Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87
Objective To prepare a multifunctional mesoporous silica-based nanocarrier system(MMSN@Gem) with gemcitabine and investigate its effect on bladder cancer cells BIU-87. Methods The multifunctional mesoporous silica-based nano drug-carrying system was prepared by a modified method. Transmission electr...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-06-01
|
| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-777.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106757585567744 |
|---|---|
| author | WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng |
| author_facet | WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng |
| author_sort | WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng |
| collection | DOAJ |
| description | Objective To prepare a multifunctional mesoporous silica-based nanocarrier system(MMSN@Gem) with gemcitabine and investigate its effect on bladder cancer cells BIU-87. Methods The multifunctional mesoporous silica-based nano drug-carrying system was prepared by a modified method. Transmission electron microscopy, high-performance liquid chromatography, and thermal imaging were used to characterize the morphology, drug-carrying and photothermal properties of MMSN@Gem. The effect of MMSN@Gem on BIU-87 bladder cancer cells was detected by in vitro experiments. Results MMSN@Gem exhibited a well-defined spherical morphology with an average particle size of(102.48±1.03)nm with a drug loading capacity of 25.04%±0.17%, and an average zeta potential of(-24.84±0.07)mV. The photothermal conversion efficiency was 40.7% which significantly enhanced the release of Gem under near-infrared irradiation. In vitro studies showed that MMSN@Gem significantly inhibited BIU-87 cell activity, induced apoptosis of BIU-87 cells, reduced migration and invasion ability, and enhanced its uptake by BIU-87 cells. Conclusions MMSN@Gem exhibits excellent photothermal properties, enhances cellular uptake efficiency, inhibits the proliferation and migration of BIU-87 cells, and promotes apoptosis, providing a promising drug delivery system for the clinical treatment of bladder cancer. |
| format | Article |
| id | doaj-art-d9c729efee3c4037b9b934ba50ae39b4 |
| institution | OA Journals |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-d9c729efee3c4037b9b934ba50ae39b42025-08-20T02:38:45ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-06-0145677778510.16352/j.issn.1001-6325.2025.06.0777Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng0Department of Urology, Wenzhou Central Hospital,Wenzhou 325000, ChinaObjective To prepare a multifunctional mesoporous silica-based nanocarrier system(MMSN@Gem) with gemcitabine and investigate its effect on bladder cancer cells BIU-87. Methods The multifunctional mesoporous silica-based nano drug-carrying system was prepared by a modified method. Transmission electron microscopy, high-performance liquid chromatography, and thermal imaging were used to characterize the morphology, drug-carrying and photothermal properties of MMSN@Gem. The effect of MMSN@Gem on BIU-87 bladder cancer cells was detected by in vitro experiments. Results MMSN@Gem exhibited a well-defined spherical morphology with an average particle size of(102.48±1.03)nm with a drug loading capacity of 25.04%±0.17%, and an average zeta potential of(-24.84±0.07)mV. The photothermal conversion efficiency was 40.7% which significantly enhanced the release of Gem under near-infrared irradiation. In vitro studies showed that MMSN@Gem significantly inhibited BIU-87 cell activity, induced apoptosis of BIU-87 cells, reduced migration and invasion ability, and enhanced its uptake by BIU-87 cells. Conclusions MMSN@Gem exhibits excellent photothermal properties, enhances cellular uptake efficiency, inhibits the proliferation and migration of BIU-87 cells, and promotes apoptosis, providing a promising drug delivery system for the clinical treatment of bladder cancer.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-777.pdfgemcitabine|nano co-loading system|bladder tumour|mesoporous silica |
| spellingShingle | WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 Jichu yixue yu linchuang gemcitabine|nano co-loading system|bladder tumour|mesoporous silica |
| title | Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 |
| title_full | Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 |
| title_fullStr | Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 |
| title_full_unstemmed | Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 |
| title_short | Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87 |
| title_sort | construction of mmsn gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line biu 87 |
| topic | gemcitabine|nano co-loading system|bladder tumour|mesoporous silica |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-777.pdf |
| work_keys_str_mv | AT wangdayalizhijiazhaodeweichenximeng constructionofmmsngemanditsinhibitionofproliferationandpromotionofapoptosisinhumanbladdercancercelllinebiu87 |