The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer
This study aims to unveil the marine invertebrate <i>Sidnyum elegans</i>, a <i>Mediterranean ascidian</i>, as a natural resource for the early development of new treatments for triple-negative breast cancer (TNBC). Nine different fractions obtained via medium-pressure liquid...
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2025-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/5/195 |
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| author | Marcello Casertano Camilla Esposito Ivana Bello Martina Barile Luana Izzo Emma Mitidieri Raffaella Sorrentino Marialuisa Menna Elisabetta Panza Concetta Imperatore Roberta d’Emmanuele di Villa Bianca |
| author_facet | Marcello Casertano Camilla Esposito Ivana Bello Martina Barile Luana Izzo Emma Mitidieri Raffaella Sorrentino Marialuisa Menna Elisabetta Panza Concetta Imperatore Roberta d’Emmanuele di Villa Bianca |
| author_sort | Marcello Casertano |
| collection | DOAJ |
| description | This study aims to unveil the marine invertebrate <i>Sidnyum elegans</i>, a <i>Mediterranean ascidian</i>, as a natural resource for the early development of new treatments for triple-negative breast cancer (TNBC). Nine different fractions obtained via medium-pressure liquid chromatography (MPLC) of the butanol-soluble material of the ascidian were evaluated in proliferating MDA-MB-231 cells in a range of 10–50 µg/mL. Among them, the SEB-5 fraction was found to be the most effective in reducing cell proliferation and concomitantly inducing apoptosis, revealed via MTT assay and FACS analysis using Annexin V/PI dual staining. Furthermore, we investigated the effect of this fraction on cell cycle phases, revealing that SEB-5 can arrest the cells in the G0/G1 phase. This latter effect was then confirmed via transcriptomic analysis, showing that treatment with SEB-5 reduced the expression of cyclinB1, CDC25a, and CDK1. Finally, to evaluate the potential antimetastatic effect of SEB-5, a wound-healing assay was performed showing the ability of SEB-5 to reduce MDA-MB-231 cell migration. The chemical characterization of SEB-5 components was performed using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS/MS) and nuclear magnetic resonance (NMR) spectroscopy. This analysis revealed the presence of a terpenoid and polyketide-like compounds, including the alkyl sulfate <b>1</b> and phosphoeleganin <b>2</b>, along with three novel phosphoeleganin-related products <b>3</b>–<b>5</b>. |
| format | Article |
| id | doaj-art-d9edea46bb224734a274b6849a23d66c |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-d9edea46bb224734a274b6849a23d66c2025-08-20T01:56:17ZengMDPI AGMarine Drugs1660-33972025-04-0123519510.3390/md23050195The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast CancerMarcello Casertano0Camilla Esposito1Ivana Bello2Martina Barile3Luana Izzo4Emma Mitidieri5Raffaella Sorrentino6Marialuisa Menna7Elisabetta Panza8Concetta Imperatore9Roberta d’Emmanuele di Villa Bianca10Department of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131 Naples, ItalyThis study aims to unveil the marine invertebrate <i>Sidnyum elegans</i>, a <i>Mediterranean ascidian</i>, as a natural resource for the early development of new treatments for triple-negative breast cancer (TNBC). Nine different fractions obtained via medium-pressure liquid chromatography (MPLC) of the butanol-soluble material of the ascidian were evaluated in proliferating MDA-MB-231 cells in a range of 10–50 µg/mL. Among them, the SEB-5 fraction was found to be the most effective in reducing cell proliferation and concomitantly inducing apoptosis, revealed via MTT assay and FACS analysis using Annexin V/PI dual staining. Furthermore, we investigated the effect of this fraction on cell cycle phases, revealing that SEB-5 can arrest the cells in the G0/G1 phase. This latter effect was then confirmed via transcriptomic analysis, showing that treatment with SEB-5 reduced the expression of cyclinB1, CDC25a, and CDK1. Finally, to evaluate the potential antimetastatic effect of SEB-5, a wound-healing assay was performed showing the ability of SEB-5 to reduce MDA-MB-231 cell migration. The chemical characterization of SEB-5 components was performed using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS/MS) and nuclear magnetic resonance (NMR) spectroscopy. This analysis revealed the presence of a terpenoid and polyketide-like compounds, including the alkyl sulfate <b>1</b> and phosphoeleganin <b>2</b>, along with three novel phosphoeleganin-related products <b>3</b>–<b>5</b>.https://www.mdpi.com/1660-3397/23/5/195triple-negative breast cancermarine invertebratesascidianstunicates<i>Sidnyum elegans</i>marine natural products |
| spellingShingle | Marcello Casertano Camilla Esposito Ivana Bello Martina Barile Luana Izzo Emma Mitidieri Raffaella Sorrentino Marialuisa Menna Elisabetta Panza Concetta Imperatore Roberta d’Emmanuele di Villa Bianca The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer Marine Drugs triple-negative breast cancer marine invertebrates ascidians tunicates <i>Sidnyum elegans</i> marine natural products |
| title | The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer |
| title_full | The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer |
| title_fullStr | The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer |
| title_full_unstemmed | The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer |
| title_short | The Mediterranean Sea on the Bench: Unveiling the Marine Invertebrate <i>Sidnyum elegans</i> as a Source of Novel Promising Therapeutic Tools Against Triple-Negative Breast Cancer |
| title_sort | mediterranean sea on the bench unveiling the marine invertebrate i sidnyum elegans i as a source of novel promising therapeutic tools against triple negative breast cancer |
| topic | triple-negative breast cancer marine invertebrates ascidians tunicates <i>Sidnyum elegans</i> marine natural products |
| url | https://www.mdpi.com/1660-3397/23/5/195 |
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