Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments
IntroductionAlcanivorax, a typical alkane-degrading bacterium, has demonstrated the ability to utilize inorganic electron donor in some reports. However, a comprehensive analysis of its potentiality to utilize inorganic electron donor is still lacking.MethodsIn this study, genomic and phylogenetic a...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2024.1491690/full |
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| author | Zhen Chen Zhen Chen Zhen Chen Shizheng Xiang Shizheng Xiang Shizheng Xiang Yao Lu Yao Lu Yao Lu Qiliang Lai Qiliang Lai Qiliang Lai Chunming Dong Chunming Dong Chunming Dong Jianyang Li Jianyang Li Jianyang Li Guizhen Li Guizhen Li Guizhen Li Zongze Shao Zongze Shao Zongze Shao |
| author_facet | Zhen Chen Zhen Chen Zhen Chen Shizheng Xiang Shizheng Xiang Shizheng Xiang Yao Lu Yao Lu Yao Lu Qiliang Lai Qiliang Lai Qiliang Lai Chunming Dong Chunming Dong Chunming Dong Jianyang Li Jianyang Li Jianyang Li Guizhen Li Guizhen Li Guizhen Li Zongze Shao Zongze Shao Zongze Shao |
| author_sort | Zhen Chen |
| collection | DOAJ |
| description | IntroductionAlcanivorax, a typical alkane-degrading bacterium, has demonstrated the ability to utilize inorganic electron donor in some reports. However, a comprehensive analysis of its potentiality to utilize inorganic electron donor is still lacking.MethodsIn this study, genomic and phylogenetic analyzes were used to explore the potential oxidative capacity of inorganic compounds in Alcanivorax. And its functions were verified through physiological experiments.ResultsThe sulfur oxidation-related genes sqr and tsdA are prevalent and have various evolutionary origins. Potential genes for CO oxidation were present in 39 strains, whereas genes associated with iron, hydrogen, and ammonia oxidation were either rare or absent. The physiological functions of Sqr and TsdA were confirmed in six representative strains under heterotrophic conditions. Adding thiosulfate enhanced Alcanivorax growth. However, Alcanivorax bacteria perform sulfide detoxification through Sqr rather than by gaining energy via sulfide oxidation Although no strain was confirmed to be chemoautotrophs, we discovered that the two clades, A. xenomutans and A. profundimaris, can grow under conditions with very low organic matter.DiscussionThe ability to utilize inorganic compounds as a supplementary energy source and adapt to carbon oligotrophic growth may contribute to the prevalence of Alcanivorax in marine ecosystems. |
| format | Article |
| id | doaj-art-d9f9339cb2214892b11e8a965c9070ff |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-d9f9339cb2214892b11e8a965c9070ff2024-11-15T04:52:38ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-11-011110.3389/fmars.2024.14916901491690Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environmentsZhen Chen0Zhen Chen1Zhen Chen2Shizheng Xiang3Shizheng Xiang4Shizheng Xiang5Yao Lu6Yao Lu7Yao Lu8Qiliang Lai9Qiliang Lai10Qiliang Lai11Chunming Dong12Chunming Dong13Chunming Dong14Jianyang Li15Jianyang Li16Jianyang Li17Guizhen Li18Guizhen Li19Guizhen Li20Zongze Shao21Zongze Shao22Zongze Shao23Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaFujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Xiamen, ChinaIntroductionAlcanivorax, a typical alkane-degrading bacterium, has demonstrated the ability to utilize inorganic electron donor in some reports. However, a comprehensive analysis of its potentiality to utilize inorganic electron donor is still lacking.MethodsIn this study, genomic and phylogenetic analyzes were used to explore the potential oxidative capacity of inorganic compounds in Alcanivorax. And its functions were verified through physiological experiments.ResultsThe sulfur oxidation-related genes sqr and tsdA are prevalent and have various evolutionary origins. Potential genes for CO oxidation were present in 39 strains, whereas genes associated with iron, hydrogen, and ammonia oxidation were either rare or absent. The physiological functions of Sqr and TsdA were confirmed in six representative strains under heterotrophic conditions. Adding thiosulfate enhanced Alcanivorax growth. However, Alcanivorax bacteria perform sulfide detoxification through Sqr rather than by gaining energy via sulfide oxidation Although no strain was confirmed to be chemoautotrophs, we discovered that the two clades, A. xenomutans and A. profundimaris, can grow under conditions with very low organic matter.DiscussionThe ability to utilize inorganic compounds as a supplementary energy source and adapt to carbon oligotrophic growth may contribute to the prevalence of Alcanivorax in marine ecosystems.https://www.frontiersin.org/articles/10.3389/fmars.2024.1491690/fullAlcanivoraxchemolithotrophiccarbon oligotrophicsulfur oxidationTsdASqr |
| spellingShingle | Zhen Chen Zhen Chen Zhen Chen Shizheng Xiang Shizheng Xiang Shizheng Xiang Yao Lu Yao Lu Yao Lu Qiliang Lai Qiliang Lai Qiliang Lai Chunming Dong Chunming Dong Chunming Dong Jianyang Li Jianyang Li Jianyang Li Guizhen Li Guizhen Li Guizhen Li Zongze Shao Zongze Shao Zongze Shao Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments Frontiers in Marine Science Alcanivorax chemolithotrophic carbon oligotrophic sulfur oxidation TsdA Sqr |
| title | Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments |
| title_full | Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments |
| title_fullStr | Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments |
| title_full_unstemmed | Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments |
| title_short | Adaptation mechanisms of Alcanivorax facilitating its predominance in marine environments |
| title_sort | adaptation mechanisms of alcanivorax facilitating its predominance in marine environments |
| topic | Alcanivorax chemolithotrophic carbon oligotrophic sulfur oxidation TsdA Sqr |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1491690/full |
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