Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai

To investigate the changes and interactions between microbial communities and flavor compounds during the fermentation process of Yibin Yacai, high-throughput sequencing technology and gas chromatography-mass spectrometry were applied to determine the microbial communities and flavor compounds durin...

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Main Authors: Xueling CHEN, Kunyi LIU, Qi WANG, Xinxin NIU, Cheng LIAO, Jiangshan AN, Yunong ZHOU, Zhuo CHEN, Sisi GUAN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-07-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024110263
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author Xueling CHEN
Kunyi LIU
Qi WANG
Xinxin NIU
Cheng LIAO
Jiangshan AN
Yunong ZHOU
Zhuo CHEN
Sisi GUAN
author_facet Xueling CHEN
Kunyi LIU
Qi WANG
Xinxin NIU
Cheng LIAO
Jiangshan AN
Yunong ZHOU
Zhuo CHEN
Sisi GUAN
author_sort Xueling CHEN
collection DOAJ
description To investigate the changes and interactions between microbial communities and flavor compounds during the fermentation process of Yibin Yacai, high-throughput sequencing technology and gas chromatography-mass spectrometry were applied to determine the microbial communities and flavor compounds during the fermentation process of Yibin Yacai, while, Spearman correlation was used to explore the relationship between microbial communities and characteristic flavor compounds. The results showed that the dominant microorganisms gradually changed from Pediococcus, Levilactobacillus, Torulaspora and Zygosaccharomyces in the early stage of fermentation to Levilactobacillus, Companilactobacillus, Zygosaccharomyces and Millerozyma. On the 7th month of fermentation, they further changed to Ligilactobacillus, Loigolactobacillus and Zygosaccharomyces, while, Levilactobacillus, Loigolactobacillus, Companilactobacillus, Zygosaccharomyces and Candida became the dominant microorganisms in the later stage of fermentation. Meanwhile, 924 flavor compounds were detected in the fermented samples of Yibin Yacai, among which 33 were characteristic flavor compounds (VIP>1, ROAV>1) during the fermentation process of Yibin Yacai. In addition, Loigolactobacillus, Zygosaccharomyces and Torulaspora were extremely significantly correlated with four terpenoids, including myrcene, 2-methylisoborneol, D-limonene, o-cymene, two phenols, including 4-ethyl-phenol, 4-ethyl-2-methoxy-phenol, and 2-dodecanone, (6Z)-nonen-1-ol, 2-acetyl-3-methylpyrazine, 2-methyl-benzaldehyde (P<0.01). Overall, these findings provide a theoretical basis for utilizing microorganisms to regulate the production and improve fermentation processes of Yibin Yacai.
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spelling doaj-art-d9f06fe85c0843448df511c84f50a62e2025-08-20T03:23:56ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-07-014613364510.13386/j.issn1002-0306.20241102632024110263-13Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin YacaiXueling CHEN0Kunyi LIU1Qi WANG2Xinxin NIU3Cheng LIAO4Jiangshan AN5Yunong ZHOU6Zhuo CHEN7Sisi GUAN8School of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaSchool of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaSchool of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaSchool of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaNanguang Primary School in Xuzhou District of Yibin City, Yibin 644002, ChinaCollege of Tea Science, Yunnan Agricultural University, Kunming 650201, ChinaSichuan Yibin Suimi Yacai Co., Ltd., Yibin 644011, ChinaSchool of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaSchool of Wuliangye Technology and Food Engineering & Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin Vocational and Technical College, Yibin 644000, ChinaTo investigate the changes and interactions between microbial communities and flavor compounds during the fermentation process of Yibin Yacai, high-throughput sequencing technology and gas chromatography-mass spectrometry were applied to determine the microbial communities and flavor compounds during the fermentation process of Yibin Yacai, while, Spearman correlation was used to explore the relationship between microbial communities and characteristic flavor compounds. The results showed that the dominant microorganisms gradually changed from Pediococcus, Levilactobacillus, Torulaspora and Zygosaccharomyces in the early stage of fermentation to Levilactobacillus, Companilactobacillus, Zygosaccharomyces and Millerozyma. On the 7th month of fermentation, they further changed to Ligilactobacillus, Loigolactobacillus and Zygosaccharomyces, while, Levilactobacillus, Loigolactobacillus, Companilactobacillus, Zygosaccharomyces and Candida became the dominant microorganisms in the later stage of fermentation. Meanwhile, 924 flavor compounds were detected in the fermented samples of Yibin Yacai, among which 33 were characteristic flavor compounds (VIP>1, ROAV>1) during the fermentation process of Yibin Yacai. In addition, Loigolactobacillus, Zygosaccharomyces and Torulaspora were extremely significantly correlated with four terpenoids, including myrcene, 2-methylisoborneol, D-limonene, o-cymene, two phenols, including 4-ethyl-phenol, 4-ethyl-2-methoxy-phenol, and 2-dodecanone, (6Z)-nonen-1-ol, 2-acetyl-3-methylpyrazine, 2-methyl-benzaldehyde (P<0.01). Overall, these findings provide a theoretical basis for utilizing microorganisms to regulate the production and improve fermentation processes of Yibin Yacai.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024110263yibin yacaifermentationmicrobial communityvolatile flavor compoundcorrelation analysis
spellingShingle Xueling CHEN
Kunyi LIU
Qi WANG
Xinxin NIU
Cheng LIAO
Jiangshan AN
Yunong ZHOU
Zhuo CHEN
Sisi GUAN
Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
Shipin gongye ke-ji
yibin yacai
fermentation
microbial community
volatile flavor compound
correlation analysis
title Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
title_full Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
title_fullStr Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
title_full_unstemmed Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
title_short Changes and Correlation between Microbial Communities and Flavor Compounds during the Fermentation Process of Yibin Yacai
title_sort changes and correlation between microbial communities and flavor compounds during the fermentation process of yibin yacai
topic yibin yacai
fermentation
microbial community
volatile flavor compound
correlation analysis
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024110263
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