System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance
Purpose Bio-effects following thermal treatments are a function of the achieved temperature profile in tissue, which can be estimated across tumor volumes with real-time MRI thermometry (MRIT). Here, we report on expansion of a previously developed small-animal microwave hyperthermia system integrat...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | International Journal of Hyperthermia |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2022.2061727 |
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| author | Jan Sebek Tej B. Shrestha Matthew T. Basel Faraz Chamani Nooshin Zeinali Ivina Mali Macy Payne Sarah A. Timmerman Pegah Faridi Marla Pyle Martin O’Halloran M. Conall Dennedy Stefan H. Bossmann Punit Prakash |
| author_facet | Jan Sebek Tej B. Shrestha Matthew T. Basel Faraz Chamani Nooshin Zeinali Ivina Mali Macy Payne Sarah A. Timmerman Pegah Faridi Marla Pyle Martin O’Halloran M. Conall Dennedy Stefan H. Bossmann Punit Prakash |
| author_sort | Jan Sebek |
| collection | DOAJ |
| description | Purpose Bio-effects following thermal treatments are a function of the achieved temperature profile in tissue, which can be estimated across tumor volumes with real-time MRI thermometry (MRIT). Here, we report on expansion of a previously developed small-animal microwave hyperthermia system integrated with MRIT for delivering thermal ablation to subcutaneously implanted tumors in mice.Methods Computational models were employed to assess suitability of the 2.45 GHz microwave applicators for delivering ablation to subcutaneous tumor targets in mice. Phantoms and ex-vivo tissues were heated to temperatures in the range 47–67 °C with custom-made microwave applicators for validating MRIT with the proton resonance frequency shift method against fiberoptic thermometry. HAC15 tumors implanted in nude mice (n = 6) were ablated in vivo and monitored with MRIT in multiple planes. One day post ablation, animals were euthanized, and excised tumors were processed for viability assessment.Results Average absolute error between temperatures from fiberoptic sensors and MRIT was 0.6 °C across all ex-vivo ablations. During in-vivo experiments, tumors with volumes ranging between 5.4–35.9 mm3 (mean 14.2 mm3) were ablated (duration: 103–150 s) to achieve 55 °C at the tumor boundary. Thermal doses ≥240 CEM43 were achieved across 90.7–98.0% of tumor volumes for four cases. Ablations were incomplete for remaining cases, attributed to motion-affected thermometry. Thermal dose-based ablative tumor coverage agreed with viability assessment of excised tumors.Conclusions We have developed a system for delivering microwave ablation to subcutaneous tumors in small animals under MRIT guidance and demonstrated its performance in-vivo. |
| format | Article |
| id | doaj-art-d9fdac0c710b4e0fa41a7685cd71dcfb |
| institution | DOAJ |
| issn | 0265-6736 1464-5157 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj-art-d9fdac0c710b4e0fa41a7685cd71dcfb2025-08-20T03:13:25ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572022-12-0139158459410.1080/02656736.2022.2061727System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidanceJan Sebek0Tej B. Shrestha1Matthew T. Basel2Faraz Chamani3Nooshin Zeinali4Ivina Mali5Macy Payne6Sarah A. Timmerman7Pegah Faridi8Marla Pyle9Martin O’Halloran10M. Conall Dennedy11Stefan H. Bossmann12Punit Prakash13Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USADepartment of Anatomy and Physiology, Kansas State University, Manhattan, KS, USADepartment of Anatomy and Physiology, Kansas State University, Manhattan, KS, USADepartment of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USADepartment of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USADepartment of Chemistry, Kansas State University, Manhattan, KS, USADepartment of Chemistry, Kansas State University, Manhattan, KS, USADepartment of Anatomy and Physiology, Kansas State University, Manhattan, KS, USADepartment of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USADepartment of Anatomy and Physiology, Kansas State University, Manhattan, KS, USACollege of Medicine, Nursing and Health Sciences, National University of Ireland Galway, Galway, Republic of IrelandCollege of Medicine, Nursing and Health Sciences, National University of Ireland Galway, Galway, Republic of IrelandDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, USADepartment of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USAPurpose Bio-effects following thermal treatments are a function of the achieved temperature profile in tissue, which can be estimated across tumor volumes with real-time MRI thermometry (MRIT). Here, we report on expansion of a previously developed small-animal microwave hyperthermia system integrated with MRIT for delivering thermal ablation to subcutaneously implanted tumors in mice.Methods Computational models were employed to assess suitability of the 2.45 GHz microwave applicators for delivering ablation to subcutaneous tumor targets in mice. Phantoms and ex-vivo tissues were heated to temperatures in the range 47–67 °C with custom-made microwave applicators for validating MRIT with the proton resonance frequency shift method against fiberoptic thermometry. HAC15 tumors implanted in nude mice (n = 6) were ablated in vivo and monitored with MRIT in multiple planes. One day post ablation, animals were euthanized, and excised tumors were processed for viability assessment.Results Average absolute error between temperatures from fiberoptic sensors and MRIT was 0.6 °C across all ex-vivo ablations. During in-vivo experiments, tumors with volumes ranging between 5.4–35.9 mm3 (mean 14.2 mm3) were ablated (duration: 103–150 s) to achieve 55 °C at the tumor boundary. Thermal doses ≥240 CEM43 were achieved across 90.7–98.0% of tumor volumes for four cases. Ablations were incomplete for remaining cases, attributed to motion-affected thermometry. Thermal dose-based ablative tumor coverage agreed with viability assessment of excised tumors.Conclusions We have developed a system for delivering microwave ablation to subcutaneous tumors in small animals under MRIT guidance and demonstrated its performance in-vivo.https://www.tandfonline.com/doi/10.1080/02656736.2022.2061727Small-animal ablation systemmagnetic resonance imagingmicrowave ablationMRI thermometry |
| spellingShingle | Jan Sebek Tej B. Shrestha Matthew T. Basel Faraz Chamani Nooshin Zeinali Ivina Mali Macy Payne Sarah A. Timmerman Pegah Faridi Marla Pyle Martin O’Halloran M. Conall Dennedy Stefan H. Bossmann Punit Prakash System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance International Journal of Hyperthermia Small-animal ablation system magnetic resonance imaging microwave ablation MRI thermometry |
| title | System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance |
| title_full | System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance |
| title_fullStr | System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance |
| title_full_unstemmed | System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance |
| title_short | System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance |
| title_sort | system for delivering microwave ablation to subcutaneous tumors in small animals under high field mri thermometry guidance |
| topic | Small-animal ablation system magnetic resonance imaging microwave ablation MRI thermometry |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2022.2061727 |
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