Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin
Abstract Hypostomus is a remarkable group within Siluriformes due to its high species richness, pronounced morphological and chromosomal variability, which complicates the process of species identification. In this context, the aim of this study was to identify possible interpopulational chromosomal...
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Sociedade Brasileira de Ictiologia
2025-06-01
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| Series: | Neotropical Ichthyology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000200209&lng=en&tlng=en |
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| author | Lígia Carla Balini Rafael Fernando de Melo Ana Luiza de Brito Portela-Castro Cláudio Henrique Zawadzki Marcos Otávio Ribeiro Carlos Alexandre Fernandes Luciana Andreia Borin-Carvalho |
| author_facet | Lígia Carla Balini Rafael Fernando de Melo Ana Luiza de Brito Portela-Castro Cláudio Henrique Zawadzki Marcos Otávio Ribeiro Carlos Alexandre Fernandes Luciana Andreia Borin-Carvalho |
| author_sort | Lígia Carla Balini |
| collection | DOAJ |
| description | Abstract Hypostomus is a remarkable group within Siluriformes due to its high species richness, pronounced morphological and chromosomal variability, which complicates the process of species identification. In this context, the aim of this study was to identify possible interpopulational chromosomal markers that could significantly contribute to our understanding of the differentiation in H. strigaticeps of distinct tributaries. Two H. strigaticeps populations from the upper Paraná River basin were analyzed using cytogenetic methods.Our results revealed interpopulation variations in H. strigaticeps, despite limited sampling of the Ivaí River population,particularly in the number of 18S and 5S rDNA sites. Moreover, the individuals from both populations exhibited the same chromosome count (2n = 72) and fundamental number (FN = 108), as well as identical chromosome morphology and similarities in the distribution of constitutive heterochromatin. The transposable elements (TEs) Rex1 and Rex3 were found scattered along the chromosomes, with a higher concentration of Rex3 elements in pairs carrying rDNA. Our findings provide novel insights into a possible involvement of TEs in the variability of rDNA distribution patterns, contributing to karyotypic diversification in H. strigaticeps. |
| format | Article |
| id | doaj-art-d9ec67a79ed54edbba91ae4df0ea363d |
| institution | Kabale University |
| issn | 1982-0224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj-art-d9ec67a79ed54edbba91ae4df0ea363d2025-08-20T03:28:17ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242025-06-0123210.1590/1982-0224-2024-0061Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basinLígia Carla Balinihttps://orcid.org/0009-0008-1502-8408Rafael Fernando de Melohttps://orcid.org/0000-0002-6975-6346Ana Luiza de Brito Portela-Castrohttps://orcid.org/0000-0002-0881-0345Cláudio Henrique Zawadzkihttps://orcid.org/0000-0002-5642-4908Marcos Otávio Ribeirohttps://orcid.org/0000-0001-8914-6257Carlos Alexandre Fernandeshttps://orcid.org/0000-0002-9905-1507Luciana Andreia Borin-Carvalhohttps://orcid.org/0000-0003-1990-4803Abstract Hypostomus is a remarkable group within Siluriformes due to its high species richness, pronounced morphological and chromosomal variability, which complicates the process of species identification. In this context, the aim of this study was to identify possible interpopulational chromosomal markers that could significantly contribute to our understanding of the differentiation in H. strigaticeps of distinct tributaries. Two H. strigaticeps populations from the upper Paraná River basin were analyzed using cytogenetic methods.Our results revealed interpopulation variations in H. strigaticeps, despite limited sampling of the Ivaí River population,particularly in the number of 18S and 5S rDNA sites. Moreover, the individuals from both populations exhibited the same chromosome count (2n = 72) and fundamental number (FN = 108), as well as identical chromosome morphology and similarities in the distribution of constitutive heterochromatin. The transposable elements (TEs) Rex1 and Rex3 were found scattered along the chromosomes, with a higher concentration of Rex3 elements in pairs carrying rDNA. Our findings provide novel insights into a possible involvement of TEs in the variability of rDNA distribution patterns, contributing to karyotypic diversification in H. strigaticeps.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000200209&lng=en&tlng=enHeterochromatinKaryotype evolutionNORsRetrotransposonsTransposable elements |
| spellingShingle | Lígia Carla Balini Rafael Fernando de Melo Ana Luiza de Brito Portela-Castro Cláudio Henrique Zawadzki Marcos Otávio Ribeiro Carlos Alexandre Fernandes Luciana Andreia Borin-Carvalho Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin Neotropical Ichthyology Heterochromatin Karyotype evolution NORs Retrotransposons Transposable elements |
| title | Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin |
| title_full | Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin |
| title_fullStr | Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin |
| title_full_unstemmed | Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin |
| title_short | Polymorphism of 5S and 18S ribosomal DNA in a conserved karyotype of the two Hypostomus strigaticeps (Siluriformes: Loricariidae) populations from the upper Paraná River basin |
| title_sort | polymorphism of 5s and 18s ribosomal dna in a conserved karyotype of the two hypostomus strigaticeps siluriformes loricariidae populations from the upper parana river basin |
| topic | Heterochromatin Karyotype evolution NORs Retrotransposons Transposable elements |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000200209&lng=en&tlng=en |
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