Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson
Bocconia arborea S. Watson (B. arborea) belongs to the Papaveraceae family and is used in folk medicine because of its depressant effect on the central nervous system. However, its benefits on cerebral paroxysmal activity as an anticonvulsant treatment remain unknown. This study aimed to evaluate th...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025010825 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849721085843472384 |
|---|---|
| author | José David González-Gómez Samuel Estrada-Soto Emmanuel Gaona-Tovar María Eva González-Trujano Benjamín Villasana-Salazar Hugo Fernando Narváez-González David Martínez-Vargas |
| author_facet | José David González-Gómez Samuel Estrada-Soto Emmanuel Gaona-Tovar María Eva González-Trujano Benjamín Villasana-Salazar Hugo Fernando Narváez-González David Martínez-Vargas |
| author_sort | José David González-Gómez |
| collection | DOAJ |
| description | Bocconia arborea S. Watson (B. arborea) belongs to the Papaveraceae family and is used in folk medicine because of its depressant effect on the central nervous system. However, its benefits on cerebral paroxysmal activity as an anticonvulsant treatment remain unknown. This study aimed to evaluate the anticonvulsant effect of B. arborea and one of its abundant isolated metabolites dihydrosanguinarine (DHS) by means of experimental models of seizures in mice simultaneously to an electrocorticographic (ECoG) analysis. B. arborea was prepared as extracts with different polarity using hexane (HEX), dichloromethane (DCM), and methanol (MeOH) solvents to choose the most active one and to identify and isolate a bioactive metabolite. DHS was purified from the HEX extract. The anticonvulsant activity was evaluated with each extract giving an intraperitoneal (i.p.) administration of 100 mg/kg, and 30 min after, seizure behavior was induced by the pentylenetetrazole (PTZ, 85 mg/kg, i.p.) or maximal electroshock stimulation (MES) tests in mice. Molecular docking analysis was performance to reinforce the possible mechanism of action. Our results of seizure behavior and ECoG analysis showed that the HEX extract significantly (p < 0.0001) delayed the onset of seizures and decreased the incidence (p < 0.005) with lower mortality rate (p < 0.05), where DHS was partially responsible for this activity with a possible GABAergic mechanism of action involving the BDZ allosteric site. The MeOH extract also produced a significant effect (p < 0.05) but with less intensity than the HEX extract in the PTZ-induced seizures. A similar anticonvulsant response was observed in the MES test in the presence of DHS (p < 0.005) and the HEX extract (p < 0.05), but not in the case of the MeOH extract. In conclusion, our study provides evidence that non-polar and polar constituents of B. arborea participate in its anticonvulsant property, where DHS might play an important role in the therapy of convulsions mediated by GABAA/BDZ receptor modulation. |
| format | Article |
| id | doaj-art-d9be3ca94d174995adf645980265c9eb |
| institution | DOAJ |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-d9be3ca94d174995adf645980265c9eb2025-08-20T03:11:47ZengElsevierHeliyon2405-84402025-02-01114e4270110.1016/j.heliyon.2025.e42701Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. WatsonJosé David González-Gómez0Samuel Estrada-Soto1Emmanuel Gaona-Tovar2María Eva González-Trujano3Benjamín Villasana-Salazar4Hugo Fernando Narváez-González5David Martínez-Vargas6Laboratorio de Neurofisiología del Control y la Regulación, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz. Calz. México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, Ciudad de México 14370, MexicoFacultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, MexicoFacultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, MexicoLaboratorio de Neurofarmacología de Productos Naturales, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz. México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, Ciudad de México 14370, Mexico; Corresponding author.Laboratorio de Neurofisiología del Control y la Regulación, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz. Calz. México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, Ciudad de México 14370, MexicoHospital de Especialidades Dr. Belisario Domínguez, Av. Tláhuac 4866, San Lorenzo Tezonco, Iztapalapa, Ciudad de México 09930, Mexico; Centro Médico Nacional 20 de Noviembre, Félix Cuevas 540, Col del Valle Sur, Benito Juárez, 03104, Ciudad de México, MexicoLaboratorio de Neurofisiología del Control y la Regulación, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz. Calz. México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, Ciudad de México 14370, Mexico; Corresponding author.Bocconia arborea S. Watson (B. arborea) belongs to the Papaveraceae family and is used in folk medicine because of its depressant effect on the central nervous system. However, its benefits on cerebral paroxysmal activity as an anticonvulsant treatment remain unknown. This study aimed to evaluate the anticonvulsant effect of B. arborea and one of its abundant isolated metabolites dihydrosanguinarine (DHS) by means of experimental models of seizures in mice simultaneously to an electrocorticographic (ECoG) analysis. B. arborea was prepared as extracts with different polarity using hexane (HEX), dichloromethane (DCM), and methanol (MeOH) solvents to choose the most active one and to identify and isolate a bioactive metabolite. DHS was purified from the HEX extract. The anticonvulsant activity was evaluated with each extract giving an intraperitoneal (i.p.) administration of 100 mg/kg, and 30 min after, seizure behavior was induced by the pentylenetetrazole (PTZ, 85 mg/kg, i.p.) or maximal electroshock stimulation (MES) tests in mice. Molecular docking analysis was performance to reinforce the possible mechanism of action. Our results of seizure behavior and ECoG analysis showed that the HEX extract significantly (p < 0.0001) delayed the onset of seizures and decreased the incidence (p < 0.005) with lower mortality rate (p < 0.05), where DHS was partially responsible for this activity with a possible GABAergic mechanism of action involving the BDZ allosteric site. The MeOH extract also produced a significant effect (p < 0.05) but with less intensity than the HEX extract in the PTZ-induced seizures. A similar anticonvulsant response was observed in the MES test in the presence of DHS (p < 0.005) and the HEX extract (p < 0.05), but not in the case of the MeOH extract. In conclusion, our study provides evidence that non-polar and polar constituents of B. arborea participate in its anticonvulsant property, where DHS might play an important role in the therapy of convulsions mediated by GABAA/BDZ receptor modulation.http://www.sciencedirect.com/science/article/pii/S2405844025010825AlkaloidsBocconia arboreaDihydrosanguinarineElectrocorticogramSeizures |
| spellingShingle | José David González-Gómez Samuel Estrada-Soto Emmanuel Gaona-Tovar María Eva González-Trujano Benjamín Villasana-Salazar Hugo Fernando Narváez-González David Martínez-Vargas Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson Heliyon Alkaloids Bocconia arborea Dihydrosanguinarine Electrocorticogram Seizures |
| title | Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson |
| title_full | Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson |
| title_fullStr | Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson |
| title_full_unstemmed | Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson |
| title_short | Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from Bocconia arborea S. Watson |
| title_sort | involvement of gaba bdz receptors in the anticonvulsant effects of dihydrosanguinarine from bocconia arborea s watson |
| topic | Alkaloids Bocconia arborea Dihydrosanguinarine Electrocorticogram Seizures |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025010825 |
| work_keys_str_mv | AT josedavidgonzalezgomez involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT samuelestradasoto involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT emmanuelgaonatovar involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT mariaevagonzaleztrujano involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT benjaminvillasanasalazar involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT hugofernandonarvaezgonzalez involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson AT davidmartinezvargas involvementofgababdzreceptorsintheanticonvulsanteffectsofdihydrosanguinarinefrombocconiaarboreaswatson |