Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes

Anaerobutyricum soehngenii (previously Eubacterium hallii) is a butyrate-producing next-generation beneficial microbe generally recognized as safe. Several short-term intervention trials by A. soehngenii L2–7 have shown improvement of insulin sensitivity in prediabetic subjects and type 2 diabetes p...

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Main Authors: Ilias Attaye, Julia K. Bird, Max Nieuwdorp, Sahin Gül, Jos F. M. L. Seegers, Steven Morrison, Stijn Hofkens, Hilde Herrema, Nam Bui, Marie-Luise Puhlmann, Willem M. de Vos
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Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Gut Microbes
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Online Access:https://www.tandfonline.com/doi/10.1080/19490976.2025.2504115
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author Ilias Attaye
Julia K. Bird
Max Nieuwdorp
Sahin Gül
Jos F. M. L. Seegers
Steven Morrison
Stijn Hofkens
Hilde Herrema
Nam Bui
Marie-Luise Puhlmann
Willem M. de Vos
author_facet Ilias Attaye
Julia K. Bird
Max Nieuwdorp
Sahin Gül
Jos F. M. L. Seegers
Steven Morrison
Stijn Hofkens
Hilde Herrema
Nam Bui
Marie-Luise Puhlmann
Willem M. de Vos
author_sort Ilias Attaye
collection DOAJ
description Anaerobutyricum soehngenii (previously Eubacterium hallii) is a butyrate-producing next-generation beneficial microbe generally recognized as safe. Several short-term intervention trials by A. soehngenii L2–7 have shown improvement of insulin sensitivity in prediabetic subjects and type 2 diabetes patients. To determine the long-term cardiometabolic benefits and safety, we performed a 3-month double-blind, randomized placebo-controlled intervention in 98 prediabetic insulin-resistant adults in Europe and U.S. with daily administration of encapsulated cells of A. soehngenii CH-106, a tetracycline-sensitive isogenic derivative of strain L2–7. Compared to placebo, A. soehngenii-treated subjects showed significantly reduced glycemic variability (1% reduction in the coefficient of variation; p = 0.01) and improved glycemic control (6% reduction in the overall net glycemic action-1; p < 0.05), including reduced serum glycated hemoglobin (HbA1c) levels when including the 4-week washout period (1 mmol/mol reduction; p < 0.05). Moreover, diastolic blood pressure was significantly reduced in all A. soehngenii-treated subjects (3 mm Hg; p < 0.05). The study product was well-tolerated and had no effect on the global intestinal microbiota composition, including alpha and beta-diversity, besides an increased abundance of A. soehngenii in the treatment group, indicative of compliance. The U.S. participants, compared to those in Europe, responded best, notably in the oral glucose tolerance tests (15% improvement in the area-under-the curve of plasma glucose levels; p = 0.039) or coefficient of variation (reduction of 3.1%; p < 0.05). This potentially relates to a more severe prediabetic state in U.S. subjects, associated with significantly reduced (1.5–3.5-fold) relative abundance of Bifidobacterium, Coprococcus, Ruminococcus spp. and two-fold increased relative abundance of Lachnoclostridium spp. In conclusion, daily oral supplementation with A. soehngenii was safe and improved various markers of glycemic control, reduced HbA1c levels and diastolic blood pressure, indicating a novel microbiome-based approach to improve cardio-metabolic health in adults at risk for developing type 2 diabetes.Clinical trial reg. no. NCT04529473, clinicaltrials.govSocial media summary 120 characters: Anaerobutyricum soehngenii supplementation improves #cardio-metabolic health in subjects at risk for type 2 #diabetes
