Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing
We are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesized from poly(3,4-ethylenedioxythiophene) (PEDOT)....
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MDPI AG
2025-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/6/384 |
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| author | Christopher Animashaun Abdellatif Ait Lahcen Gymama Slaughter |
| author_facet | Christopher Animashaun Abdellatif Ait Lahcen Gymama Slaughter |
| author_sort | Christopher Animashaun |
| collection | DOAJ |
| description | We are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesized from poly(3,4-ethylenedioxythiophene) (PEDOT). The LIG electrode offers a highly porous, conductive scaffold, while electrodeposited AuNPs enhance catalytic activity and signal amplification. The PEDOT-based MIP layer, electropolymerized via cyclic voltammetry, imparts molecular specificity by creating lactate-specific binding sites. Cyclic voltammetry confirmed successful molecular imprinting and enhanced interfacial electron transfer. The resulting LIG/AuNPs/MIP biosensor demonstrated a wide linear detection range from 0.1 µM to 2500 µM, with a sensitivity of 22.42 µA/log(µM) and a low limit of detection (0.035 µM). The sensor showed excellent selectivity against common electroactive interferents such as glucose and uric acid, long-term stability, and accurate recovery in artificial saliva (>95.7%), indicating strong potential for practical application. This enzyme-free platform offers a robust and scalable strategy for continuous lactate monitoring, particularly suited for wearable devices in sports performance monitoring and critical care diagnostics. |
| format | Article |
| id | doaj-art-d993cb3db28c48d78b49dc03e2f428fd |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-d993cb3db28c48d78b49dc03e2f428fd2025-08-20T03:26:21ZengMDPI AGBiosensors2079-63742025-06-0115638410.3390/bios15060384Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate SensingChristopher Animashaun0Abdellatif Ait Lahcen1Gymama Slaughter2Center for Bioelectronics, Old Dominion University, Norfolk, VA 23508, USACenter for Bioelectronics, Old Dominion University, Norfolk, VA 23508, USACenter for Bioelectronics, Old Dominion University, Norfolk, VA 23508, USAWe are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesized from poly(3,4-ethylenedioxythiophene) (PEDOT). The LIG electrode offers a highly porous, conductive scaffold, while electrodeposited AuNPs enhance catalytic activity and signal amplification. The PEDOT-based MIP layer, electropolymerized via cyclic voltammetry, imparts molecular specificity by creating lactate-specific binding sites. Cyclic voltammetry confirmed successful molecular imprinting and enhanced interfacial electron transfer. The resulting LIG/AuNPs/MIP biosensor demonstrated a wide linear detection range from 0.1 µM to 2500 µM, with a sensitivity of 22.42 µA/log(µM) and a low limit of detection (0.035 µM). The sensor showed excellent selectivity against common electroactive interferents such as glucose and uric acid, long-term stability, and accurate recovery in artificial saliva (>95.7%), indicating strong potential for practical application. This enzyme-free platform offers a robust and scalable strategy for continuous lactate monitoring, particularly suited for wearable devices in sports performance monitoring and critical care diagnostics.https://www.mdpi.com/2079-6374/15/6/384lactate biosensorlaser-induced graphenegold nanoparticlesmolecularly imprinted polymerPEDOT |
| spellingShingle | Christopher Animashaun Abdellatif Ait Lahcen Gymama Slaughter Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing Biosensors lactate biosensor laser-induced graphene gold nanoparticles molecularly imprinted polymer PEDOT |
| title | Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing |
| title_full | Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing |
| title_fullStr | Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing |
| title_full_unstemmed | Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing |
| title_short | Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode for Non-Enzymatic Lactate Sensing |
| title_sort | gold nanoparticle enhanced molecularly imprinted polymer electrode for non enzymatic lactate sensing |
| topic | lactate biosensor laser-induced graphene gold nanoparticles molecularly imprinted polymer PEDOT |
| url | https://www.mdpi.com/2079-6374/15/6/384 |
| work_keys_str_mv | AT christopheranimashaun goldnanoparticleenhancedmolecularlyimprintedpolymerelectrodefornonenzymaticlactatesensing AT abdellatifaitlahcen goldnanoparticleenhancedmolecularlyimprintedpolymerelectrodefornonenzymaticlactatesensing AT gymamaslaughter goldnanoparticleenhancedmolecularlyimprintedpolymerelectrodefornonenzymaticlactatesensing |