Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis
Background. The rise of highly resistant bacteria creates a persistent urge to develop new antimicrobial agents. This paper investigates the application of the lipopeptide antibiotic daptomycin in infections involving the human bone. Methods. Compressive and tensile strength testing of daptomycin-la...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/578251 |
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| author | Steffen B. Rosslenbroich Michael J. Raschke Carolin Kreis Nancy Tholema-Hans Andreas Uekoetter Rudolf Reichelt Thomas F. Fuchs |
| author_facet | Steffen B. Rosslenbroich Michael J. Raschke Carolin Kreis Nancy Tholema-Hans Andreas Uekoetter Rudolf Reichelt Thomas F. Fuchs |
| author_sort | Steffen B. Rosslenbroich |
| collection | DOAJ |
| description | Background. The rise of highly resistant bacteria creates a persistent urge to develop new antimicrobial agents. This paper investigates the application of the lipopeptide antibiotic daptomycin in infections involving the human bone. Methods. Compressive and tensile strength testing of daptomycin-laden PMMA was performed referring to the ISO 5833. The microstructure of the antibiotic-laden PMMA was evaluated by scanning electron microscopy. Intracellular activity of daptomycin was determined by a human osteoblast infection model. Elution kinetics of the antibiotic-laden bone cement was measured by using a continuous flow chamber setup. Results. There was no significant negative effect of adding 1.225% and 7.5% per weight of daptomycin to the PMMA. There was no significant difference in intracellular activity comparing gentamicin to daptomycin. Elution of daptomycin from PMMA showed within the first-hour initial peak values of 15–20 μg/mL. Conclusion. Daptomycin has a certain degree of activity in the intracellular environment of osteoblasts. Daptomycin admixed to PMMA remains bactericidal and does not significantly impair structural characteristics of the PMMA. The results of this paper suggest that daptomycin might be a potent alternative for treating osteomyelitis and implant-associated infection in trauma and orthopedic surgery caused by multiresistant strains. |
| format | Article |
| id | doaj-art-d9b1a3d30b644c1f80868becefaffe3c |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d9b1a3d30b644c1f80868becefaffe3c2025-08-20T02:21:25ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/578251578251Daptomycin: Local Application in Implant-Associated Infection and Complicated OsteomyelitisSteffen B. Rosslenbroich0Michael J. Raschke1Carolin Kreis2Nancy Tholema-Hans3Andreas Uekoetter4Rudolf Reichelt5Thomas F. Fuchs6Department of Trauma, Hand, and Reconstructive Surgery, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Waldeyerstraße 1, 48149 Muenster, GermanyDepartment of Trauma, Hand, and Reconstructive Surgery, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Waldeyerstraße 1, 48149 Muenster, GermanyDepartment of Trauma, Hand, and Reconstructive Surgery, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Waldeyerstraße 1, 48149 Muenster, GermanyDepartment of Trauma, Hand, and Reconstructive Surgery, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Waldeyerstraße 1, 48149 Muenster, GermanyInstitute of Medical Microbiology, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Domagkstraße 10, 48149 Muenster, GermanyInstitute of Medical Physics and Biophysics, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Robert-Koch-Straße 31, 48149 Muenster, GermanyDepartment of Trauma, Hand, and Reconstructive Surgery, University Hospital Muenster, Westfalian-Wilhelm's-University Muenster, Waldeyerstraße 1, 48149 Muenster, GermanyBackground. The rise of highly resistant bacteria creates a persistent urge to develop new antimicrobial agents. This paper investigates the application of the lipopeptide antibiotic daptomycin in infections involving the human bone. Methods. Compressive and tensile strength testing of daptomycin-laden PMMA was performed referring to the ISO 5833. The microstructure of the antibiotic-laden PMMA was evaluated by scanning electron microscopy. Intracellular activity of daptomycin was determined by a human osteoblast infection model. Elution kinetics of the antibiotic-laden bone cement was measured by using a continuous flow chamber setup. Results. There was no significant negative effect of adding 1.225% and 7.5% per weight of daptomycin to the PMMA. There was no significant difference in intracellular activity comparing gentamicin to daptomycin. Elution of daptomycin from PMMA showed within the first-hour initial peak values of 15–20 μg/mL. Conclusion. Daptomycin has a certain degree of activity in the intracellular environment of osteoblasts. Daptomycin admixed to PMMA remains bactericidal and does not significantly impair structural characteristics of the PMMA. The results of this paper suggest that daptomycin might be a potent alternative for treating osteomyelitis and implant-associated infection in trauma and orthopedic surgery caused by multiresistant strains.http://dx.doi.org/10.1100/2012/578251 |
| spellingShingle | Steffen B. Rosslenbroich Michael J. Raschke Carolin Kreis Nancy Tholema-Hans Andreas Uekoetter Rudolf Reichelt Thomas F. Fuchs Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis The Scientific World Journal |
| title | Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis |
| title_full | Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis |
| title_fullStr | Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis |
| title_full_unstemmed | Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis |
| title_short | Daptomycin: Local Application in Implant-Associated Infection and Complicated Osteomyelitis |
| title_sort | daptomycin local application in implant associated infection and complicated osteomyelitis |
| url | http://dx.doi.org/10.1100/2012/578251 |
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