Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor

Abstract The endogenous opioid system provides powerful control over emotions, nociception, and motivation among many other fundamental nervous system functions. Its major components include a panel of opioid peptides that activate four canonical inhibitory opioid receptors. However, its regulatory...

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Main Authors: Xiaona Li, Nathan D. Winters, Shubhi Pandey, Colten Lankford, Hannah M. Stoveken, Emery Smith, Chu-Ting Chang, Stefano Zucca, Louis Scampavia, Timothy Spicer, Kirill A. Martemyanov
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62133-x
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author Xiaona Li
Nathan D. Winters
Shubhi Pandey
Colten Lankford
Hannah M. Stoveken
Emery Smith
Chu-Ting Chang
Stefano Zucca
Louis Scampavia
Timothy Spicer
Kirill A. Martemyanov
author_facet Xiaona Li
Nathan D. Winters
Shubhi Pandey
Colten Lankford
Hannah M. Stoveken
Emery Smith
Chu-Ting Chang
Stefano Zucca
Louis Scampavia
Timothy Spicer
Kirill A. Martemyanov
author_sort Xiaona Li
collection DOAJ
description Abstract The endogenous opioid system provides powerful control over emotions, nociception, and motivation among many other fundamental nervous system functions. Its major components include a panel of opioid peptides that activate four canonical inhibitory opioid receptors. However, its regulatory principles are not fully understood including the existence of additional receptors and other elements. In this study we report the identification of a receptor for the opioid peptide dynorphin. By conducting a screen of a custom library of neuropeptides, we found that orphan receptor GPR139 binds to and is activated by a series of dynorphin peptides. Unlike other opioid receptors, GPR139 couples to Gq/11 and avoids β-arrestin, providing excitatory signaling that homeostatically scales the inhibitory response of neurons to dynorphin. This introduces a non-canonical dynorphin receptor as an essential component of the opioid system.
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issn 2041-1723
language English
publishDate 2025-07-01
publisher Nature Portfolio
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series Nature Communications
spelling doaj-art-d9cd21f897d6444c90f12f36fc2713862025-08-20T03:43:14ZengNature PortfolioNature Communications2041-17232025-07-0116111610.1038/s41467-025-62133-xHomeostatic scaling of dynorphin signaling by a non-canonical opioid receptorXiaona Li0Nathan D. Winters1Shubhi Pandey2Colten Lankford3Hannah M. Stoveken4Emery Smith5Chu-Ting Chang6Stefano Zucca7Louis Scampavia8Timothy Spicer9Kirill A. Martemyanov10Department of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyDepartment of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & TechnologyAbstract The endogenous opioid system provides powerful control over emotions, nociception, and motivation among many other fundamental nervous system functions. Its major components include a panel of opioid peptides that activate four canonical inhibitory opioid receptors. However, its regulatory principles are not fully understood including the existence of additional receptors and other elements. In this study we report the identification of a receptor for the opioid peptide dynorphin. By conducting a screen of a custom library of neuropeptides, we found that orphan receptor GPR139 binds to and is activated by a series of dynorphin peptides. Unlike other opioid receptors, GPR139 couples to Gq/11 and avoids β-arrestin, providing excitatory signaling that homeostatically scales the inhibitory response of neurons to dynorphin. This introduces a non-canonical dynorphin receptor as an essential component of the opioid system.https://doi.org/10.1038/s41467-025-62133-x
spellingShingle Xiaona Li
Nathan D. Winters
Shubhi Pandey
Colten Lankford
Hannah M. Stoveken
Emery Smith
Chu-Ting Chang
Stefano Zucca
Louis Scampavia
Timothy Spicer
Kirill A. Martemyanov
Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
Nature Communications
title Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
title_full Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
title_fullStr Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
title_full_unstemmed Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
title_short Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor
title_sort homeostatic scaling of dynorphin signaling by a non canonical opioid receptor
url https://doi.org/10.1038/s41467-025-62133-x
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