Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome
The present study aimed to evaluate the expression of CD80 and CD18 in subpopulations of peripheral blood leukocytes and oxidative kidney damage in rats with nephrotic syndrome (NS) induced by doxorubicin (Dox) in comparison to control animals at different time points. Male adult Wistar rats were su...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/209764 |
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| author | Wagner de Fátima Pereira Gustavo Eustáquio Alvim Brito-Melo Cláudia Martins Carneiro Dirceu de Sousa Melo Karine Beatriz Costa Fábio Lourenço Tadeu Guimarães Etel Rocha-Vieira Érica Leandro Marciano Vieira Ana Cristina Simões e Silva |
| author_facet | Wagner de Fátima Pereira Gustavo Eustáquio Alvim Brito-Melo Cláudia Martins Carneiro Dirceu de Sousa Melo Karine Beatriz Costa Fábio Lourenço Tadeu Guimarães Etel Rocha-Vieira Érica Leandro Marciano Vieira Ana Cristina Simões e Silva |
| author_sort | Wagner de Fátima Pereira |
| collection | DOAJ |
| description | The present study aimed to evaluate the expression of CD80 and CD18 in subpopulations of peripheral blood leukocytes and oxidative kidney damage in rats with nephrotic syndrome (NS) induced by doxorubicin (Dox) in comparison to control animals at different time points. Male adult Wistar rats were submitted to 24-hour urine and blood collection for biochemical and immunological analysis at 7, 14, 21, and 28 days after Dox injection. After euthanasia, the kidneys were removed for histological analysis and the evaluation of oxidative stress. The phenotypic characterization of leukocytes was performed using flow cytometry. Dox-injected animals exhibited increased CD18 expression in cytotoxic T lymphocytes, NK cells, and monocytes and high CD80 expression in monocytes. Kidney oxidative damage was positively correlated with CD80 expression in monocytes and serum levels of creatinine. These results suggest that phagocytic and cytotoxic cells are preferentially recruited to the tissue injury site, which may contribute to kidney dysfunction in this animal model of NS. The blockade of integrin and costimulatory molecules may provide new therapeutic opportunities for NS. |
| format | Article |
| id | doaj-art-d9f5e6523d8945d4b5be41b03ffebe41 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d9f5e6523d8945d4b5be41b03ffebe412025-08-20T03:24:25ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/209764209764Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic SyndromeWagner de Fátima Pereira0Gustavo Eustáquio Alvim Brito-Melo1Cláudia Martins Carneiro2Dirceu de Sousa Melo3Karine Beatriz Costa4Fábio Lourenço Tadeu Guimarães5Etel Rocha-Vieira6Érica Leandro Marciano Vieira7Ana Cristina Simões e Silva8Immunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilImmunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilImmunopathology Laboratory, Center for Research in Biological Sciences (LIMP/NUPEB), Universidade Federal de Ouro Preto (UFOP), 30140-081 Ouro Preto, MG, BrazilImmunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilImmunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilImmunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilImmunology Laboratory, Integrated Health Research Center, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), 38610-000 Diamantina, MG, BrazilInterdisciplinary Laboratory of Medical Investigation, Pediatric Nephrology Unit, Universidade Federal de Minas Gerais (UFMG), 30130-100 Belo Horizonte, MG, BrazilInterdisciplinary Laboratory of Medical Investigation, Pediatric Nephrology Unit, Universidade Federal de Minas Gerais (UFMG), 30130-100 Belo Horizonte, MG, BrazilThe present study aimed to evaluate the expression of CD80 and CD18 in subpopulations of peripheral blood leukocytes and oxidative kidney damage in rats with nephrotic syndrome (NS) induced by doxorubicin (Dox) in comparison to control animals at different time points. Male adult Wistar rats were submitted to 24-hour urine and blood collection for biochemical and immunological analysis at 7, 14, 21, and 28 days after Dox injection. After euthanasia, the kidneys were removed for histological analysis and the evaluation of oxidative stress. The phenotypic characterization of leukocytes was performed using flow cytometry. Dox-injected animals exhibited increased CD18 expression in cytotoxic T lymphocytes, NK cells, and monocytes and high CD80 expression in monocytes. Kidney oxidative damage was positively correlated with CD80 expression in monocytes and serum levels of creatinine. These results suggest that phagocytic and cytotoxic cells are preferentially recruited to the tissue injury site, which may contribute to kidney dysfunction in this animal model of NS. The blockade of integrin and costimulatory molecules may provide new therapeutic opportunities for NS.http://dx.doi.org/10.1155/2015/209764 |
| spellingShingle | Wagner de Fátima Pereira Gustavo Eustáquio Alvim Brito-Melo Cláudia Martins Carneiro Dirceu de Sousa Melo Karine Beatriz Costa Fábio Lourenço Tadeu Guimarães Etel Rocha-Vieira Érica Leandro Marciano Vieira Ana Cristina Simões e Silva Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome Mediators of Inflammation |
| title | Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome |
| title_full | Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome |
| title_fullStr | Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome |
| title_full_unstemmed | Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome |
| title_short | Increased Migratory and Activation Cell Markers of Peripheral Blood Lymphocytes in an Experimental Model of Nephrotic Syndrome |
| title_sort | increased migratory and activation cell markers of peripheral blood lymphocytes in an experimental model of nephrotic syndrome |
| url | http://dx.doi.org/10.1155/2015/209764 |
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