In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus
Xylooligosaccharides (XOS) produced from pinewood sawdust and sugarcane bagasse xylan using crude xylanase from the probiotic Bacillus licheniformis were used to evaluate their ability to stimulate the growth of Lactobacillus species, as well as their antibacterial activity against multidrug-resista...
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Taylor & Francis Group
2025-12-01
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| Series: | Cogent Food & Agriculture |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2025.2546991 |
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| author | Claudious Gufe Tatenda Chijaka Tinashe Muteveri Peter Katsande Benard Mbonjani Prosper Jambwa |
| author_facet | Claudious Gufe Tatenda Chijaka Tinashe Muteveri Peter Katsande Benard Mbonjani Prosper Jambwa |
| author_sort | Claudious Gufe |
| collection | DOAJ |
| description | Xylooligosaccharides (XOS) produced from pinewood sawdust and sugarcane bagasse xylan using crude xylanase from the probiotic Bacillus licheniformis were used to evaluate their ability to stimulate the growth of Lactobacillus species, as well as their antibacterial activity against multidrug-resistant Staphylococcus aureus (MDR-SA) isolated from cows with recurrent mastitis. The ability of the two XOS products to stimulate probiotic growth was evaluated using selected Lactobacillus strains, while the antibacterial activity of the postbiotics against MDR-SA was determined using the agar well diffusion method. Most of the S. aureus isolates (75%) had a 90% antibiotic-resistant rate. The XOS products stimulated the growth of Lactobacillus species, with the probiotic cultures growing faster when sugarcane bagasse XOS was used as a carbon source compared to pinewood sawdust XOS. Lactobacillus plantarum has a higher specific growth rate than Lactobacillus acidophilus and Lactobacillus casei. Cell-free supernatants of probiotic bacteria grown in a medium containing XOS as the sole carbon source exhibited weak to strong antibacterial activity against MDR-SA, with L. plantarum supernatants from the two lignocellulosic biomasses showing the most inhibition, followed by L. acidophilus and L. casei. The study showed that XOS generated from xylan extracted from the two biomasses has the potential to promote the growth of beneficial Lactobacillus species and that XOS plus probiotic combinations are prospective antibacterial agents against mastitogenic MDR-SA strains. |
| format | Article |
| id | doaj-art-d9c9f09473f74409a3d2fe407a0ffcee |
| institution | Kabale University |
| issn | 2331-1932 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj-art-d9c9f09473f74409a3d2fe407a0ffcee2025-08-20T03:47:10ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322025-12-0111110.1080/23311932.2025.2546991In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureusClaudious Gufe0Tatenda Chijaka1Tinashe Muteveri2Peter Katsande3Benard Mbonjani4Prosper Jambwa5Department of Veterinary Technical Services, Central Veterinary Laboratories, Harare, ZimbabweDepartment of Applied Biosciences and Biotechnology, Faculty of Science and Technology, Midlands State University, Gweru, ZimbabweDepartment of Applied Biosciences and Biotechnology, Faculty of Science and Technology, Midlands State University, Gweru, ZimbabweDepartment of Veterinary Technical Services, Central Veterinary Laboratories, Harare, ZimbabweDepartment of Veterinary Technical Services, Central Veterinary Laboratories, Harare, ZimbabweDepartment of Veterinary Biosciences, Faculty of Veterinary Science, University of Zimbabwe, Harare, ZimbabweXylooligosaccharides (XOS) produced from pinewood sawdust and sugarcane bagasse xylan using crude xylanase from the probiotic Bacillus licheniformis were used to evaluate their ability to stimulate the growth of Lactobacillus species, as well as their antibacterial activity against multidrug-resistant Staphylococcus aureus (MDR-SA) isolated from cows with recurrent mastitis. The ability of the two XOS products to stimulate probiotic growth was evaluated using selected Lactobacillus strains, while the antibacterial activity of the postbiotics against MDR-SA was determined using the agar well diffusion method. Most of the S. aureus isolates (75%) had a 90% antibiotic-resistant rate. The XOS products stimulated the growth of Lactobacillus species, with the probiotic cultures growing faster when sugarcane bagasse XOS was used as a carbon source compared to pinewood sawdust XOS. Lactobacillus plantarum has a higher specific growth rate than Lactobacillus acidophilus and Lactobacillus casei. Cell-free supernatants of probiotic bacteria grown in a medium containing XOS as the sole carbon source exhibited weak to strong antibacterial activity against MDR-SA, with L. plantarum supernatants from the two lignocellulosic biomasses showing the most inhibition, followed by L. acidophilus and L. casei. The study showed that XOS generated from xylan extracted from the two biomasses has the potential to promote the growth of beneficial Lactobacillus species and that XOS plus probiotic combinations are prospective antibacterial agents against mastitogenic MDR-SA strains.https://www.tandfonline.com/doi/10.1080/23311932.2025.2546991Xylooligosaccharidessugarcane bagassepinewood sawdustmastitogenic multidrug-resistant Staphylococcus aureusLactobacillus speciesBiochemistry |
| spellingShingle | Claudious Gufe Tatenda Chijaka Tinashe Muteveri Peter Katsande Benard Mbonjani Prosper Jambwa In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus Cogent Food & Agriculture Xylooligosaccharides sugarcane bagasse pinewood sawdust mastitogenic multidrug-resistant Staphylococcus aureus Lactobacillus species Biochemistry |
| title | In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus |
| title_full | In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus |
| title_fullStr | In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus |
| title_full_unstemmed | In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus |
| title_short | In vitro biological effects of pinewood sawdust and sugarcane bagasse-derived xylooligosaccharides on Lactobacillus biomass and antibacterial properties of its postbiotics on multidrug-resistant mastitogenic Staphylococcus aureus |
| title_sort | in vitro biological effects of pinewood sawdust and sugarcane bagasse derived xylooligosaccharides on lactobacillus biomass and antibacterial properties of its postbiotics on multidrug resistant mastitogenic staphylococcus aureus |
| topic | Xylooligosaccharides sugarcane bagasse pinewood sawdust mastitogenic multidrug-resistant Staphylococcus aureus Lactobacillus species Biochemistry |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2025.2546991 |
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