Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach
UCMSCs were reprogrammed to iPSCs using Yamanaka factor bearing episomal plasmids. SCIKFi001-B exhibited pluripotency, had typical iPSC morphology and didn’t retain any residual episomal plasmid. Although karyotyping showed chromosomal translocation, this abnormality seemed to have little impact on...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002642 |
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| author | Marchella Tohari Ricky Sanjaya Siska Yuliana Sari Bintang Tedjobagaskara Andrian Ibnu Faisal Matheus Alvin Prawira Anggia Oktaviani Dwi Putri Naufalia Faza Harry Murti Halida P. Widyastuti |
| author_facet | Marchella Tohari Ricky Sanjaya Siska Yuliana Sari Bintang Tedjobagaskara Andrian Ibnu Faisal Matheus Alvin Prawira Anggia Oktaviani Dwi Putri Naufalia Faza Harry Murti Halida P. Widyastuti |
| author_sort | Marchella Tohari |
| collection | DOAJ |
| description | UCMSCs were reprogrammed to iPSCs using Yamanaka factor bearing episomal plasmids. SCIKFi001-B exhibited pluripotency, had typical iPSC morphology and didn’t retain any residual episomal plasmid. Although karyotyping showed chromosomal translocation, this abnormality seemed to have little impact on the functionality of SCIKFi001-B since it retained its ability to differentiate to three-germ layer. While karyotypic abnormality might negate use in therapeutic and clinical settings, this line remained a valuable educational tool for iPS cell culture techniques. Finally, our study highlighted the importance of routine karyotyping on iPSC lines as abnormal karyotypes oftentimes bear no discernible effect on cell morphology nor functionality. |
| format | Article |
| id | doaj-art-d9f9d1c3eafe4400bb1eaee2ecb8e0c1 |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-d9f9d1c3eafe4400bb1eaee2ecb8e0c12025-08-20T02:38:17ZengElsevierStem Cell Research1873-50612024-12-018110356610.1016/j.scr.2024.103566Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approachMarchella Tohari0Ricky Sanjaya1Siska Yuliana Sari2Bintang Tedjobagaskara3Andrian Ibnu Faisal4Matheus Alvin Prawira5Anggia Oktaviani Dwi Putri6Naufalia Faza7Harry Murti8Halida P. Widyastuti9Department of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, IndonesiaDepartment of Stem Cell Research and Development, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; Corresponding author.UCMSCs were reprogrammed to iPSCs using Yamanaka factor bearing episomal plasmids. SCIKFi001-B exhibited pluripotency, had typical iPSC morphology and didn’t retain any residual episomal plasmid. Although karyotyping showed chromosomal translocation, this abnormality seemed to have little impact on the functionality of SCIKFi001-B since it retained its ability to differentiate to three-germ layer. While karyotypic abnormality might negate use in therapeutic and clinical settings, this line remained a valuable educational tool for iPS cell culture techniques. Finally, our study highlighted the importance of routine karyotyping on iPSC lines as abnormal karyotypes oftentimes bear no discernible effect on cell morphology nor functionality.http://www.sciencedirect.com/science/article/pii/S1873506124002642 |
| spellingShingle | Marchella Tohari Ricky Sanjaya Siska Yuliana Sari Bintang Tedjobagaskara Andrian Ibnu Faisal Matheus Alvin Prawira Anggia Oktaviani Dwi Putri Naufalia Faza Harry Murti Halida P. Widyastuti Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach Stem Cell Research |
| title | Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach |
| title_full | Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach |
| title_fullStr | Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach |
| title_full_unstemmed | Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach |
| title_short | Generation of footprint-free human induced pluripotent stem cell line (SCIKFi001-B) from cGMP grade umbilical cord-derived mesenchymal stem cells (UC-MSCs) using episomal-plasmid based reprogramming approach |
| title_sort | generation of footprint free human induced pluripotent stem cell line scikfi001 b from cgmp grade umbilical cord derived mesenchymal stem cells uc mscs using episomal plasmid based reprogramming approach |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002642 |
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