) for 48 h. Through proteomic and biochemical analyses, we identified extensive disruptions in key physiological pathways. Notably, proteins involved in energy metabolism, including glycolysis and the tricarboxylic acid (TCA) cycle, were downregulated, suggesting a metabolic shift away from growth-related processes. Elevated levels of oxidative stress markers such as superoxide dismutase, catalase, and glutathione reductase reflected a pronounced response to reactive oxygen species. The upregulation of endocytosis-related proteins, including caveolin-1 (CAV1) and phosphatidylinositol-4-phosphate 5-kinase (PIP5K), highlights their role in actively internalizing and compartmentalizing MP, potentially as a protective mechanism against oxidative damage. These findings reveal that short-term MP exposure triggers a complex, multi-pathway stress response in D. magna, underscoring potential vulnerabilities that could impact broader ecological dynamics. This study emphasizes the urgency of understanding MP toxicity to guide environmental policies and conservation efforts aimed at mitigating the effects of plastic pollution. |
| format | Article |
| id | doaj-art-da05a826e5144475ac471b70a27ae146 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-da05a826e5144475ac471b70a27ae1462025-02-12T05:29:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117581Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particlesYoung Sang Kwon0Chang-Beom Park1Seung-Min Lee2Jin-Woo Park3Yeong-Jin Kim4Jong-Hwan Kim5Jong-Su Seo6Center for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Ecotoxicology and Environmental Future Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of KoreaCenter for Environmental Safety Research, Division of Gyeongnam Bio-Environmental Research, Korea Institute of Toxicology, Jinju 52834, Republic of Korea; Corresponding author.Microplastic (MP) represent a pervasive and escalating threat to aquatic ecosystems, impacting organisms from cellular to population levels. To investigate the immediate molecular impacts of MP exposure, we exposed Daphnia magna, a keystone species in freshwater ecosystems, to polystyrene microplastic particles (5 μm, 5 μg/L) for 48 h. Through proteomic and biochemical analyses, we identified extensive disruptions in key physiological pathways. Notably, proteins involved in energy metabolism, including glycolysis and the tricarboxylic acid (TCA) cycle, were downregulated, suggesting a metabolic shift away from growth-related processes. Elevated levels of oxidative stress markers such as superoxide dismutase, catalase, and glutathione reductase reflected a pronounced response to reactive oxygen species. The upregulation of endocytosis-related proteins, including caveolin-1 (CAV1) and phosphatidylinositol-4-phosphate 5-kinase (PIP5K), highlights their role in actively internalizing and compartmentalizing MP, potentially as a protective mechanism against oxidative damage. These findings reveal that short-term MP exposure triggers a complex, multi-pathway stress response in D. magna, underscoring potential vulnerabilities that could impact broader ecological dynamics. This study emphasizes the urgency of understanding MP toxicity to guide environmental policies and conservation efforts aimed at mitigating the effects of plastic pollution.http://www.sciencedirect.com/science/article/pii/S0147651324016579MicroplasticsDaphnia magnaProteomic analysisOxidative stressPlastic pollution impactBiomarker analysis |
| spellingShingle | Young Sang Kwon Chang-Beom Park Seung-Min Lee Jin-Woo Park Yeong-Jin Kim Jong-Hwan Kim Jong-Su Seo Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles Ecotoxicology and Environmental Safety Microplastics Daphnia magna Proteomic analysis Oxidative stress Plastic pollution impact Biomarker analysis |
| title | Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles |
| title_full | Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles |
| title_fullStr | Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles |
| title_full_unstemmed | Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles |
| title_short | Comprehensive analysis of proteomic and biochemical responses of Daphnia magna to short-term exposure to polystyrene microplastic particles |
| title_sort | comprehensive analysis of proteomic and biochemical responses of daphnia magna to short term exposure to polystyrene microplastic particles |
| topic | Microplastics Daphnia magna Proteomic analysis Oxidative stress Plastic pollution impact Biomarker analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016579 |
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