Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment
Goal. Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine (Vitis spp.) is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in Odesa regio...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine
2025-03-01
|
| Series: | Карантин і захист рослин |
| Subjects: | |
| Online Access: | https://kr.ipp.gov.ua/index.php/journal/article/view/206 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850220367097888768 |
|---|---|
| author | L. Konup M. Riabyi N. Nikolaeva A. Konup V. Chystiakova A. Vuek M. Kyryk V. Vlasov |
| author_facet | L. Konup M. Riabyi N. Nikolaeva A. Konup V. Chystiakova A. Vuek M. Kyryk V. Vlasov |
| author_sort | L. Konup |
| collection | DOAJ |
| description | Goal. Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine (Vitis spp.) is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in Odesa region, to identify the causative agent, to study the possibility of its treatment by the method of water heat treatment and the influence of its different temperature regimes on the physiological parameters of the plant.
Methods. Field — examination of industrial plantations for the presence of symptoms of grapevine short node. Molecular biological method of polymerase chain reaction (PCR) in real time — identification of grapevine fanleaf virus. Research was conducted in accordance with DSTU 8562:2015. The identification of grape short knot virus was carried out in laboratory conditions on the equipment for the PCR laboratory, which undergoes annual calibration.
Results. As a result of the phytosanitary survey of industrial grape plantations in the Odesa region, grape bushes of various varieties with symptoms of short knot were found. With the help of real-time PCR, grape short knot virus was identified. The reaction conditions are selected and recommended. The effect of thermotherapy as a means of improving the health of plants affected by grapevine shortknot virus was studied. The influence of different modes of heat therapy of grape vines and seedlings on the physiological indicators of Vitis vinifera L. material during its preparation for grafting: callus formation, cell germination was studied.
Conclusions. As a result of the conducted phytosanitary survey of vineyards in the Odesa region, grape bushes with symptoms of short knot were found, which amounted to 20%. The PCR method for the identification of grape short knot virus in real time with hybridization-fluorescence detection has been improved. It was established that among all the grape varieties studied, the technical variety Cabernet Sauvignon was the most susceptible to viral infection. It has been proven that with the help of the method of thermotherapy, it is possible to heal grape vines and seedlings that have been affected by the short knot virus of grapes. The optimal mode (50°С with exposure of 30 min.) was selected for water thermotherapy of grape vines, which did not affect their physiological parameters. |
| format | Article |
| id | doaj-art-d9abcdeee0484dc6a6ad36382fcabba5 |
| institution | OA Journals |
| issn | 2312-0614 2786-4979 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine |
| record_format | Article |
| series | Карантин і захист рослин |
| spelling | doaj-art-d9abcdeee0484dc6a6ad36382fcabba52025-08-20T02:07:05ZengInstitute of Plant Protection of the National Academy of Agrarian Sciences of UkraineКарантин і захист рослин2312-06142786-49792025-03-011141910.36495/2312-0614.2025.1.14-19206Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatmentL. Konup0M. Riabyi1N. Nikolaeva2A. Konup3V. Chystiakova4A. Vuek5M. Kyryk6V. Vlasov7National Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineNational Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineNational Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineNational Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineNational Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineNational University of Life and Environmental Sciences of Ukraine, 15, Heroiv Oborony str., 03041, Kyiv, UkraineNational University of Life and Environmental Sciences of Ukraine, 15, Heroiv Oborony str., 03041, Kyiv, UkraineNational Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» of the NAAS, 27, Peremohy str., Tairove settlement, Odesa district, Odesa region, 65496, UkraineGoal. Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine (Vitis spp.) is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in Odesa region, to identify the causative agent, to study the possibility of its treatment by the method of water heat treatment and the influence of its different temperature regimes on the physiological parameters of the plant. Methods. Field — examination of industrial plantations for the presence of symptoms of grapevine short node. Molecular biological method of polymerase chain reaction (PCR) in real time — identification of grapevine fanleaf virus. Research was conducted in accordance with DSTU 8562:2015. The identification of grape short knot virus was carried out in laboratory conditions on the equipment for the PCR laboratory, which undergoes annual calibration. Results. As a result of the phytosanitary survey of industrial grape plantations in the Odesa region, grape bushes of various varieties with symptoms of short knot were found. With the help of real-time PCR, grape short knot virus was identified. The reaction conditions are selected and recommended. The effect of thermotherapy as a means of improving the health of plants affected by grapevine shortknot virus was studied. The influence of different modes of heat therapy of grape vines and seedlings on the physiological indicators of Vitis vinifera L. material during its preparation for grafting: callus formation, cell germination was studied. Conclusions. As a result of the conducted phytosanitary survey of vineyards in the Odesa region, grape bushes with symptoms of short knot were found, which amounted to 20%. The PCR method for the identification of grape short knot virus in real time with hybridization-fluorescence detection has been improved. It was established that among all the grape varieties studied, the technical variety Cabernet Sauvignon was the most susceptible to viral infection. It has been proven that with the help of the method of thermotherapy, it is possible to heal grape vines and seedlings that have been affected by the short knot virus of grapes. The optimal mode (50°С with exposure of 30 min.) was selected for water thermotherapy of grape vines, which did not affect their physiological parameters.https://kr.ipp.gov.ua/index.php/journal/article/view/206phytosanitary inspectionpolymerase chain reactiongrape short knot virusgrapevine fanleaf virusthermotherapy |
| spellingShingle | L. Konup M. Riabyi N. Nikolaeva A. Konup V. Chystiakova A. Vuek M. Kyryk V. Vlasov Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment Карантин і захист рослин phytosanitary inspection polymerase chain reaction grape short knot virus grapevine fanleaf virus thermotherapy |
| title | Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment |
| title_full | Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment |
| title_fullStr | Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment |
| title_full_unstemmed | Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment |
| title_short | Grapevine fanleaf degeneration disease (GFDD) in vineyards in the Odesa region, diagnosis, prospects for its recovery by heat treatment |
| title_sort | grapevine fanleaf degeneration disease gfdd in vineyards in the odesa region diagnosis prospects for its recovery by heat treatment |
| topic | phytosanitary inspection polymerase chain reaction grape short knot virus grapevine fanleaf virus thermotherapy |
| url | https://kr.ipp.gov.ua/index.php/journal/article/view/206 |
| work_keys_str_mv | AT lkonup grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT mriabyi grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT nnikolaeva grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT akonup grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT vchystiakova grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT avuek grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT mkyryk grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment AT vvlasov grapevinefanleafdegenerationdiseasegfddinvineyardsintheodesaregiondiagnosisprospectsforitsrecoverybyheattreatment |