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spelling doaj-art-d9aa7cd443d54750927a42eda8c26a3c2025-08-20T02:31:27ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2504115Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetesIlias Attaye0Julia K. Bird1Max Nieuwdorp2Sahin Gül3Jos F. M. L. Seegers4Steven Morrison5Stijn Hofkens6Hilde Herrema7Nam Bui8Marie-Luise Puhlmann9Willem M. de Vos10Department of Internal and Vascular Medicine, Amsterdam University Medical Centers, The NetherlandsBird Scientific Writing, Wassenaar, The NetherlandsDepartment of Internal and Vascular Medicine, Amsterdam University Medical Centers, The NetherlandsCaelus Health, Zegveld, The NetherlandsCaelus Health, Zegveld, The NetherlandsP2F Ventures, Alton, UKCaelus Health, Zegveld, The NetherlandsDepartment of Internal and Vascular Medicine, Amsterdam University Medical Centers, The NetherlandsDepartment of Internal and Vascular Medicine, Amsterdam University Medical Centers, The NetherlandsLaboratory of Microbiology, Wageningen University, Wageningen, The NetherlandsDepartment of Internal and Vascular Medicine, Amsterdam University Medical Centers, The NetherlandsAnaerobutyricum soehngenii (previously Eubacterium hallii) is a butyrate-producing next-generation beneficial microbe generally recognized as safe. Several short-term intervention trials by A. soehngenii L2–7 have shown improvement of insulin sensitivity in prediabetic subjects and type 2 diabetes patients. To determine the long-term cardiometabolic benefits and safety, we performed a 3-month double-blind, randomized placebo-controlled intervention in 98 prediabetic insulin-resistant adults in Europe and U.S. with daily administration of encapsulated cells of A. soehngenii CH-106, a tetracycline-sensitive isogenic derivative of strain L2–7. Compared to placebo, A. soehngenii-treated subjects showed significantly reduced glycemic variability (1% reduction in the coefficient of variation; p = 0.01) and improved glycemic control (6% reduction in the overall net glycemic action-1; p < 0.05), including reduced serum glycated hemoglobin (HbA1c) levels when including the 4-week washout period (1 mmol/mol reduction; p < 0.05). Moreover, diastolic blood pressure was significantly reduced in all A. soehngenii-treated subjects (3 mm Hg; p < 0.05). The study product was well-tolerated and had no effect on the global intestinal microbiota composition, including alpha and beta-diversity, besides an increased abundance of A. soehngenii in the treatment group, indicative of compliance. The U.S. participants, compared to those in Europe, responded best, notably in the oral glucose tolerance tests (15% improvement in the area-under-the curve of plasma glucose levels; p = 0.039) or coefficient of variation (reduction of 3.1%; p < 0.05). This potentially relates to a more severe prediabetic state in U.S. subjects, associated with significantly reduced (1.5–3.5-fold) relative abundance of Bifidobacterium, Coprococcus, Ruminococcus spp. and two-fold increased relative abundance of Lachnoclostridium spp. In conclusion, daily oral supplementation with A. soehngenii was safe and improved various markers of glycemic control, reduced HbA1c levels and diastolic blood pressure, indicating a novel microbiome-based approach to improve cardio-metabolic health in adults at risk for developing type 2 diabetes.Clinical trial reg. no. NCT04529473, clinicaltrials.govSocial media summary 120 characters: Anaerobutyricum soehngenii supplementation improves #cardio-metabolic health in subjects at risk for type 2 #diabeteshttps://www.tandfonline.com/doi/10.1080/19490976.2025.2504115Anaerobutyricumnext-gen beneficial microbesbutyrate-producing bacteriaprediabetestype 2 diabetescontinuous glucose measurement
spellingShingle Ilias Attaye
Julia K. Bird
Max Nieuwdorp
Sahin Gül
Jos F. M. L. Seegers
Steven Morrison
Stijn Hofkens
Hilde Herrema
Nam Bui
Marie-Luise Puhlmann
Willem M. de Vos
Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
Gut Microbes
Anaerobutyricum
next-gen beneficial microbes
butyrate-producing bacteria
prediabetes
type 2 diabetes
continuous glucose measurement
title Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
title_full Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
title_fullStr Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
title_full_unstemmed Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
title_short Anaerobutyricum soehngenii improves glycemic control and other markers of cardio-metabolic health in adults at risk of type 2 diabetes
title_sort anaerobutyricum soehngenii improves glycemic control and other markers of cardio metabolic health in adults at risk of type 2 diabetes
topic Anaerobutyricum
next-gen beneficial microbes
butyrate-producing bacteria
prediabetes
type 2 diabetes
continuous glucose measurement
url https://www.tandfonline.com/doi/10.1080/19490976.2025.2504115
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